PPP3CB (NM_001142354) Human Tagged ORF Clone

CAT#: RG227763

PPP3CB (GFP-tagged) - Human protein phosphatase 3, catalytic subunit, beta isozyme (PPP3CB), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPP3CB
• Synonyms: CALNA2; CALNB; CNA2; PP2Bbeta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227763 representing NM_001142354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCCCGGAGCCGGCCCGGGCTGCACCGCCCCCACCCCCGCCCCCGCCGCCCCCTCCCGGGGCTG
  ACCGCGTCGTCAAAGCTGTCCCTTTCCCCCCAACACATCGCTTGACATCTGAAGAAGTATTTGATTTGGA
  TGGGATACCCAGGGTTGATGTTCTGAAGAACCACTTGGTGAAAGAAGGTCGAGTAGATGAAGAAATTGCG
  CTTAGAATTATCAATGAGGGTGCTGCCATCCTTCGGAGAGAGAAAACCATGATAGAAGTAGAAGCTCCAA
  TCACAGTGTGTGGTGACATCCATGGCCAATTTTTTGATCTGATGAAACTTTTTGAAGTAGGAGGATCACC
  TGCTAATACACGATACCTTTTTCTTGGCGATTATGTGGACAGAGGTTATTTTAGTATAGAGTGTGTCTTA
  TATTTATGGGTTCTGAAGATTCTATACCCAAGCACATTATTTCTTCTGAGAGGCAACCATGAATGCAGAC
  ACCTTACTGAATATTTTACCTTTAAGCAGGAATGTAAAATTAAGTATTCGGAAAGAGTCTATGAAGCTTG
  TATGGAAGCTTTTGATAGTTTGCCTCTTGCTGCACTTTTAAACCAACAGTTTCTTTGTGTTCATGGTGGA
  CTTTCACCAGAAATACACACACTGGATGATATTAGGAGATTAGATAGATTCAAAGAGCCACCTGCATTTG
  GACCAATGTGTGACTTGTTATGGTCCGATCCTTCTGAAGATTTTGGAAATGAAAAATCACAGGAACATTT
  TAGTCACAATACAGTTCGAGGATGTTCTTATTTTTATAACTATCCAGCAGTGTGTGAATTTTTGCAAAAC
  AATAATTTGTTATCGATTATTAGAGCTCATGAAGCTCAAGATGCAGGCTATAGAATGTACAGAAAAAGTC
  AAACTACAGGGTTCCCTTCATTAATAACAATTTTTTCGGCACCTAATTACTTAGATGTCTACAATAATAA
  AGCTGCTGTATTAAAGTATGAAAATAATGTGATGAATATTCGACAGTTTAACTGTTCTCCACATCCTTAC
  TGGTTGCCTAATTTTATGGATGTCTTCACGTGGTCTTTACCGTTTGTTGGAGAAAAAGTGACAGAAATGT
  TGGTAAATGTTCTGAGTATTTGCTCTGATGATGAACTAATGACTGAAGGTGAAGACCAGTTTGATGTAGG
  TTCAGCTGCAGCCCGGAAAGAAATCATAAGAAACAAAATTCGAGCAATTGGCAAGATGGCAAGAGTCTTC
  TCTGTTCTCAGGGAGGAGAGTGAAAGTGTGCTGACACTCAAGGGCCTGACTCCCACAGGGATGTTGCCTA
  GTGGAGTGTTAGCTGGAGGACGGCAGACCCTGCAAAGTGCAATACGAGGATTCTCTCCACCACATAGAAT
  CTGCAGTTTTGAAGAGGCAAAGGGTTTGGATAGGATCAATGAGAGAATGCCACCTCGGAAAGATGCTGTA
  CAGCAAGATGGTTTCAATTCTCTGAACACCGCACATGCCACTGAGAACCACGGGACGGGCAACCATACTG
  CCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227763 representing NM_001142354
  Red=Cloning site Green=Tags(s)
  
  MAAPEPARAAPPPPPPPPPPPGADRVVKAVPFPPTHRLTSEEVFDLDGIPRVDVLKNHLVKEGRVDEEIA
  LRIINEGAAILRREKTMIEVEAPITVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVL
  YLWVLKILYPSTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYEACMEAFDSLPLAALLNQQFLCVHGG
  LSPEIHTLDDIRRLDRFKEPPAFGPMCDLLWSDPSEDFGNEKSQEHFSHNTVRGCSYFYNYPAVCEFLQN
  NNLLSIIRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPY
  WLPNFMDVFTWSLPFVGEKVTEMLVNVLSICSDDELMTEGEDQFDVGSAAARKEIIRNKIRAIGKMARVF
  SVLREESESVLTLKGLTPTGMLPSGVLAGGRQTLQSAIRGFSPPHRICSFEEAKGLDRINERMPPRKDAV
  QQDGFNSLNTAHATENHGTGNHTAQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142354
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142354.1, NP_001135826.1
• RefSeq Size: 3137 bp
• RefSeq ORF: 1548 bp
• Locus ID: 5532
• Cytogenetics: 10q22.2
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: ''