PPP2R5C (NM_001161725) Human Tagged ORF Clone

CAT#: RG228446

PPP2R5C (GFP-tagged) - Human protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPP2R5C
• Synonyms: B56G; B56gamma; PR61G
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228446 representing NM_001161725
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAATAAAAACAAGAAGGAGAAAGAATCACCAAAAGCAGGGAAGAGTGGAAAAAGTTCAAAAGAAG
  GACAAGACACAGTAGAATCAGAGGGAACAAGTCCTGAAGAACCCTCAAGCCCTAAAGTACCACCTCCACT
  TCTTCCTGAGTTGCTGGTTCTTATTTTTGGTGGGCTTCAAGGAAGAGATGTTCCTCCTGCTGATCAAGAG
  AAGCTTTTTATCCAGAAGTTACGTCAGTGTTGCGTCCTCTTTGACTTTGTTTCTGATCCACTAAGTGACC
  TAAAGTGGAAGGAAGTAAAACGAGCTGCTTTAAGTGAAATGGTAGAATATATCACCCATAATCGGAATGT
  GATCACAGAGCCTATTTACCCAGAAGTAGTCCATATGTTTGCAGTTAACATGTTTCGAACATTACCACCT
  TCCTCCAATCCTACGGGAGCGGAATTTGACCCGGAGGAAGATGAACCAACGTTAGAAGCAGCCTGGCCTC
  ATCTACAGCTTGTTTATGAATTTTTCTTAAGATTTTTAGAGTCTCCAGATTTCCAACCTAATATAGCGAA
  GAAATATATTGATCAGAAGTTTGTATTGCAGCTTTTAGAGCTCTTTGACAGTGAAGATCCTCGGGAGAGA
  GATTTTCTTAAAACCACCCTTCACAGAATCTATGGGAAATTCCTAGGCTTGAGAGCTTACATCAGAAAAC
  AGATAAATAATATATTTTATAGGTTTATTTATGAAACAGAGCATCATAATGGCATAGCAGAGTTACTGGA
  AATATTGGGAAGTATAATTAATGGATTTGCCTTACCACTAAAAGAAGAGCACAAGATTTTCTTATTGAAG
  GTGTTACTACCTTTGCACAAAGTGAAATCTCTGAGTGTCTACCATCCCCAGCTGGCATACTGTGTAGTGC
  AGTTTTTAGAAAAGGACAGCACCCTCACGGAACCAGTGGTGATGGCACTTCTCAAATACTGGCCAAAGAC
  TCACAGTCCAAAAGAAGTAATGTTCTTAAACGAATTAGAAGAGATTTTAGATGTCATTGAACCATCAGAA
  TTTGTGAAGATCATGGAACCCCTCTTCCGGCAGTTGGCCAAATGTGTCTCCAGCCCACACTTCCAGGTGG
  CAGAGCGAGCTCTCTATTACTGGAATAATGAATACATCATGAGTTTAATCAGTGACAACGCAGCGAAGAT
  TCTGCCCATCATGTTTCCTTCCTTGTACCGCAACTCAAAGACCCATTGGAACAAGACAATACATGGCTTG
  ATATACAACGCCCTGAAGCTCTTCATGGAGATGAACCAAAAGCTATTTGATGACTGTACACAACAGTTCA
  AAGCAGAGAAACTAAAAGAGAAGCTAAAAATGAAAGAACGGGAAGAAGCATGGGTTAAAATAGAAAATCT
  AGCCAAAGCCAATCCCCAGTACACAGTGTATAGTCAAGCCAGCACCATGAGCATTCCGGTTGCAATGGAG
  ACAGATGGGCCTTTATTTGAAGATGTGCAGATGCTGAGAAAGACAGTGAAGGACGAGGCTCATCAGGCAC
  AGAAAGATCCGAAGAAGGACCGTCCTCTTGCACGCCGCAAGTCCGAGCTGCCTCAGGACCCCCACACCAA
  GAAAGCCTTGGAAGCTCACTGCAGGGCCGATGAGCTGGCCTCCCAGGACGGCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228446 representing NM_001161725
  Red=Cloning site Green=Tags(s)
  
  MPNKNKKEKESPKAGKSGKSSKEGQDTVESEGTSPEEPSSPKVPPPLLPELLVLIFGGLQGRDVPPADQE
  KLFIQKLRQCCVLFDFVSDPLSDLKWKEVKRAALSEMVEYITHNRNVITEPIYPEVVHMFAVNMFRTLPP
  SSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLQLLELFDSEDPRER
  DFLKTTLHRIYGKFLGLRAYIRKQINNIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLK
  VLLPLHKVKSLSVYHPQLAYCVVQFLEKDSTLTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPSE
  FVKIMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAAKILPIMFPSLYRNSKTHWNKTIHGL
  IYNALKLFMEMNQKLFDDCTQQFKAEKLKEKLKMKEREEAWVKIENLAKANPQYTVYSQASTMSIPVAME
  TDGPLFEDVQMLRKTVKDEAHQAQKDPKKDRPLARRKSELPQDPHTKKALEAHCRADELASQDGR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161725
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161725.1, NP_001155197.1
• RefSeq Size: 4494 bp
• RefSeq ORF: 1668 bp
• Locus ID: 5527
• Cytogenetics: 14q32.31
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• Gene Summary: 'The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'