NELL2 (NM_001145108) Human Tagged ORF Clone

CAT#: RG227592

NELL2 (GFP-tagged) - Human NEL-like 2 (chicken) (NELL2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NELL2
• Synonyms: NRP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227592 representing NM_001145108
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCTCGGGTCTTACTGAGAACATTCTGTTTGATCTTCGGTCTCGGAGCAGTTTGGGGGCTTGGTG
  TGGACCCTTCCCTACAGATTGACGTCTTAACAGAGTTAGAACTTGGGGAGTCCACGACCGGAGTGCGTCA
  GGTCCCGGGGCTGCATAATGGGACGAAAGCCTTTCTCTTTCAAGATACTCCCAGAAGCATAAAAGCATCC
  ACTGCTACAGCTGAACAGTTTTTTCAGAAGCTGAGAAATAAACATGAATTTACTATTTTGGTGACCCTAA
  AACAGACCCACTTAAATTCAGGAGTTATTCTCTCAATTCACCACTTGGATCACAGGTACCTGGAACTGGA
  AAGTAGTGGCCATCGGAATGAAGTCAGACTGCATTACCGCTCAGGCAGTCACCGCCCTCACACAGAAGTG
  TTTCCTTACATTTTGGCTGATGACAAGTGGCACAAGCTCTCCTTAGCCATCAGTGCTTCCCATTTGATTT
  TACACATTGACTGCAATAAAATTTATGAAAGGGTAGTAGAAAAGCCCTCCACAGACTTGCCTCTAGGCAC
  AACATTTTGGCTAGGACAGAGAAATAATGCGCATGGATATTTTAAGGGTATAATGCAAGATGTCCAATTA
  CTTGTCATGCCCCAGGGATTTATTGCTCAGTGCCCAGATCTTAATCGCACCTGTCCAACTTGCAATGACT
  TCCATGGACTTGTGCAGAAAATCATGGAGCTACAGGATATTTTAGCCAAAACATCAGCCAAGCTGTCTCG
  AGCTGAACAGCGAATGAATAGATTGGATCAGTGCTATTGTGAAAGGACTTGCACCATGAAGGGAACCACC
  TACCGAGAATTTGAGTCCTGGATAGACGGCTGTAAGAACTGCACATGCCTGAATGGAACCATCCAGTGTG
  AAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGGCTCTTGCGTATGTGGATGGCAAATGCTG
  TAAGGAATGCAAATCGATATGCCAATTTCAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTAT
  TCCTCTTCTGGAGTATGTGTTCTCTATGAGTGCAAGGACCAGACCATGAAACTTGTTGAGAGTTCAGGCT
  GTCCAGCTTTGGATTGTCCAGAGTCTCATCAGATAACCTTGTCTCACAGCTGTTGCAAAGTTTGTAAAGG
  TTATGACTTTTGTTCTGAAAGGCATAACTGCATGGAGAATTCCATCTGCAGAAATCTGAATGACAGGGCT
  GTTTGTAGCTGTCGAGATGGTTTTAGGGCTCTTCGAGAGGATAATGCCTACTGTGAAGACATCGATGAGT
  GTGCTGAAGGGCGCCATTACTGTCGTGAAAATACAATGTGTGTCAACACCCCGGGTTCTTTTATGTGCAT
  CTGCAAAACTGGATACATCAGAATTGATGATTATTCATGTACAGAACATGATGAGTGTATCACAAATCAG
  CACAACTGTGATGAAAATGCTTTATGCTTCAACACTGTTGGAGGACACAACTGTGTTTGCAAGCCGGGCT
  ATACAGGGAATGGAACGACATGCAAAGCATTTTGCAAAGATGGCTGTAGGAATGGAGGAGCCTGTATTGC
  CGCTAATGTGTGTGCCTGCCCACAAGGCTTCACTGGACCCAGCTGTGAAACGGACATTGATGAATGCTCT
  GATGGTTTTGTTCAATGTGACAGTCGTGCTAATTGCATTAACCTGCCTGGATGGTACCACTGTGAGTGCA
  GAGATGGCTACCATGACAATGGGATGTTTTCACCAAGTGGAGAATCGTGTGAAGATATTGATGAGTGTGG
  GACCGGGAGGCACAGCTGTGCCAATGATACCATTTGCTTCAATTTGGATGGCGGATATGATTGTCGATGT
  CCTCATGGAAAGAATTGCACAGGGGACTGCATCCATGATGGAAAAGTTAAGCACAATGGTCAGATTTGGG
  TGTTGGAAAATGACAGGTGCTCTGTGTGCTCATGTCAGAATGGATTCGTTATGTGTCGACGGATGGTCTG
  TGACTGTGAGAATCCCACAGTTGATCTTTTTTGCTGCCCTGAATGTGACCCAAGGCTTAGTAGTCAGTGC
  CTCCATCAAAATGGGGAAACTTTGTATAACAGTGGTGACACCTGGGTCCAGAATTGTCAACAGTGCCGCT
  GCTTGCAAGGGGAAGTTGATTGTTGGCCCCTGCCTTGCCCAGATGTGGAGTGTGAATTCAGCATTCTCCC
  AGAGAATGAGTGCTGCCCGCGCTGTGTCACAGACCCTTGCCAGGCTGACACCATCCGCAATGACATCACC
  AAGACTTGCCTGGACGAAATGAATGTGGTTCGCTTCACCGGGTCCTCTTGGATCAAACATGGCACTGAGT
  GTACTCTCTGCCAGTGCAAGAATGGCCACATCTGTTGCTCAGTGGATCCACAGTGCCTTCAGGAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227592 representing NM_001145108
  Red=Cloning site Green=Tags(s)
  
  MESRVLLRTFCLIFGLGAVWGLGVDPSLQIDVLTELELGESTTGVRQVPGLHNGTKAFLFQDTPRSIKAS
  TATAEQFFQKLRNKHEFTILVTLKQTHLNSGVILSIHHLDHRYLELESSGHRNEVRLHYRSGSHRPHTEV
  FPYILADDKWHKLSLAISASHLILHIDCNKIYERVVEKPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQL
  LVMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILAKTSAKLSRAEQRMNRLDQCYCERTCTMKGTT
  YREFESWIDGCKNCTCLNGTIQCETLICPNPDCPLKSALAYVDGKCCKECKSICQFQGRTYFEGERNTVY
  SSSGVCVLYECKDQTMKLVESSGCPALDCPESHQITLSHSCCKVCKGYDFCSERHNCMENSICRNLNDRA
  VCSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQ
  HNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECS
  DGFVQCDSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDGGYDCRC
  PHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVCDCENPTVDLFCCPECDPRLSSQC
  LHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVECEFSILPENECCPRCVTDPCQADTIRNDIT
  KTCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHICCSVDPQCLQEL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145108
• ORF Size: 2448 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145108.1, NP_001138580.1
• RefSeq Size: 3705
• RefSeq ORF: 2451
• Locus ID: 4753
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009]