SGT1 (ECD) (NM_001135753) Human Tagged ORF Clone

CAT#: RG227341

ECD (GFP-tagged) - Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ECD
• Synonyms: GCR2; HSGT1; SGT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227341 representing NM_001135753
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTGATACCAGATG
  AGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAGAGAATAATCACTCGGTTTGC
  ACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTCAATCTTAAATATAAACCTGGGAAAGGAGGT
  GTTCCTGCTCATATGTTTGGCGTGACAAAGTTTGGGGATAACATTGAGGATGAATGGTTTATTGTTTATG
  TAATAAAGCAGATCACAAAGGAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAATTCTT
  GTTAATAGAAGCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTATTTTTC
  TGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTACCCACCACAC
  CCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAAATACTTGCTTCAGAATCTAT
  ACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAAAAAATTCAGGCCTCACTTCATCGAGCACAC
  TGCTTCCTTCCAGCTGGCATTGTGGCAGTGCTAAAGCAGCGCCCCAGATTGGTGGCTGCAGCAGTCCAGG
  CATTTTACCTACGAGACCCTATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTGCCTGAAACACG
  AATAATGACATCGGCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTTTCTGACTGCAAG
  AAATCCCTTGTGACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTGAAAAAGAATGATTACTTTA
  AGGGACTGATAGAAGGTTCTGCTCAGTACCGGGAAAGGCTAGAAATGGCAGAGAATTACTTCCAGCTCTC
  AGTAGACTGGCCAGAAAGTTCTCTTGCTATGAGCCCTGGTGAAGAAATCTTAACCTTATTACAGACAATA
  CCATTTGATATAGAAGACCTTAAGAAAGAAGCAGCTAATCTTCCCCCAGAGGATGATGACCAGTGGTTAG
  ATCTCTCACCAGATCAGCTGGACCAGCTGCTGCAGGAAGCTGTTGGCAAAAAAGAATCCGAGTCTGTTTC
  CAAGGAGGAGAAGGAGCAGAACTATGACTTAACTGAAGTCTCAGAGAGCATGAAAGCTTTCATATCCAAA
  GTCTCAACCCACAAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCAATCACTTTTGATGCAGATT
  CTTTTCTTAATTATTTTGATAAGATTTTAGGGCCAAGGCCTAATGAGTCAGATTCTGATGATCTGGATGA
  TGAAGACTTTGAATGTTTAGATAGTGATGATGACTTGGACTTTGAAACACACGAACCTGGCGAAGAGGCT
  TCCCTGAAAGGAACACTTGATAATCTCAAGTCATACATGGCCCAGATGGACCAGGAACTAGCACACACCT
  GCATCAGCAAAAGTTTCACCACTAGGAACCAAGTGGAACCTGTATCCCAGACTACCGATAACAATTCAGA
  TGAGGAAGATTCTGGTACGGGAGAATCTGTTATGGCACCAGTAGATGTAGACCTGAACCTGGTTTCAAAT
  ATATTGGAATCCTATAGCTCCCAAGCTGGACTGGCAGGACCTGCTTCCAATCTTTTACAAAGCATGGGAG
  TGCAGCTGCCTGACAACACCGATCACAGACCAACAAGTAAGCCAACAAAAAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227341 representing NM_001135753
  Red=Cloning site Green=Tags(s)
  
  MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGG
  VPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFF
  CHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAH
  CFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSAHGFEILCSKCSPHFSDCK
  KSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLSVDWPESSLAMSPGEEILTLLQTI
  PFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISK
  VSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEA
  SLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSN
  ILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135753
• ORF Size: 1803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001135753.1, NP_001129225.1
• RefSeq Size: 2151
• RefSeq ORF: 1806
• Locus ID: 11319
• Protein Families: Druggable Genome, Transcription Factors