APLP2 (NM_001142278) Human Tagged ORF Clone

CAT#: RG227009

APLP2 (GFP-tagged) - Human amyloid beta (A4) precursor-like protein 2 (APLP2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: APLP2
• Synonyms: APLP-2; APPH; APPL2; CDEBP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227009 representing NM_001142278
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCTGGTGGGGCTCA
  CGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCTGT
  TGCTGAGCCTCAAATCGCAATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGAAATGG
  GAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAGTTCTTCAGTACTGTCAGGAGA
  TGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGGTGCCG
  GAGGGACAAAAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTTCCTCCAACTCCTCTG
  CCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGCATGCTCGCTTCCAGAAGG
  CTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAGGCAGA
  GCTTCAAGCTAAGAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATGGTTAAA
  GCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAGACCCACCTGGCCCGAGTGGAAGCTA
  TGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCGCCACGGCC
  TCATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACATACCATCCGT
  CATTACCAGCATGTGTTGGCTGTTGACCCAGAAAAGGCGGCCCAGATGAAATCCCAGGTGATGACACATC
  TCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTATGTAGCCCAAGA
  AATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTCACTGCCTCAATC
  TCAGAGACCCCTGTGGACGTCCGGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCGTTCCACCCCTTCC
  ACCCCTTCCCAGCCCTACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGATGGGGGACTGATCGGTGC
  CGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCATTGACGAGACTCTGGATGTT
  AAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAATCCGTGGGCCCACTGC
  GGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCAGTGGCCATTGCCACGGT
  CATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAGTATGGCACCATCAGCCACGGGATCGTGGAGGTT
  GATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCATGGCTATGAGAACCCCACCT
  ACAAATACCTGGAGCAGATGCAGATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227009 representing NM_001142278
  Red=Cloning site Green=Tags(s)
  
  MAATGTAAAAATGRLLLLLLVGLTAPALALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKW
  EPDPTGTKSCFETKEEVLQYCQEMYPELQITNVMEANQRVSIDNWCRRDKKQCKSRFVTPFKCLVPPTPL
  PTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAKNLPKAERQTLIQHFQAMVK
  ALEKEAASEKQQLVETHLARVEAMLNDRRRMALENYLAALQSDPPRPHRILQALRRYVRAENKDRLHTIR
  HYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTASI
  SETPVDVRVSSEESEEIPPFHPFHPFPALPENEGSGVGEQDGGLIGAEEKVINSKNKVDENMVIDETLDV
  KEMIFNAERVGGLEEERESVGPLREDFSLSSSALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGIVEV
  DPMLTPEERHLNKMQNHGYENPTYKYLEQMQI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142278
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142278.1, NP_001135750.1
• RefSeq Size: 3104 bp
• RefSeq ORF: 1569 bp
• Locus ID: 334
• Cytogenetics: 11q24.3
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]'