ASAP3 (NM_001143778) Human Tagged ORF Clone

CAT#: RG226781

ASAP3 (GFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ASAP3
• Synonyms: ACAP4; CENTB6; DDEFL1; UPLC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226781 representing NM_001143778
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAGCAGTTCAGCGTCGCCGAGTTCCTGGCCGTCACCGCGGAGGACCTCAGCTCCCCGGCTGGGG
  CCGCCGCCTTCGCCGCCAAGATGCCCCGGTACCGAGGGGCGGCGCTGGCGCGGGAGGAGATCTTGGAAGG
  AGACCAAGCCATCCTGCAGAGAATAAAGAAGGCTGTGCGGGCAATCCATAGCTCCGGCCTTGGCCATGTG
  GAGAATGAAGAGCAGTACCGAGAGGCCGTGGAATCCTTAGGCAACAGCCACCTGTCCCAGAACAGCCATG
  AGCTGTCCACAGGCTTCCTAAACTTGGCCGTGTTCACCCGCGAGGTTGCTGCGCTCTTCAAGAACCTGAT
  TCAGAACTTGAACAACATTGTCTCTTTCCCCCTGGACAGTCTGATGAAGGGGCAGCTGAGGGACGGTCGA
  CAGGCTTCTCTTTCCTTGGGGTCTAGGGCCAAGCTGGAGAAGGAGCGCGATCGGGCCAGGGTGACAGGAG
  GGATCCCTGGGGAGGTGGCCCAGGACATGCAGAGAGAGCGGCGCATCTTCCAGCTGCACATGTGTGAGTA
  TCTGCTCAAAGCCGGGGAGAGCCAGATGAAGCAAGGTCCTGACTTCCTTCAGAGCCTCATCAAGTTCTTC
  CACGCCCAGCACAACTTTTTCCAAGATGGCTGGAAGGCTGCCCAGAGCCTGTTCCCCTTCATCGAGAAGC
  TGGCGGCCTCAGTACATGCACTCCATCAGGCCCAGGAGGACGAGCTACAGAAGCTGACCCAGCTCCGGGA
  CTCCCTCCGAGGGACACTGCAGCTTGAGAGCAGAGAGGAACACCTGAGCCGGAAGAACTCAGGATGTGGC
  TATAGCATCCACCAGCACCAAGGCAACAAGCAGTTTGGGACGGAGAAAGTGGGCTTTCTATACAAGAAAA
  GTGACGGAATTCGAAGAGTCTGGCAGAAAAGGAAGTGTGGAGTCAAGTATGGCTGCCTGACCATCTCACA
  CAGCACGATAAACCGGCCCCCGGTGAAGCTGACCCTGCTGACGTGCCAAGTGAGGCCAAACCCTGAGGAG
  AAAAAGTGCTTCGACCTGGTGACCCACAACCGGACGTACCACTTTCAGGCAGAGGACGAGCACGAGTGTG
  AGGCGTGGGTGTCAGTGTTGCAGAACAGCAAGGACGAAGCCCTGAGCAGCGCCTTCCTCGGGGAGCCCAG
  CGCTGGCCCGGGGTCCTGGGGGTCCGCCGGCCATGATGGGGAGCCGCACGACCTCACAAAGCTGCTCATC
  GCGGAGGTGAAGAGCAGGCCTGGGAATAGCCAGTGCTGCGACTGCGGGGCTGCAGACCCCACGTGGCTCA
  GCACCAACCTGGGCGTGCTCACCTGCATCCAGTGCTCGGGCGTCCACCGCGAACTGGGCGTGCGCTTTTC
  GCGCATGCAGTCACTCACCTTGGACCTGCTGGGCCCCTCCGAGTTGTTGCTGGCCTTGAACATGGGAAAC
  ACGAGCTTCAATGAGGTCATGGAGGCCCAGCTACCCTCACACGGCGGCCCTAAACCCTCAGCTGAGAGTG
  ACATGGGCACCCGCAGGGACTACATTATGGCCAAGTATGTGGAGCATAGGTTTGCACGCCGGTGCACACC
  TGAGCCTCAGCGACTCTGGACAGCCATTTGCAACAGGGACCTCCTGTCGGTACTGGAGGCCTTTGCCAAT
  GGGCAGGACTTTGGACAGCCGCTGCCAGGGCCTGATGCACAGGCACCTGAAGAACTCGTCTTGCATTTGG
  CTGTCAAAGTCGCCAACCAGGCTTCCCTGCCTCTGGTGGATTTCATCATCCAGAACGGTGGTCACCTGGA
  TGCCAAGGCTGCTGACGGGAACACGGCTCTGCACTACGCAGCACTCTACAACCAGCCCGACTGCCTCAAG
  CTGCTGCTGAAGGGGAGAGCTTTGGTTGGCACAGTAAATGAAGCAGGCGAGACAGCTCTGGACATAGCCA
  GGAAGAAGCACCACAAGGAGTGTGAGGAGCTGCTGGAGCAGGCCCAGGCGGGGACCTTTGCCTTCCCTCT
  ACATGTGGACTACTCCTGGGTAATTTCCACAGAGCCTGGCTCTGACAGTGAGGAGGATGAGGAAGAGAAG
  CGCTGCTTGCTGAAGCTCCCGGCCCAGGCTCACTGGGCCAGTGGGAGGCTGGACATCAGCAACAAGACCT
  ATGAGACTGTCGCCAGCCTGGGAGCAGCCACCCCTCAGGGCGAGAGTGAGGACTGTCCCCCGCCCTTGCC
  AGTCAAAAACTCTTCTCGGACTTTGGTCCAAGGGTGTGCAAGACATGCCAGTGGAGATCGTTCTGAAGTC
  TCCAGCCTGAGTTCAGAGGCCCCTGAGACCCCTGAGAGCCTGGGCAGTCCAGCCTCCTCCTCCAGTCTGA
  TGAGCCCCTTGGAACCTGGGGATCCCAGCCAAGCCCCACCCAACTCTGAAGAGGGCCTCCGAGAGCCCCC
  AGGCACCTCCAGACCCAGCCTGACATCCGGGACCACCCCTTCGGAGATGTACCTCCCCGTCAGATTCAGC
  TCCGAGAGCACTCGCTCCTATCGGCGGGGGGCGCGGAGCCCTGAAGATGGTCCCTCAGCCAGGCAGCCTC
  TGCCCAGAAGGAACGTGCCGGTTGGCATCACTGAAGGAGATGGCTCAAGGACTGGGAGTCTCCCAGCAAG
  TTCTGTGCAACTTTTGCAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226781 representing NM_001143778
  Red=Cloning site Green=Tags(s)
  
  MPEQFSVAEFLAVTAEDLSSPAGAAAFAAKMPRYRGAALAREEILEGDQAILQRIKKAVRAIHSSGLGHV
  ENEEQYREAVESLGNSHLSQNSHELSTGFLNLAVFTREVAALFKNLIQNLNNIVSFPLDSLMKGQLRDGR
  QASLSLGSRAKLEKERDRARVTGGIPGEVAQDMQRERRIFQLHMCEYLLKAGESQMKQGPDFLQSLIKFF
  HAQHNFFQDGWKAAQSLFPFIEKLAASVHALHQAQEDELQKLTQLRDSLRGTLQLESREEHLSRKNSGCG
  YSIHQHQGNKQFGTEKVGFLYKKSDGIRRVWQKRKCGVKYGCLTISHSTINRPPVKLTLLTCQVRPNPEE
  KKCFDLVTHNRTYHFQAEDEHECEAWVSVLQNSKDEALSSAFLGEPSAGPGSWGSAGHDGEPHDLTKLLI
  AEVKSRPGNSQCCDCGAADPTWLSTNLGVLTCIQCSGVHRELGVRFSRMQSLTLDLLGPSELLLALNMGN
  TSFNEVMEAQLPSHGGPKPSAESDMGTRRDYIMAKYVEHRFARRCTPEPQRLWTAICNRDLLSVLEAFAN
  GQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLK
  LLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEK
  RCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEV
  SSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPSEMYLPVRFS
  SESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143778
• ORF Size: 2682 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143778.1, NP_001137250.1
• RefSeq Size: 4172
• RefSeq ORF: 2685
• Locus ID: 55616
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]