PCDH15 (NM_001142769) Human Tagged ORF Clone

CAT#: RG226910

PCDH15 (GFP-tagged) - Human protocadherin-related 15 (PCDH15), transcript variant I

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PCDH15
• Synonyms: CDHR15; DFNB23; USH1F
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226910 representing NM_001142769
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCGACAGTTTTATCTCTGGACATGTTTAGCTTCAGGGATCATCCTGGGCTCTCTCTTTGAAATCT
  GCTTGGGCCAGTATGATGATGACTGGCAATATGAGGATTGCAAACTAGCTAGGGGAGGACCACCAGCTAC
  CATAGTTGCTATTGATGAAGAAAGTCGGAATGGTACAATTCTGGTGGACAACATGCTGATCAAAGGGACT
  GCTGGAGGACCAGACCCCACCATAGAACTTTCTTTAAAGGATAATGTGGATTACTGGGTGTTGATGGATC
  CTGTTAAGCAAATGCTTTTCCTGAACAGCACCGGAAGAGTTCTGGATAGAGATCCACCGATGAACATACA
  CTCCATTGTGGTGCAGGTCCAGTGCATCAACAAAAAAGTGGGCACTATTATCTACCATGAAGTGCGAATA
  GTGGTGAGAGACAGGAATGACAACTCACCCACTTTCAAGCATGAAAGCTACTATGCCACAGTGAATGAGC
  TCACTCCAGTTGGTACCACAATATTCACAGGATTTTCAGGAGACAATGGAGCTACAGATATAGATGATGG
  ACCAAATGGACAGATAGAGTATGTTATTCAGTATAATCCAGATGATCCGACATCCAATGACACCTTTGAA
  ATTCCCCTAATGTTGACTGGAAATATAGTGTTAAGGAAGAGGCTCAACTATGAAGATAAGACTCGCTACT
  TTGTCATAATCCAAGCTAATGACCGTGCCCAAAATCTGAATGAGAGGCGAACCACCACCACCACTCTCAC
  AGTGGATGTTCTGGATGGAGATGACTTGGGTCCAATGTTTCTTCCTTGTGTCCTTGTGCCAAACACTCGT
  GATTGCCGTCCACTCACTTATCAAGCTGCCATACCTGAGTTGAGAACTCCGGAAGAACTGAACCCCATTA
  TTGTTACGCCACCAATCCAAGCCATTGATCAGGACCGGAATATTCAACCGCCATCAGATAGGCCAGGAAT
  CCTCTATTCCATCCTTGTTGGGACTCCTGAGGATTACCCACGATTTTTCCATATGCATCCTAGGACAGCA
  GAACTTAGTCTCCTGGAGCCAGTAAACAGAGACTTTCACCAGAAATTTGATTTGGTTATTAAGGCTGAAC
  AAGACAATGGTCATCCTCTTCCTGCCTTTGCCGGTCTACACATTGAAATACTGGATGAAAACAATCAAAG
  TCCATATTTTACAATGCCCAGTTATCAAGGCTATATCCTGGAATCTGCCCCAGTGGGAGCAACCATTTCG
  GACAGTCTCAATTTGACTTCACCTTTAAGAATAGTAGCTCTGGACAAGGACATAGAAGATGTTCCACCCA
  GTGGAGTTCCTACAAAAGACCCAGAGCTTCACCTTTTTCTGAATGACTACACCTCAGTCTTCACCGTCAC
  ACAGACTGGTATTACTCGCTACCTCACCTTACTTCAACCAGTGGACAGGGAAGAACAGCAAACTTACACC
  TTTTCGATAACAGCATTTGATGGTGTACAAGAAAGTGAGCCAGTCATCGTCAATATTCAAGTGATGGATG
  CAAATGATAACACGCCAACCTTCCCTGAAATATCCTATGATGTGTATGTTTATACAGACATGAGACCTGG
  GGACAGTGTCATACAGCTCACTGCAGTCGACGCAGACGAAGGGTCAAATGGGGAGATCACATATGAAATC
  CTTGTTGGGGCTCAGGGAGACTTCATCATCAATAAAACAACAGGGCTTATCACCATCGCTCCAGGGGTGG
  AAATGATAGTCGGGCGGACTTACGCACTCACGGTCCAAGCAGCGGATAATGCTCCTCCTGCAGAGCGAAG
  GAACTCCATCTGCACTGTGTATATTGAAGTGCTTCCACCAAATAATCAAAGCCCTCCTCGCTTCCCACAG
  CTGATGTATAGCCTTGAAATTAGTGAAGCCATGAGGGTTGGTGCTGTTTTATTAAATCTACAGGCAACTG
  ATCGAGAGGGAGACTCAATAACATATGCCATTGAGAATGGAGATCCTCAGAGAGTTTTTAATCTTTCAGA
  AACCACGGGGATTCTAACCTTAGGGAAAGCACTGGACAGGGAAAGCACTGATCGCTACATTCTGATCATC
  ACAGCTTCAGATGGCAGGCCAGATGGGACCTCAACTGCCACAGTAAACATAGTGGTGACAGATGTCAATG
  ACAATGCTCCAGTGTTTGATCCTTATCTGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTT
  TGTGGGTCAAGTAAAAGCAACAGACCCTGATGCTGGAATAAATGGTCAAGTGCACTACAGTTTGGGTAAC
  TTTAATAATCTTTTTCGTATCACATCCAATGGGAGCATTTACACAGCAGTGAAGCTTAACAGAGAAGTCA
  GGGACTACTATGAACTTGTTGTTGTGGCAACAGATGGAGCAGTACACCCTCGTCATTCAACTCTAACCTT
  GGCCATCAAGGTTTTGGACATTGATGATAACAGTCCTGTGTTCACCAATTCAACATACACTGTCCTTGTT
  GAAGAGAATTTGCCAGCTGGGACTACCATCCTTCAAATAGAGGCCAAAGATGTCGACCTTGGAGCAAATG
  TGTCTTACCGGATAAGAAGCCCAGAAGTGAAGCACTTTTTTGCACTACATCCATTTACAGGAGAACTATC
  GCTTTTAAGGAGTTTAGATTATGAGGCATTTCCAGACCAAGAAGCAAGTATCACTTTTCTGGTAGAGGCC
  TTTGATATTTATGGAACAATGCCACCTGGTATTGCTACTGTCACAGTGATTGTAAAGGATATGAATGATT
  ATCCTCCTGTCTTTAGTAAACGAATATACAAAGGGATGGTGGCTCCGGATGCAGTCAAGGGTACACCTAT
  CACAACAGTTTATGCTGAAGATGCAGACCCTCCTGGATTACCTGCAAGTCGTGTGAGGTATAGAGTAGAT
  GATGTACAGTTTCCTTACCCTGCCAGTATTTTTGAAGTGGAAGAAGATTCTGGAAGAGTAATAACACGAG
  TCAATCTTAATGAAGAACCTACAACAATTTTTAAGTTGGTGGTGGTTGCTTTTGATGATGGGGAGCCTGT
  GATGTCCAGCAGTGCCACAGTGAAGATTCTTGTCTTACATCCTGGTGAGATCCCACGCTTCACACAGGAG
  GAATATAGACCTCCTCCAGTAAGTGAACTTGCCACCAAAGGGACCATGGTTGGTGTAATTTCTGCTGCTG
  CCATTAATCAAAGTATTGTGTACTCCATTGTTTCAGGAAATGAAGAAGATACATTTGGAATTAATAACAT
  CACAGGTGTTATCTATGTGAATGGACCTCTGGATTATGAGACCAGGACAAGCTATGTACTTCGAGTCCAA
  GCTGATTCCCTGGAAGTGGTCCTTGCCAATCTCCGAGTTCCTTCAAAAAGCAATACAGCTAAAGTATACA
  TTGAGATTCAGGATGAAAATAATCATCCCCCAGTGTTTCAGAAAAAATTCTACATCGGAGGTGTATCTGA
  AGATGCAAGAATGTTTACTTCTGTACTCAGAGTGAAGGCTACTGATAAAGATACTGGCAATTATAGTGTC
  ATGGCCTACAGACTCATAATACCACCAATTAAAGAGGGAAAAGAAGGATTTGTAGTGGAAACATATACAG
  GGCTTATCAAAACTGCTATGCTCTTCCATAATATGAGGAGATCCTACTTCAAGTTTCAAGTTATTGCAAC
  TGACGACTATGGGAAGGGACTGAGCGGCAAAGCCGATGTACTCGTCTCCGTGGTCAATCAGCTGGATATG
  CAAGTCATTGTTTCCAATGTGCCTCCTACTCTAGTGGAAAAAAAGATAGAAGATCTTACAGAGATCTTGG
  ATCGCTATGTTCAGGAACAAATTCCTGGTGCCAAGGTCGTAGTGGAGTCCATTGGAGCTCGCCGGCATGG
  AGATGCCTTTTCCCTAGAAGATTACACCAAATGTGACTTGACTGTCTATGCAATTGACCCCCAAACCAAC
  AGAGCCATCGATAGAAATGAGCTTTTTAAATTTTTGGATGGCAAACTACTTGATATCAATAAAGACTTTC
  AGCCGTATTATGGGGAAGGAGGACGCATTCTGGAGATCCGGACTCCAGAGGCAGTGACCAGCATTAAAAA
  GAGAGGAGAAAGTCTAGGATACACAGAAGGGGCCTTGTTGGCTCTGGCCTTCATCATCATCCTCTGCTGC
  ATTCCTGCCATCTTGGTGGTTTTGGTCAGCTACAGACAGTTTAAAGTACGTCAAGCTGAGTGTACAAAGA
  CTGCACGAATTCAGGCCGCATTACCCGCGGCTAAACCAGCAGTGCCGGCTCCTGCACCAGTGGCAGCGCC
  CCCGCCGCCGCCGCCGCCTCCGCCAGGTGCGCATCTCTATGAAGAACTTGGAGACAGCTCAATGCATAAG
  TATGAAATGCCTCAATATGGGAGTCGCCGTCGATTGTTACCACCAGCTGGACAGGAGGAATATGGTGAGG
  TGGTTGGTGAAGCTGAGGAAGAATATGAGGAGGAAGAGGAAGAGCCAAAGAAAATTAAAAAACCAAAGGT
  TGAAATTAGAGAGCCTAGTGAGGAGGAAGAAGTAGTTGTAACTATCGAAAAACCACCAGCAGCTGAGCCT
  ACATACACAACATGGAAGAGAGCCAGAATATTCCCCATGATTTTTAAGAAAGTTAGAGGATTAGCTGATA
  AAAGAGGAATCGTTGACCTTGAGGGTGAAGAGTGGCAGAGACGCCTTGAGGAAGAAGATAAAGATTATTT
  GAAACTCACTCTGGACCAAGAGGAAGCAACAGAAAGCACTGTAGAATCAGAGGAGGAATCCTCCAGCGAC
  TATACTGAATACAGTGAAGAAGAGTCTGAGTTCAGTGAGTCTGAGACTACAGAAGAGGAATCTGAGTCAG
  AGACACCCTCTGAGGAGGAGGAGAGTTCCACCCCTGAATCAGAAGAATCGGAATCCACAGAGTCAGAAGG
  AGAAAAAGCAAGGAAAAACATTGTGCTTGCAAGAAGAAGGCCCATGGTTGAGGAGGTCAAGGAAGTCAAG
  GGTAGGAAAGAGGAGCCACAAGAAGAACAAAAAGAACCTAAGATGGAAGAAGAAGAACACTCAGAAGAAG
  AAGAAAGTGGACCAGCCCCTGTGGAAGAAAGTACAGACCCTGAAGCTCAAGATATCCCTGAAGAGGGCAG
  TGCAGAATCAGCTTCGGTGGAAGGAGGTGTGGAAAGTGAGGAGGAATCAGAATCAGGTAGTAGTAGCAGT
  AGTAGCGAAAGTCAGTCTGGAGGTCCATGGGGCTATCAGGTACCAGCGTATGACAGAAGCAAGAATGCAA
  ACCAAAAGAAGTCGCCAGGAGCAAACTCTGAAGGTTACAACACAGCACTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226910 representing NM_001142769
  Red=Cloning site Green=Tags(s)
  
  MFRQFYLWTCLASGIILGSLFEICLGQYDDDWQYEDCKLARGGPPATIVAIDEESRNGTILVDNMLIKGT
  AGGPDPTIELSLKDNVDYWVLMDPVKQMLFLNSTGRVLDRDPPMNIHSIVVQVQCINKKVGTIIYHEVRI
  VVRDRNDNSPTFKHESYYATVNELTPVGTTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPDDPTSNDTFE
  IPLMLTGNIVLRKRLNYEDKTRYFVIIQANDRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTR
  DCRPLTYQAAIPELRTPEELNPIIVTPPIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFFHMHPRTA
  ELSLLEPVNRDFHQKFDLVIKAEQDNGHPLPAFAGLHIEILDENNQSPYFTMPSYQGYILESAPVGATIS
  DSLNLTSPLRIVALDKDIEDVPPSGVPTKDPELHLFLNDYTSVFTVTQTGITRYLTLLQPVDREEQQTYT
  FSITAFDGVQESEPVIVNIQVMDANDNTPTFPEISYDVYVYTDMRPGDSVIQLTAVDADEGSNGEITYEI
  LVGAQGDFIINKTTGLITIAPGVEMIVGRTYALTVQAADNAPPAERRNSICTVYIEVLPPNNQSPPRFPQ
  LMYSLEISEAMRVGAVLLNLQATDREGDSITYAIENGDPQRVFNLSETTGILTLGKALDRESTDRYILII
  TASDGRPDGTSTATVNIVVTDVNDNAPVFDPYLPRNLSVVEEEANAFVGQVKATDPDAGINGQVHYSLGN
  FNNLFRITSNGSIYTAVKLNREVRDYYELVVVATDGAVHPRHSTLTLAIKVLDIDDNSPVFTNSTYTVLV
  EENLPAGTTILQIEAKDVDLGANVSYRIRSPEVKHFFALHPFTGELSLLRSLDYEAFPDQEASITFLVEA
  FDIYGTMPPGIATVTVIVKDMNDYPPVFSKRIYKGMVAPDAVKGTPITTVYAEDADPPGLPASRVRYRVD
  DVQFPYPASIFEVEEDSGRVITRVNLNEEPTTIFKLVVVAFDDGEPVMSSSATVKILVLHPGEIPRFTQE
  EYRPPPVSELATKGTMVGVISAAAINQSIVYSIVSGNEEDTFGINNITGVIYVNGPLDYETRTSYVLRVQ
  ADSLEVVLANLRVPSKSNTAKVYIEIQDENNHPPVFQKKFYIGGVSEDARMFTSVLRVKATDKDTGNYSV
  MAYRLIIPPIKEGKEGFVVETYTGLIKTAMLFHNMRRSYFKFQVIATDDYGKGLSGKADVLVSVVNQLDM
  QVIVSNVPPTLVEKKIEDLTEILDRYVQEQIPGAKVVVESIGARRHGDAFSLEDYTKCDLTVYAIDPQTN
  RAIDRNELFKFLDGKLLDINKDFQPYYGEGGRILEIRTPEAVTSIKKRGESLGYTEGALLALAFIIILCC
  IPAILVVLVSYRQFKVRQAECTKTARIQAALPAAKPAVPAPAPVAAPPPPPPPPPGAHLYEELGDSSMHK
  YEMPQYGSRRRLLPPAGQEEYGEVVGEAEEEYEEEEEEPKKIKKPKVEIREPSEEEEVVVTIEKPPAAEP
  TYTTWKRARIFPMIFKKVRGLADKRGIVDLEGEEWQRRLEEEDKDYLKLTLDQEEATESTVESEEESSSD
  YTEYSEEESEFSESETTEEESESETPSEEEESSTPESEESESTESEGEKARKNIVLARRRPMVEEVKEVK
  GRKEEPQEEQKEPKMEEEEHSEEEESGPAPVEESTDPEAQDIPEEGSAESASVEGGVESEEESESGSSSS
  SSESQSGGPWGYQVPAYDRSKNANQKKSPGANSEGYNTAL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142769
• ORF Size: 5370 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142769.1, NP_001136241.1
• RefSeq Size: 5768
• RefSeq ORF: 5373
• Locus ID: 65217
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008]