CLK1 (NM_001162407) Human Tagged ORF Clone

CAT#: RG228428

CLK1 (GFP-tagged) - Human CDC-like kinase 1 (CLK1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CLK1
• Synonyms: CLK; CLK/STY; STY
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228428 representing NM_001162407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGGGCGGAGGCCGGCTTCGGCCCTGTGGCCGGAAAGGCGAGGCTCCCCGTTGAGGGGGGATT
  TGCTGGGGTTCCAGAATGTGCGTGAGCCAAGCAGCTGTGGGGAAACGTTGTCTGGAATGAGACACTCAAA
  GAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGAAAATGGAGGAGCAGCAGCAGTCAT
  AAAAGAAGGAAGAGATCACATAGCAGTGCCCAGGAGAACAAGCGCTGCAAATACAATCACTCTAAAATGT
  GTGATAGCCATTATTTGGAAAGCAGGTCTATAAATGAGAAAGATTATCATAGTCGACGCTACATTGATGA
  GTACAGAAATGACTACACTCAAGGATGTGAACCTGGACATCGCCAAAGAGACCATGAAAGCCGGTATCAG
  AACCATAGTAGCAAGTCTTCTGGTAGAAGTGGAAGAAGTAGTTATAAAAGCAAACACAGGATTCACCACA
  GTACTTCACATCGTCGTTCACATGGGAAGAGTCACCGAAGGAAAAGAACCAGGAGTGTAGAGGATGATGA
  GGAGGGTCACCTGATCTGTCAGAGTGGAGACGTACTAAGTGCAAGATATGAAATTGTTGATACTTTAGGT
  GAAGGAGCTTTTGGAAAAGTTGTGGAGTGCATCGATCATAAAGCGGGAGGTAGACATGTAGCAGTAAAAA
  TAGTTAAAAATGTGGATAGATACTGTGAAGCTGCTCGCTCAGAAATACAAGTTCTGGAACATCTGAATAC
  AACAGACCCCAACAGTACTTTCCGCTGTGTCCAGATGTTGGAATGGTTTGAGCATCATGGTCACATTTGC
  ATTGTTTTTGAACTATTGGGACTTAGTACTTACGACTTCATTAAAGAAAATGGTTTTCTACCATTTCGAC
  TGGATCATATCAGAAAGATGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCACAGTAATAAGTTGAC
  TCACACAGACTTAAAGCCTGAAAACATCTTATTTGTGCAGTCTGACTACACAGAGGCGTATAATCCCAAA
  ATAAAACGTGATGAACGCACCTTAATAAATCCAGATATTAAAGTTGTAGACTTTGGTAGTGCAACATATG
  ATGACGAACATCACAGTACATTGGTATCTACAAGACATTATAGAGCACCTGAAGTTATTTTAGCCCTAGG
  GTGGTCCCAACCATGTGATGTCTGGAGCATAGGATGCATTCTTATTGAATACTATCTTGGGTTTACCGTA
  TTTCCAACACACGATAGTAAGGAGCATTTAGCAATGATGGAAAGGATTCTTGGACCTCTACCAAAACATA
  TGATACAGAAAACCAGGAAACGTAAATATTTTCACCACGATCGATTAGACTGGGATGAACACAGTTCTGC
  CGGCAGATATGTTTCAAGACGCTGTAAACCTCTGAAGGAATTTATGCTTTCTCAAGATGTTGAACATGAG
  CGTCTCTTTGACCTCATTCAGAAAATGTTGGAGTATGATCCAGCCAAAAGAATTACTCTCAGAGAAGCCT
  TAAAGCATCCTTTCTTTGACCTTCTGAAGAAAAGTATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228428 representing NM_001162407
  Red=Cloning site Green=Tags(s)
  
  MAAGRRPASALWPERRGSPLRGDLLGFQNVREPSSCGETLSGMRHSKRTYCPDWDDKDWDYGKWRSSSSH
  KRRKRSHSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSRRYIDEYRNDYTQGCEPGHRQRDHESRYQ
  NHSSKSSGRSGRSSYKSKHRIHHSTSHRRSHGKSHRRKRTRSVEDDEEGHLICQSGDVLSARYEIVDTLG
  EGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHIC
  IVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPK
  IKRDERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTV
  FPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDVEHE
  RLFDLIQKMLEYDPAKRITLREALKHPFFDLLKKSI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162407
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001162407.1, NP_001155879.1
• RefSeq Size: 2213
• RefSeq ORF: 1581
• Locus ID: 1195
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]