DENN (MADD) (NM_001135944) Human Tagged ORF Clone

CAT#: RG227386

MADD (GFP-tagged) - Human MAP-kinase activating death domain (MADD), transcript variant 10

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MADD
• Synonyms: DENN; IG20; RAB3GEP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227386 representing NM_001135944
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAAAAGAAGAAGTTCTGTCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCACCCGA
  GCAGTGATAGCGTGGCCCAGACTCCTGAATTGCTACGGCGATACCCCTTGGAGGATCACACTGAGTTTCC
  CCTGCCCCCAGATGTAGTGTTCTTCTGCCAGCCCGAGGGCTGCCTGAGCGTGCGGCAGCGGCGCATGAGC
  CTTCGGGATGATACCTCTTTTGTCTTCACCCTCACTGACAAGGACACTGGAGTCACGCGATATGGCATCT
  GTGTTAACTTCTACCGCTCCTTCCAAAAGCGAATCTCTAAGGAGAAGGGGGAAGGTGGGGCAGGGTCCCG
  TGGGAAGGAAGGAACCCATGCCACCTGTGCCTCAGAAGAGGGTGGCACTGAGAGCTCAGAGAGTGGCTCA
  TCCCTGCAGCCTCTCAGTGCTGACTCTACCCCTGATGTGAACCAGTCTCCTCGGGGCAAACGCCGGGCCA
  AGGCGGGGAGCCGCTCCCGCAACAGTACTCTCACGTCCCTGTGCGTGCTCAGCCACTACCCTTTCTTCTC
  CACCTTCCGAGAGTGTTTGTATACTCTCAAGCGCCTGGTGGACTGCTGTAGTGAGCGCCTTCTGGGCAAG
  AAACTGGGCATCCCTCGAGGCGTACAAAGGGACACCATGTGGCGGATCTTTACTGGATCGCTGCTGGTAG
  AGGAGAAGTCAAGTGCCCTTCTGCATGACCTTCGAGAGATTGAGGCCTGGATCTATCGATTGCTGCGCTC
  CCCAGTACCCGTCTCTGGGCAGAAGCGAGTAGACATCGAGGTCCTACCCCAAGAGCTCCAGCCAGCTCTG
  ACCTTTGCTCTTCCAGACCCATCTCGATTCACCCTAGTGGATTTCCCACTGCACCTTCCCTTGGAACTTC
  TAGGTGTGGACGCCTGTCTCCAGGTGCTAACCTGCATTCTGTTAGAGCACAAGGTGGTGCTACAGTCCCG
  AGACTACAATGCACTCTCCATGTCTGTGATGGCATTCGTGGCAATGATCTACCCACTGGAGTATATGTTT
  CCTGTCATCCCGCTGCTACCCACCTGCATGGCATCAGCAGAGCAGCTGCTGTTGGCTCCAACCCCGTACA
  TCATTGGGGTTCCTGCCAGCTTCTTCCTCTACAAACTGGACTTCAAAATGCCTGATGATGTATGGCTAGT
  GGATCTGGACAGCAATAGGGTGATTGCCCCCACCAATGCAGAAGTGCTGCCTATCCTGCCAGAACCAGAA
  TCACTAGAGCTGAAAAAGCATTTAAAGCAGGCCTTGGCCAGCATGAGTCTCAACACCCAGCCCATCCTCA
  ATCTGGAGAAATTTCATGAGGGCCAGGAGATCCCCCTTCTCTTGGGAAGGCCTTCTAATGACCTGCAGTC
  CACACCGTCCACTGAATTCAACCCACTCATCTATGGCAATGATGTGGATTCTGTGGATGTTGCAACCAGG
  GTTGCCATGGTACGGTTCTTCAATTCCGCCAACGTGCTGCAGGGATTTCAGATGCACACGCGTACCCTGC
  GCCTCTTTCCTCGGCCTGTGGTAGCTTTTCAAGCTGGCTCCTTTCTAGCCTCACGTCCCCGGCAGACTCC
  TTTTGCCGAGAAATTGGCCAGGACTCAGGCTGTGGAGTACTTTGGGGAATGGATCCTTAACCCCACCAAC
  TATGCCTTTCAGCGAATTCACAACAATATGTTTGATCCAGCCCTGATTGGTGACAAGCCAAAGTGGTATG
  CTCATCAGCTGCAGCCTATCCACTATCGCGTCTATGACAGCAATTCCCAGCTGGCTGAGGCCCTGAGTGT
  ACCACCAGAGCGGGACTCTGACTCCGAACCTACTGATGATAGTGGCAGTGATAGTATGGATTATGACGAT
  TCAAGCTCTTCTTACTCCTCCCTTGGTGACTTTGTCAGTGAAATGATGAAATGTGACATTAATGGTGATA
  CTCCCAATGTGGACCCTCTGACACATGCAGCACTGGGGGATGCCAGCGAGGTGGAGATTGACGAGCTGCA
  GAATCAGAAGGAAGCAGAAGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAAAACCCCCCACTGCGCTCC
  AGCTCTAGCACCACAGCCAGCAGCAGCCCCAGCACTGTCATCCACGGAGCCAACTCTGAACCTGCTGACT
  CTACGGAGATGGATGATAAGGCAGCAGTAGGCGTCTCCAAGCCCCTCCCTTCCGTGCCTCCCAGCATTGG
  CAAATCGAACGTGGACAGACGTCAGGCAGAAATTGGAGAGGGGGCTCAAAAGCTGCTGCGGCCCAACAGC
  TTGAGACTGGCAAGTGACTCAGATGCAGAGTCAGACTCTCGGGCAAGCTCTCCCAACTCCACCGTCTCCA
  ACACCAGCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTATCGGAACCACAGTACCAG
  CTTCAGTCTTTCAAACCTCACACTGCCCACCAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTG
  AAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAAAGAG
  AACCTCCATCACCCCAGGGTCGATCCAGCAATTCTAGTGAGAACCAGCAGTTCCTGAAGGAGGTGGTGCA
  CAGCGTGCTGGACGGCCAGGGAGTTGGCTGGCTCAACATGAAAAAGGTGCGCCGGCTGCTGGAGAGCGAG
  CAGCTGCGAGTCTTTGTCCTGAGCAAGCTGAACCGCATGGTGCAGTCAGAGGACGATGCCCGGCAGGACA
  TCATCCCGGATGTGGAGATCAGTCGGAAGGTGTACAAGGGAATGTTAGACCTCCTCAAGTGTACAGTCCT
  CAGCTTGGAGCAGTCCTATGCCCACGCGGGTCTGGGTGGCATGGCCAGCATCTTTGGGCTTTTGGAGATT
  GCCCAGACCCACTACTATAGTAAAGAACCAGACAAGCGGAAGAGAAGTCCAACAGAAAGTGTAAATACCC
  CAGTTGGCAAGGATCCTGGCCTAGCTGGGCGGGGGGACCCAAAGGCTATGGCACAACTGAGAGTTCCACA
  ACTGGGACCTCGGGCACCAAGTGCCACAGGAAAGGGTCCTAAGGAACTGGACACCAGAAGTTTAAAGGAA
  GAAAATTTTATAGCATCTATTGGGCCTGAAGTAATCAAACCTGTCTTTGACCTTGGTGAGACAGAGGAGA
  AAAAGTCCCAGATCAGCGCAGACAGTGGTGTGAGCCTGACGTCTAGTTCCCAGAGGACTGATCAAGACTC
  TGTCATCGGCGTGAGTCCAGCTGTTATGATCCGCAGCTCAAGTCAGGATTCTGAAGTGAGTAATAGCTCT
  GGAGAGACCCTTGGAGCTGACAGTGACTTGAGCAGCAATGCAGGTGATGGACCAGGTGGCGAGGGCAGTG
  TTCACCTGGCAAGCTCTCGGGGCACTTTGTCTGATAGTGAAATTGAGACCAACTCTGCCACAAGCACCAT
  CTTTGGTAAAGCCCACAGCTTGAAGCCAAGCATAAAGGAGAAGCTGGCAGGCAGCCCCATTCGTACTTCT
  GAAGATGTGAGCCAGCGAGTCTATCTCTATGAGGGACTCCTAGGCAAAGAGCGTTCTACTTTATGGGACC
  AAATGCAATTCTGGGAAGATGCCTTCTTAGATGCTGTGATGTTGGAGAGAGAAGGGATGGGTATGGACCA
  GGGTCCCCAGGAAATGATCGACAGGTACCTGTCCCTTGGAGAACATGACCGGAAGCGCCTGGAAGATGAT
  GAAGATCGCTTGCTGGCCACACTTCTGCACAACCTCATCTCCTACATGCTGCTGATGAAGGTAAATAAGA
  ATGACATCCGCAAGAAGGTGAGGCGCCTAATGGGAAAGTCGCACATTGGGCTTGTGTACAGCCAGCAAAT
  CAATGAGGTGCTTGATCAGCTGGCGAACCTGAATGGACGCGATCTCTCTATCTGGTCCAGTGGCAGCCGG
  CACATGAAGAAGCAGACATTTGTGGTACATGCAGGGACAGATACAAACGGAGATATCTTTTTCATGGAGG
  TGTGCGATGACTGTGTGGTGTTGCGTAGTAACATCGGAACAGTGTATGAGCGCTGGTGGTACGAGAAGCT
  CATCAACATGACCTACTGTCCCAAGACGAAGGTGTTGTGCTTGTGGCGTAGAAATGGCTCTGAGACCCAG
  CTCAACAAGTTCTATACTAAAAAGTGTCGGGAGCTGTACTACTGTGTGAAGGACAGCATGGAGCGCGCTG
  CCGCCCGACAGCAAAGCATCAAACCCGGACCTGAATTGGGTGGCGAGTTCCCTGTGCAGGACCTGAAGAC
  TGGTGAGGGTGGCCTGCTGCAGGTGACCCTGGAAGGGATCAACCTCAAATTCATGCACAATCAGGTTTTC
  ATAGAGCTGAATCACATTAAAAAGTGCAATACAGTTCGAGGCGTCTTTGTCCTGGAGGAATTTGTTCCTG
  AAATTAAAGAAGTGGTGAGCCACAAGTACAAGACACCAATGGCCCACGAAATCTGCTACTCCGTATTATG
  TCTCTTCTCGTACGTGGCTGCAGTTCATAGCAGTGAGGAAGATCTCAGAACCCCGCCCCGGCCTGTCTCT
  AGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227386 representing NM_001135944
  Red=Cloning site Green=Tags(s)
  
  MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMS
  LRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGEGGAGSRGKEGTHATCASEEGGTESSESGS
  SLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGK
  KLGIPRGVQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPAL
  TFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMF
  PVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPE
  SLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATR
  VAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTN
  YAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDD
  SSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKEAEEPGPDSENSQENPPLRS
  SSSTTASSSPSTVIHGANSEPADSTEMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNS
  LRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKATPFPSL
  KGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESE
  QLRVFVLSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEI
  AQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKE
  ENFIASIGPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVSNSS
  GETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSPIRTS
  EDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDD
  EDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSR
  HMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQ
  LNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQVF
  IELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVS
  S
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135944
• ORF Size: 4623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001135944.1, NP_001129416.1
• RefSeq Size: 5713
• RefSeq ORF: 4626
• Locus ID: 8567
• Protein Families: Druggable Genome
• Gene Summary: Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]