IMPDH1 (NM_001142574) Human Tagged ORF Clone
CAT#: RG227194
- TrueORF®
IMPDH1 (GFP-tagged) - Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 6
"NM_001142574" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | IMPDH1 |
Synonyms | IMPD; IMPD1; IMPDH-I; LCA11; RP10; sWSS2608 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227194 representing NM_001142574
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACTACCTGATCAGCGGCGGCACCGGCTACGTGCCCGAGGATGGGCTCACCGCGCAGCAGCTCT TCGCCAGCGCCGACGGCCTCACCTACAACGACTTCCTGATTCTCCCAGGATTCATAGACTTCATAGCTGA TGAGGTGGACCTGACCTCAGCCCTGACCCGGAAGATCACGCTGAAGACGCCACTGATCTCCTCCCCCATG GACACTGTGACAGAGGCTGACATGGCCATTGCCATGGCTCTGATGGGAGGTATTGGTTTCATTCACCACA ACTGCACCCCAGAGTTCCAGGCCAACGAGAAGTTTGAACAGGGCTTCATCACGGACCCTGTGGTGCTGAG CCCCTCGCACACTGTGGGCGATGTGCTGGAGGCCAAGATGCGGCATGGCTTCTCTGGCATCCCCATCACT GAGACGGGCACCATGGGCAGCAAGCTGGTGGGCATCGTCACCTCCCGAGACATCGACTTTCTTGCTGAGA AGGACCACACCACCCTCCTCAGTGAGGTGATGACGCCAAGGATTGAACTGGTGGTGGCTCCAGCAGGTGT GACGTTGAAAGAGGCAAATGAGATCCTGCAGCGTAGCAAGAAAGGGAAGCTGCCTATCGTCAATGATTGC GATGAGCTGGTGGCCATCATCGCCCGCACCGACCTGAAGAAGAACCGAGACTACCCTCTGGCCTCCAAGG ATTCCCAGAAGCAGCTGCTCTGTGGGGCAGCTGTGGGCACCCGTGAGGATGACAAATACCGTCTGGACCT GCTCACCCAGGCGGGCGTCGACGTCATAGTCTTGGACTCGTCCCAAGGGAATTCGGTGTATCAGATCGCC ATGGTGCATTACATCAAACAGAAGTACCCCCACCTCCAGGTGATTGGGGGGAACGTGGTGACAGCAGCCC AGGCCAAGAACCTGATTGATGCTGGTGTGGACGGGCTGCGCGTGGGCATGGGCTGCGGCTCCATCTGCAT CACCCAGGAAGTGATGGCCTGTGGTCGGCCCCAGGGCACTGCTGTGTACAAGGTGGCTGAGTATGCCCGG CGCTTTGGTGTGCCCATCATAGCCGATGGCGGCATCCAGACCGTGGGACACGTGGTCAAGGCCCTGGCCC TTGGAGCCTCCACAGTGATGATGGGCTCCCTGCTGGCCGCCACTACGGAGGCCCCTGGCGAGTACTTCTT CTCAGACGGGGTGCGGCTCAAGAAGTACCGGGGCATGGGCTCACTGGATGCCATGGAGAAGAGCAGCAGC AGCCAGAAACGATACTTCAGCGAGGGGGATAAAGTGAAGATCGCGCAGGGTGTCTCGGGCTCCATCCAGG ACAAAGGATCCATTCAGAAGTTCGTGCCCTACCTCATAGCAGGCATCCAACACGGCTGCCAGGATATCGG GGCCCGCAGCCTGTCTGTCCTTCGGTCCATGATGTACTCAGGAGAGCTCAAGTTTGAGAAGCGGACCATG TCGGCCCAGATTGAGGGTGGTGTCCATGGCCTGCACTCTTACGAAAAGCGGCTGTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227194 representing NM_001142574
Red=Cloning site Green=Tags(s) MADYLISGGTGYVPEDGLTAQQLFASADGLTYNDFLILPGFIDFIADEVDLTSALTRKITLKTPLISSPM DTVTEADMAIAMALMGGIGFIHHNCTPEFQANEKFEQGFITDPVVLSPSHTVGDVLEAKMRHGFSGIPIT ETGTMGSKLVGIVTSRDIDFLAEKDHTTLLSEVMTPRIELVVAPAGVTLKEANEILQRSKKGKLPIVNDC DELVAIIARTDLKKNRDYPLASKDSQKQLLCGAAVGTREDDKYRLDLLTQAGVDVIVLDSSQGNSVYQIA MVHYIKQKYPHLQVIGGNVVTAAQAKNLIDAGVDGLRVGMGCGSICITQEVMACGRPQGTAVYKVAEYAR RFGVPIIADGGIQTVGHVVKALALGASTVMMGSLLAATTEAPGEYFFSDGVRLKKYRGMGSLDAMEKSSS SQKRYFSEGDKVKIAQGVSGSIQDKGSIQKFVPYLIAGIQHGCQDIGARSLSVLRSMMYSGELKFEKRTM SAQIEGGVHGLHSYEKRLY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001142574 |
ORF Size | 1527 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142574.1, NP_001136046.1 |
RefSeq Size | 2380 bp |
RefSeq ORF | 1530 bp |
Locus ID | 3614 |
Cytogenetics | 7q32.1 |
Protein Families | Druggable Genome |
Protein Pathways | Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism |
Gene Summary | 'The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC326005 | IMPDH1 (untagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 6 |
USD 870.00 |
|
RC227194 | IMPDH1 (Myc-DDK-tagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 6 |
USD 480.00 |
|
RC227194L3 | Lenti-ORF clone of IMPDH1 (Myc-DDK-tagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 6 |
USD 680.00 |
|
RC227194L4 | Lenti-ORF clone of IMPDH1 (mGFP-tagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 6 |
USD 680.00 |
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