NELL2 (NM_001145110) Human Tagged ORF Clone

CAT#: RG227981

NELL2 (GFP-tagged) - Human NEL-like 2 (chicken) (NELL2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NELL2
• Synonyms: NRP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227981 representing NM_001145110
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTATAAGAAGACTTCTCATTTTAATCCTCAAAATAGGGAGACGATGGACTGAGCTGATCCGCACCA
  TGGAGTCTCGGGTCTTACTGAGAACATTCTGTTTGATCTTCGGTCTCGGAGCAGTTTGGGGGCTTGGTGT
  GGACCCTTCCCTACAGATTGACGTCTTAACAGAGTTAGAACTTGGGGAGTCCACGACCGGAGTGCGTCAG
  GTCCCGGGGCTGCATAATGGGACGAAAGCCTTTCTCTTTCAAGATACTCCCAGAAGCATAAAAGCATCCA
  CTGCTACAGCTGAACAGTTTTTTCAGAAGCTGAGAAATAAACATGAATTTACTATTTTGGTGACCCTAAA
  ACAGACCCACTTAAATTCAGGAGTTATTCTCTCAATTCACCACTTGGATCACAGGTACCTGGAACTGGAA
  AGTAGTGGCCATCGGAATGAAGTCAGACTGCATTACCGCTCAGGCAGTCACCGCCCTCACACAGAAGTGT
  TTCCTTACATTTTGGCTGATGACAAGTGGCACAAGCTCTCCTTAGCCATCAGTGCTTCCCATTTGATTTT
  ACACATTGACTGCAATAAAATTTATGAAAGGGTAGTAGAAAAGCCCTCCACAGACTTGCCTCTAGGCACA
  ACATTTTGGCTAGGACAGAGAAATAATGCGCATGGATATTTTAAGGGTATAATGCAAGATGTCCAATTAC
  TTGTCATGCCCCAGGGATTTATTGCTCAGTGCCCAGATCTTAATCGCACCTGTCCAACTTGCAATGACTT
  CCATGGACTTGTGCAGAAAATCATGGAGCTACAGGATATTTTAGCCAAAACATCAGCCAAGCTGTCTCGA
  GCTGAACAGCGAATGAATAGATTGGATCAGTGCTATTGTGAAAGGACTTGCACCATGAAGGGAACCACCT
  ACCGAGAATTTGAGTCCTGGATAGACGGCTGTAAGAACTGCACATGCCTGAATGGAACCATCCAGTGTGA
  AACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGGCTCTTGCGTATGTGGATGGCAAATGCTGT
  AAGGAATGCAAATCGATATGCCAATTTCAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATT
  CCTCTTCTGGAGTATGTGTTCTCTATGAGTGCAAGGACCAGACCATGAAACTTGTTGAGAGTTCAGGCTG
  TCCAGCTTTGGATTGTCCAGAGTCTCATCAGATAACCTTGTCTCACAGCTGTTGCAAAGTTTGTAAAGGT
  TATGACTTTTGTTCTGAAAGGCATAACTGCATGGAGAATTCCATCTGCAGAAATCTGAATGACAGGGCTG
  TTTGTAGCTGTCGAGATGGTTTTAGGGCTCTTCGAGAGGATAATGCCTACTGTGAAGACATCGATGAGTG
  TGCTGAAGGGCGCCATTACTGTCGTGAAAATACAATGTGTGTCAACACCCCGGGTTCTTTTATGTGCATC
  TGCAAAACTGGATACATCAGAATTGATGATTATTCATGTACAGAACATGATGAGTGTATCACAAATCAGC
  ACAACTGTGATGAAAATGCTTTATGCTTCAACACTGTTGGAGGACACAACTGTGTTTGCAAGCCGGGCTA
  TACAGGGAATGGAACGACATGCAAAGCATTTTGCAAAGATGGCTGTAGGAATGGAGGAGCCTGTATTGCC
  GCTAATGTGTGTGCCTGCCCACAAGGCTTCACTGGACCCAGCTGTGAAACGGACATTGATGAATGCTCTG
  ATGGTTTTGTTCAATGTGACAGTCGTGCTAATTGCATTAACCTGCCTGGATGGTACCACTGTGAGTGCAG
  AGATGGCTACCATGACAATGGGATGTTTTCACCAAGTGGAGAATCGTGTGAAGATATTGATGAGTGTGGG
  ACCGGGAGGCACAGCTGTGCCAATGATACCATTTGCTTCAATTTGGATGGCGGATATGATTGTCGATGTC
  CTCATGGAAAGAATTGCACAGGGGACTGCATCCATGATGGAAAAGTTAAGCACAATGGTCAGATTTGGGT
  GTTGGAAAATGACAGGTGCTCTGTGTGCTCATGTCAGAATGGATTCGTTATGTGTCGACGGATGGTCTGT
  GACTGTGAGAATCCCACAGTTGATCTTTTTTGCTGCCCTGAATGTGACCCAAGGCTTAGTAGTCAGTGCC
  TCCATCAAAATGGGGAAACTTTGTATAACAGTGGTGACACCTGGGTCCAGAATTGTCAACAGTGCCGCTG
  CTTGCAAGGGGAAGTTGATTGTTGGCCCCTGCCTTGCCCAGATGTGGAGTGTGAATTCAGCATTCTCCCA
  GAGAATGAGTGCTGCCCGCGCTGTGTCACAGACCCTTGCCAGGCTGACACCATCCGCAATGACATCACCA
  AGACTTGCCTGGACGAAATGAATGTGGTTCGCTTCACCGGGTCCTCTTGGATCAAACATGGCACTGAGTG
  TACTCTCTGCCAGTGCAAGAATGGCCACATCTGTTGCTCAGTGGATCCACAGTGCCTTCAGGAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227981 representing NM_001145110
  Red=Cloning site Green=Tags(s)
  
  MSIRRLLILILKIGRRWTELIRTMESRVLLRTFCLIFGLGAVWGLGVDPSLQIDVLTELELGESTTGVRQ
  VPGLHNGTKAFLFQDTPRSIKASTATAEQFFQKLRNKHEFTILVTLKQTHLNSGVILSIHHLDHRYLELE
  SSGHRNEVRLHYRSGSHRPHTEVFPYILADDKWHKLSLAISASHLILHIDCNKIYERVVEKPSTDLPLGT
  TFWLGQRNNAHGYFKGIMQDVQLLVMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILAKTSAKLSR
  AEQRMNRLDQCYCERTCTMKGTTYREFESWIDGCKNCTCLNGTIQCETLICPNPDCPLKSALAYVDGKCC
  KECKSICQFQGRTYFEGERNTVYSSSGVCVLYECKDQTMKLVESSGCPALDCPESHQITLSHSCCKVCKG
  YDFCSERHNCMENSICRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFMCI
  CKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIA
  ANVCACPQGFTGPSCETDIDECSDGFVQCDSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECG
  TGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVC
  DCENPTVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVECEFSILP
  ENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHICCSVDPQCLQEL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145110
• ORF Size: 2517 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145110.1, NP_001138582.1
• RefSeq Size: 3271
• RefSeq ORF: 2520
• Locus ID: 4753
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009]