NELL2 (NM_001145107) Human Tagged ORF Clone

CAT#: RG227443

NELL2 (GFP-tagged) - Human NEL-like 2 (chicken) (NELL2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NELL2
• Synonyms: NRP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227443 representing NM_001145107
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTCCCTCCTTTGCTTAAAGGGCAGGCGAGCGCTACTCGCTCCAGCCTTGCCTCCTGCAGCTGGG
  TGGTCTTTTTTCTCTCCTGTCTTTCAAGACACGCGCCCGAAATCGAGGGAGGGAGACGATGGACTGAGCT
  GATCCGCACCATGGAGTCTCGGGTCTTACTGAGAACATTCTGTTTGATCTTCGGTCTCGGAGCAGTTTGG
  GGGCTTGGTGTGGACCCTTCCCTACAGATTGACGTCTTAACAGAGTTAGAACTTGGGGAGTCCACGACCG
  GAGTGCGTCAGGTCCCGGGGCTGCATAATGGGACGAAAGCCTTTCTCTTTCAAGATACTCCCAGAAGCAT
  AAAAGCATCCACTGCTACAGCTGAACAGTTTTTTCAGAAGCTGAGAAATAAACATGAATTTACTATTTTG
  GTGACCCTAAAACAGACCCACTTAAATTCAGGAGTTATTCTCTCAATTCACCACTTGGATCACAGGTACC
  TGGAACTGGAAAGTAGTGGCCATCGGAATGAAGTCAGACTGCATTACCGCTCAGGCAGTCACCGCCCTCA
  CACAGAAGTGTTTCCTTACATTTTGGCTGATGACAAGTGGCACAAGCTCTCCTTAGCCATCAGTGCTTCC
  CATTTGATTTTACACATTGACTGCAATAAAATTTATGAAAGGGTAGTAGAAAAGCCCTCCACAGACTTGC
  CTCTAGGCACAACATTTTGGCTAGGACAGAGAAATAATGCGCATGGATATTTTAAGGGTATAATGCAAGA
  TGTCCAATTACTTGTCATGCCCCAGGGATTTATTGCTCAGTGCCCAGATCTTAATCGCACCTGTCCAACT
  TGCAATGACTTCCATGGACTTGTGCAGAAAATCATGGAGCTACAGGATATTTTAGCCAAAACATCAGCCA
  AGCTGTCTCGAGCTGAACAGCGAATGAATAGATTGGATCAGTGCTATTGTGAAAGGACTTGCACCATGAA
  GGGAACCACCTACCGAGAATTTGAGTCCTGGATAGACGGCTGTAAGAACTGCACATGCCTGAATGGAACC
  ATCCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGGCTCTTGCGTATGTGGATG
  GCAAATGCTGTAAGGAATGCAAATCGATATGCCAATTTCAAGGACGAACCTACTTTGAAGGAGAAAGAAA
  TACAGTCTATTCCTCTTCTGGAGTATGTGTTCTCTATGAGTGCAAGGACCAGACCATGAAACTTGTTGAG
  AGTTCAGGCTGTCCAGCTTTGGATTGTCCAGAGTCTCATCAGATAACCTTGTCTCACAGCTGTTGCAAAG
  TTTGTAAAGGTTATGACTTTTGTTCTGAAAGGCATAACTGCATGGAGAATTCCATCTGCAGAAATCTGAA
  TGACAGGGCTGTTTGTAGCTGTCGAGATGGTTTTAGGGCTCTTCGAGAGGATAATGCCTACTGTGAAGAC
  ATCGATGAGTGTGCTGAAGGGCGCCATTACTGTCGTGAAAATACAATGTGTGTCAACACCCCGGGTTCTT
  TTATGTGCATCTGCAAAACTGGATACATCAGAATTGATGATTATTCATGTACAGAACATGATGAGTGTAT
  CACAAATCAGCACAACTGTGATGAAAATGCTTTATGCTTCAACACTGTTGGAGGACACAACTGTGTTTGC
  AAGCCGGGCTATACAGGGAATGGAACGACATGCAAAGCATTTTGCAAAGATGGCTGTAGGAATGGAGGAG
  CCTGTATTGCCGCTAATGTGTGTGCCTGCCCACAAGGCTTCACTGGACCCAGCTGTGAAACGGACATTGA
  TGAATGCTCTGATGGTTTTGTTCAATGTGACAGTCGTGCTAATTGCATTAACCTGCCTGGATGGTACCAC
  TGTGAGTGCAGAGATGGCTACCATGACAATGGGATGTTTTCACCAAGTGGAGAATCGTGTGAAGATATTG
  ATGAGTGTGGGACCGGGAGGCACAGCTGTGCCAATGATACCATTTGCTTCAATTTGGATGGCGGATATGA
  TTGTCGATGTCCTCATGGAAAGAATTGCACAGGGGACTGCATCCATGATGGAAAAGTTAAGCACAATGGT
  CAGATTTGGGTGTTGGAAAATGACAGGTGCTCTGTGTGCTCATGTCAGAATGGATTCGTTATGTGTCGAC
  GGATGGTCTGTGACTGTGAGAATCCCACAGTTGATCTTTTTTGCTGCCCTGAATGTGACCCAAGGCTTAG
  TAGTCAGTGCCTCCATCAAAATGGGGAAACTTTGTATAACAGTGGTGACACCTGGGTCCAGAATTGTCAA
  CAGTGCCGCTGCTTGCAAGGGGAAGTTGATTGTTGGCCCCTGCCTTGCCCAGATGTGGAGTGTGAATTCA
  GCATTCTCCCAGAGAATGAGTGCTGCCCGCGCTGTGTCACAGACCCTTGCCAGGCTGACACCATCCGCAA
  TGACATCACCAAGACTTGCCTGGACGAAATGAATGTGGTTCGCTTCACCGGGTCCTCTTGGATCAAACAT
  GGCACTGAGTGTACTCTCTGCCAGTGCAAGAATGGCCACATCTGTTGCTCAGTGGATCCACAGTGCCTTC
  AGGAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227443 representing NM_001145107
  Red=Cloning site Green=Tags(s)
  
  MGFPPLLKGQASATRSSLASCSWVVFFLSCLSRHAPEIEGGRRWTELIRTMESRVLLRTFCLIFGLGAVW
  GLGVDPSLQIDVLTELELGESTTGVRQVPGLHNGTKAFLFQDTPRSIKASTATAEQFFQKLRNKHEFTIL
  VTLKQTHLNSGVILSIHHLDHRYLELESSGHRNEVRLHYRSGSHRPHTEVFPYILADDKWHKLSLAISAS
  HLILHIDCNKIYERVVEKPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQLLVMPQGFIAQCPDLNRTCPT
  CNDFHGLVQKIMELQDILAKTSAKLSRAEQRMNRLDQCYCERTCTMKGTTYREFESWIDGCKNCTCLNGT
  IQCETLICPNPDCPLKSALAYVDGKCCKECKSICQFQGRTYFEGERNTVYSSSGVCVLYECKDQTMKLVE
  SSGCPALDCPESHQITLSHSCCKVCKGYDFCSERHNCMENSICRNLNDRAVCSCRDGFRALREDNAYCED
  IDECAEGRHYCRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNCVC
  KPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSDGFVQCDSRANCINLPGWYH
  CECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCIHDGKVKHNG
  QIWVLENDRCSVCSCQNGFVMCRRMVCDCENPTVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQ
  QCRCLQGEVDCWPLPCPDVECEFSILPENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKH
  GTECTLCQCKNGHICCSVDPQCLQEL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145107
• ORF Size: 2598 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145107.1, NP_001138579.1
• RefSeq Size: 3385
• RefSeq ORF: 2601
• Locus ID: 4753
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009]