SEC61A2 (NM_001142628) Human Tagged ORF Clone

CAT#: RG227681

SEC61A2 (GFP-tagged) - Human Sec61 alpha 2 subunit (S. cerevisiae) (SEC61A2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SEC61A2
• Synonyms: FLJ10578
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227681 representing NM_001142628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCAAATTTTTAGAAGTTATCAAACCATTCTGTGCAGTTCTACCAGAAATTCAGAAACCGGAAA
  GGAAAATCCCACTGTTTGGAATCATGTCATCAGATTCTGCAGATCCTTTCTACTGGATGAGAGTTATTCT
  GGCTTCCAATAGAGGAACTTTAATGGAATTGGGTATCTCCCCAATTGTAACATCTGGTTTGATTATGCAG
  TTGTTAGCTGGAGCCAAAATCATTGAAGTTGGAGATACACCGAAAGATAGAGCTTTATTCAATGGAGCCC
  AGAAACTGTTTGGTATGATCATTACCATTGGGCAAGCCATTGTGTATGTCATGACGGGGATGTATGGGGA
  CCCTGCAGAAATGGGTGCCGGAATCTGTCTCCTGATCATCATTCAGTTGTTTGTTGCTGGTTTGATTGTG
  CTGCTGTTAGATGAGCTGCTACAGAAGGGTTACGGCTTGGGGTCTGGGATTTCCCTCTTTATTGCCACCA
  ACATCTGTGAGACCATTGTCTGGAAGGCCTTTAGTCCCACTACCATTAACACTGGCAGAGGTACTGAGTT
  TGAGGGTGCAGTCATAGCTCTGTTCCATTTGTTGGCCACCAGGACGGACAAAGTCCGAGCTTTACGGGAG
  GCTTTTTATCGGCAGAACTTACCCAATCTCATGAACCTCATTGCTACAGTTTTTGTGTTTGCTGTTGTTA
  TATATTTCCAAGGATTTCGCGTTGATCTGCCCATTAAGTCGGCCCGTTACCGAGGACAGTACAGCAGCTA
  CCCCATCAAACTCTTCTACACCTCCAACATCCCCATCATCCTCCAGTCGGCCCTGGTGTCCAACCTGTAT
  GTTATTTCCCAGATGCTGTCTGTTCGATTTAGTGGCAACTTTTTAGTAAATTTACTAGGACAGTGGGCCG
  ATGTCAGTGGGGGAGGACCCGCACGTTCTTACCCAGTTGGAGGCCTTTGTTACTATCTTTCTCCTCCTGA
  GTCCATGGGCGCCATCTTTGAGGATCCTGTCCATGTCGTTGTTTATATCATCTTCATGTTGGGGTCATGT
  GCATTCTTCTCTAAGACATGGATTGAAGTGTCTGGTTCCTCAGCCAAAGATGTAGCTAAACAGCTGAAAG
  AACAGCAGATGGTAATGAGGGGCCACCGAGATACCTCTATGGTTCATGAGCTTAATAGGTACATCCCCAC
  CGCAGCTGCGTTTGGCGGTTTGTGCATTGGCGCCCTGTCAGTGCTGGCTGACTTCCTGGGGGCCATTGGA
  TCTGGCACTGGAATTCTGCTAGCAGTCACTATTATTTACCAGTATTTTGAAATATTTGTTAAAGAACAGG
  CCGAAGTTGGTGGGATGGGTGCTTTGTTTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227681 representing NM_001142628
  Red=Cloning site Green=Tags(s)
  
  MGIKFLEVIKPFCAVLPEIQKPERKIPLFGIMSSDSADPFYWMRVILASNRGTLMELGISPIVTSGLIMQ
  LLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIV
  LLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALRE
  AFYRQNLPNLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSNLY
  VISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVHVVVYIIFMLGSC
  AFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIG
  SGTGILLAVTIIYQYFEIFVKEQAEVGGMGALFF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142628
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142628.1, NP_001136100.1
• RefSeq Size: 2462
• RefSeq ORF: 1365
• Locus ID: 55176
• Protein Families: Transmembrane
• Protein Pathways: Vibrio cholerae infection
• Gene Summary: The protein encoded by this gene has similarity to a mouse protein which suggests a role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. It may also be required for the assembly of membrane and secretory proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]