EXOC7 (NM_001145297) Human Tagged ORF Clone

CAT#: RG227494

EXOC7 (GFP-tagged) - Human exocyst complex component 7 (EXOC7), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EXOC7
• Synonyms: 2-5-3p; BLOM4; EX070; EXO70; Exo70p; EXOC1; YJL085W
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227494 representing NM_001145297
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCCCCACAGGAGGCATCCGCTCGACGGCGGGAGATTGAGGACAAGCTGAAGCAGGAGGAGGAGA
  CTCTGTCCTTCATCCGAGACAGCCTGGAGAAGAGCGACCAGCTCACTAAGAACATGGTGTCTATCTTATC
  ATCCTTTGAGAGCCGCCTTATGAAGCTGGAGAACTCCATCATCCCTGTGCACAAGCAGACGGAGAATCTG
  CAGCGGCTGCAGGAGAATGTTGAGAAGACGCTGTCCTGCCTGGACCATGTCATCAGCTACTACCATGTGG
  CCAGTGACACTGAGAAGATCATCAGAGAGGGCCCCACAGGTAGGCTGGAAGAGTACCTGGGAAGCATGGC
  CAAGATTCAGAAGGCAGTGGAGTATTTCCAGGACAACAGCCCAGACAGCCCGGAACTCAACAAAGTGAAA
  CTGCTCTTTGAGCGCGGGAAGGAGGCCCTGGAGTCCGAATTTCGCAGCCTGATGACGCGGCACAGTAAGG
  TCGTCTCGCCCGTGCTCATCTTGGATCTGATCAGTGGTGACGATGATCTGGAGGCCCAGGAGGACGTGAC
  CCTGGAGCACCTGCCCGAGAGCGTGCTCCAGGATGTCATTCGCATCTCCCGCTGGCTGGTGGAATATGGC
  CGCAACCAAGATTTCATGAACGTCTACTACCAGATACGCTCCAGCCAGCTGGACCGCTCCATCAAAGGAC
  TGAAGGAGCATTTCCATAAGAGCAGTTCTTCCTCTGGGGTTCCCTACTCCCCTGCTATCCCCAACAAGAG
  GAAAGACACACCTACCAAGAAGCCAGTCAAGCGGCCAGGGACGATCCGTAAGGCTCAGAACCTTCTGAAA
  CAGTATTCCCAGCATGGTCTAGATGGGAAAAAGGGGGGCTCTAACCTCATTCCTCTGGAAGGGCTTCTTC
  CCTGTACCCCAAGGGGCGGCCTGCCTGGCCCCTGGATAAACGCTGCTTGTGTTTGTGCGGCCGACATCTC
  GCCAGGTCACGAGCATGATTTCCGAGTTAAGCACCTGTCCGAGGCCTTGAACGACAAGCACGGGCCGCTG
  GCCGGGAGAGATGACATGCTGGACGTGGAGACCGATGCCTACATCCACTGCGTCAGTGCCTTCGTCAAGC
  TGGCGCAGAGCGAGTACCAGCTGCTGGCCGACATCATCCCCGAGCACCACCAGAAGAAGACCTTCGACTC
  CCTGATACAGGATGCCCTGGATGGGCTGATGCTTGAAGGGGAGAACATCGTGTCTGCTGCCCGGAAGGCC
  ATTGTGCGACACGACTTCTCCACGGTGCTCACCGTCTTCCCCATCCTGCGACACCTCAAGCAGACCAAGC
  CTGAGTTTGACCAGGTGCTCCAGGGCACGGCTGCCAGCACAAAGAACAAGCTGCCTGGCCTCATCACATC
  CATGGAGACCATCGGTGCCAAAGCGCTGGAGGACTTCGCAGACAACATCAAGAATGACCCGGACAAGGAG
  TACAACATGCCGAAGGACGGCACCGTACACGAGCTCACCAGCAATGCCATCCTCTTCCTGCAGCAGCTTT
  TGGACTTCCAGGAGACGGCAGGCGCCATGCTGGCCTCCCAAGAGACCAGCTCTTCGGCCACCAGCTACAG
  CTCTGAGTTCAGCAAGCGGCTGCTAAGCACCTATATCTGTAAAGTGCTGGGCAACCTGCAGTTGAACTTG
  CTGAGCAAGTCCAAGGTGTACGAGGACCCAGCTCTGAGCGCCATCTTCCTGCACAACAACTACAATTACA
  TCCTCAAGTCCCTGGAGAAGTCTGAACTGATCCAGCTGGTGGCAGTGACACAGAAGACTGCTGAGCGCTC
  CTACCGGGAGCACATTGAGCAGCAGATCCAGACCTACCAGCGCAGCTGGTTAAAGGTGACTGATTACATC
  GCAGAGAAGAATCTACCTGTGTTCCAGCCGGGAGTCAAGCTCCGGGACAAGGAGCGGCAGATTATCAAGG
  AGCGTTTTAAGGGCTTCAATGATGGCCTCGAAGAACTGTGCAAAATCCAGAAGGCCTGGGCTATTCCAGA
  CACAGAGCAGAGGGACAGGATTCGCCAGGCCCAGAAGACCATTGTCAAGGAGACCTACGGGGCCTTTCTA
  CAGAAGTTTGGCAGCGTGCCCTTCACCAAGAACCCGGAGAAGTACATCAAGTACGGGGTGGAGCAGGTGG
  GCGACATGATCGATCGCCTTTTCGACACCTCTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227494 representing NM_001145297
  Red=Cloning site Green=Tags(s)
  
  MIPPQEASARRREIEDKLKQEEETLSFIRDSLEKSDQLTKNMVSILSSFESRLMKLENSIIPVHKQTENL
  QRLQENVEKTLSCLDHVISYYHVASDTEKIIREGPTGRLEEYLGSMAKIQKAVEYFQDNSPDSPELNKVK
  LLFERGKEALESEFRSLMTRHSKVVSPVLILDLISGDDDLEAQEDVTLEHLPESVLQDVIRISRWLVEYG
  RNQDFMNVYYQIRSSQLDRSIKGLKEHFHKSSSSSGVPYSPAIPNKRKDTPTKKPVKRPGTIRKAQNLLK
  QYSQHGLDGKKGGSNLIPLEGLLPCTPRGGLPGPWINAACVCAADISPGHEHDFRVKHLSEALNDKHGPL
  AGRDDMLDVETDAYIHCVSAFVKLAQSEYQLLADIIPEHHQKKTFDSLIQDALDGLMLEGENIVSAARKA
  IVRHDFSTVLTVFPILRHLKQTKPEFDQVLQGTAASTKNKLPGLITSMETIGAKALEDFADNIKNDPDKE
  YNMPKDGTVHELTSNAILFLQQLLDFQETAGAMLASQETSSSATSYSSEFSKRLLSTYICKVLGNLQLNL
  LSKSKVYEDPALSAIFLHNNYNYILKSLEKSELIQLVAVTQKTAERSYREHIEQQIQTYQRSWLKVTDYI
  AEKNLPVFQPGVKLRDKERQIIKERFKGFNDGLEELCKIQKAWAIPDTEQRDRIRQAQKTIVKETYGAFL
  QKFGSVPFTKNPEKYIKYGVEQVGDMIDRLFDTSA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145297
• ORF Size: 2205 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145297.2, NP_001138769.1
• RefSeq Size: 4952
• RefSeq ORF: 2208
• Locus ID: 23265
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway
• Gene Summary: The protein encoded by this gene is a component of the exocyst complex. The exocyst complex plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. The encoded protein is required for assembly of the exocyst complex and docking of the complex to the plasma membrane. The encoded protein may also play a role in pre-mRNA splicing through interactions with pre-mRNA-processing factor 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Nov 2011]