ULK2 (NM_001142610) Human Tagged ORF Clone

CAT#: RG227297

ULK2 (GFP-tagged) - Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ULK2
• Synonyms: ATG1B; Unc51.2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227297 representing NM_001142610
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGTGGGTGACTTCGAGTACAGCAAGAGGGATCTCGTGGGACACGGGGCCTTCGCCGTGGTCT
  TCCGGGGGCGGCACCGCCAGAAAACTGATTGGGAGGTAGCTATTAAAAGTATTAATAAAAAGAACTTGTC
  AAAATCACAAATACTGCTTGGAAAGGAAATTAAAATCTTAAAGGAACTTCAGCATGAAAATATTGTAGCA
  CTCTATGATGTTCAGGAATTACCCAACTCTGTCTTTTTGGTGATGGAGTATTGCAATGGTGGAGACCTCG
  CAGATTATTTGCAAGCGAAAGGGACTCTCAGTGAAGACACGATCAGAGTGTTTCTGCATCAGATTGCTGC
  TGCCATGCGAATCCTGCACAGCAAAGGAATCATCCACAGAGATCTCAAACCACAGAACATCTTGCTGTCC
  TATGCCAATCGCAGAAAATCAAGTGTCAGTGGTATTCGCATCAAAATAGCGGATTTTGGTTTTGCTCGTT
  ACCTACATAGTAACATGATGGCTGCAACACTGTGTGGATCCCCGATGTACATGGCTCCTGAGGTTATTAT
  GTCTCAACATTATGATGCTAAGGCTGACTTGTGGAGCATAGGAACAGTGATATACCAATGCCTAGTTGGA
  AAACCACCTTTTCAGGCCAATAGTCCTCAAGACTTAAGGATGTTTTATGAAAAAAACAGGAGCTTAATGC
  CTAGTATTCCCAGAGAAACATCACCTTATTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAACCAAAA
  AGATAGAATGGACTTTGAAGCGTTTTTTAGCCATCCTTTTCTTGAGCAAGGTCCAGTAAAAAAATCTTGC
  CCAGTTCCAGTGCCCATGTATTCTGGTTCTGTCTCTGGAAGCTCCTGTGGCAGCTCTCCATCTTGTCGTT
  TTGCTTCTCCACCATCCCTTCCAGATATGCAGCATATTCAGGAAGAAAACTTATCTTCCCCACCATTGGG
  TCCTCCCAACTATCTACAAGTTTCCAAAGATTCTGCCAGTACTAGTAGCAAGAACTCTTCTTGTGACACG
  GATGACTTTGTTTTGGTGCCACACAACATCTCGTCAGACCACTCATGTGATATGCCAATGGGGACTGCTG
  GCAGACGTGCTTCAAATGAATTCTTGGTGTGTGGAGGGCAGTGTCAGCCTACTGTGTCACCTCACAGCGA
  AACAGCACCAATTCCAGTTCCTACTCAAATAAGGAATTATCAGCGCATAGAGCAGAATCTTACATCTACT
  GCCAGCTCAGGCACAAATGTACATGGTTCTCCAAGATCTGCAGTGGTACGAAGGTCCAACACCAGCCCCA
  TGGGCTTCCTCCGGCCGGGATCATGCTCCCCAGTACCAGCAGACACAGCACAGACAGTTGGACGAAGGCT
  CTCCACTGGGTCTTCTAGGCCTTACTCACCTTCCCCTTTGGTTGGTACCATTCCTGAGCAATTCAGTCAG
  TGCTGCTGTGGGCATCCTCAGGGCCATGACTCCAGGAGTAGAAACTCCTCAGGTTCTCCAGTGCCACAAG
  CTCAGTCCCCACAGTCTCTCTTATCGGGTGCTAGACTGCAGAGCGCCCCCACCCTCACTGACATCTATCA
  GAACAAGCAGAAGCTCAGAAAACAGCACTCTGACCCCGTGTGCCCATCCCATACTGGGGCTGGGTACAGC
  TACTCGCCTCAGCCCAGTCGGCCTGGCAGCCTTGGAACTTCTCCCACCAAGCACTTGGGGTCCTCTCCAC
  GGAGTTCTGACTGGTTCTTTAAAACTCCTTTGCCAACAATCATTGGCTCTCCTACTAAGACCACAGCTCC
  TTTCAAAATCCCTAAAACTCAAGCATCTTCCAACCTGTTAGCCTTGGTTACTCGTCATGGGCCTGCTGAA
  GAACAGTCGAAAGATGGGAATGAGCCACGGGAATGTGCCCATTGCCTCTTAGTGCAAGGAAGTGAGAGGC
  AGCGGGCCGAGCAGCAGAGCAAGGCAGTGTTTGGCAGATCTGTCAGTACCGGGAAGTTATCAGATCAACA
  AGGAAAGACTCCTATATGTCGACATCAGGGCAGCACAGACAGTTTAAATACAGAACGACCAATGGATATA
  GCTCCGGCAGGAGCCTGTGGTGGTGTTCTGGCACCTCCTGCAGGTACAGCAGCAAGTTCCAAGGCTGTCC
  TCTTCACTGTAGGGTCTCCTCCACACAGTGCGGCAGCCCCCACTTGTACCCACATGTTCCTTCGAACAAG
  AACAACCTCAGTGGGGCCCAGCAACTCCGGGGGCTCTCTTTGTGCCATGAGTGGCCGCGTGTGCGTGGGG
  TCCCCGCCTGGCCCAGGCTTCGGCTCTTCCCCTCCAGGAGCAGAGGCAGCTCCCAGCCTGAGATACGTGC
  CTTACGGTGCTTCACCCCCCAGCCTAGAGGGGCTCATCACCTTTGAAGCCCCTGAACTGCCGGAGGAGAC
  GCTGATGGAGCGGGAACACACAGACACCTTACGCCATCTGAATGTGATGCTGATGTTCACTGAGTGTGTG
  CTGGACCTGACAGCCATGAGGGGAGGAAACCCTGAGCTGTGCACATCTGCTGTGTCCTTGTACCAGATCC
  AGGAGAGTGTGGTGGTGGACCAGATCAGTCAGCTGAGCAAAGACTGGGGGCGGGTGGAGCAGCTGGTGTT
  GTACATGAAAGCAGCACAGCTGCTTGCGGCTTCTCTGCATCTTGCCAAAGCCCAGATCAAGTCCGGGAAA
  CTGAGCCCATCCACAGCTGTGAAACAAGTTGTCAAGAATCTGAACGAACGATATAAATTCTGCATCACCA
  TGTGCAAGAAACTTACAGAAAAGCTGAATCGATTCTTCTCTGACAAACAGAGGTTTATTGATGAAATCAA
  CAGTGTGACTGCAGAGAAACTCATCTATAATTGTGCTGTAGAAATGGTTCAGTCTGCAGCCCTGGATGAG
  ATGTTTCAGCAGACCGAAGATATTGTTTATCGCTATCATAAGGCAGCCCTTCTTTTGGAAGGCCTAAGTA
  GGATTCTACAGGACCCTGCAGATATTGAAAATGTGCATAAATATAAATGTAGTATTGAGAGAAGACTGTC
  GGCGCTCTGCCATAGCACCGCAACCGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227297 representing NM_001142610
  Red=Cloning site Green=Tags(s)
  
  MEVVGDFEYSKRDLVGHGAFAVVFRGRHRQKTDWEVAIKSINKKNLSKSQILLGKEIKILKELQHENIVA
  LYDVQELPNSVFLVMEYCNGGDLADYLQAKGTLSEDTIRVFLHQIAAAMRILHSKGIIHRDLKPQNILLS
  YANRRKSSVSGIRIKIADFGFARYLHSNMMAATLCGSPMYMAPEVIMSQHYDAKADLWSIGTVIYQCLVG
  KPPFQANSPQDLRMFYEKNRSLMPSIPRETSPYLANLLLGLLQRNQKDRMDFEAFFSHPFLEQGPVKKSC
  PVPVPMYSGSVSGSSCGSSPSCRFASPPSLPDMQHIQEENLSSPPLGPPNYLQVSKDSASTSSKNSSCDT
  DDFVLVPHNISSDHSCDMPMGTAGRRASNEFLVCGGQCQPTVSPHSETAPIPVPTQIRNYQRIEQNLTST
  ASSGTNVHGSPRSAVVRRSNTSPMGFLRPGSCSPVPADTAQTVGRRLSTGSSRPYSPSPLVGTIPEQFSQ
  CCCGHPQGHDSRSRNSSGSPVPQAQSPQSLLSGARLQSAPTLTDIYQNKQKLRKQHSDPVCPSHTGAGYS
  YSPQPSRPGSLGTSPTKHLGSSPRSSDWFFKTPLPTIIGSPTKTTAPFKIPKTQASSNLLALVTRHGPAE
  EQSKDGNEPRECAHCLLVQGSERQRAEQQSKAVFGRSVSTGKLSDQQGKTPICRHQGSTDSLNTERPMDI
  APAGACGGVLAPPAGTAASSKAVLFTVGSPPHSAAAPTCTHMFLRTRTTSVGPSNSGGSLCAMSGRVCVG
  SPPGPGFGSSPPGAEAAPSLRYVPYGASPPSLEGLITFEAPELPEETLMEREHTDTLRHLNVMLMFTECV
  LDLTAMRGGNPELCTSAVSLYQIQESVVVDQISQLSKDWGRVEQLVLYMKAAQLLAASLHLAKAQIKSGK
  LSPSTAVKQVVKNLNERYKFCITMCKKLTEKLNRFFSDKQRFIDEINSVTAEKLIYNCAVEMVQSAALDE
  MFQQTEDIVYRYHKAALLLEGLSRILQDPADIENVHKYKCSIERRLSALCHSTATV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142610
• ORF Size: 3108 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142610.1, NP_001136082.1
• RefSeq Size: 3924
• RefSeq ORF: 3111
• Locus ID: 9706
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway, Regulation of autophagy
• Gene Summary: This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008]