Acinus (ACIN1) (NM_001164817) Human Tagged ORF Clone

CAT#: RG228475

ACIN1 (GFP-tagged) - Human apoptotic chromatin condensation inducer 1 (ACIN1), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ACIN1
• Synonyms: ACINUS; ACN; fSAP152
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228475 representing NM_001164817
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTATCAGAAAGCAAAGAAGGTGAGGAGAAGGAGGAAGTGACCATGGACACAAGTGAAAACAGACCTG
  AAAATGATGTTCCAGAACCTCCCATGCCTATTGCAGACCAAGTCAGCAATGATGACCGCCCGGAGGGCAG
  TGTTGAAGATGAGGAGAAGAAAGAGAGCTCGCTGCCCAAATCATTCAAGAGGAAGATCTCCGTTGTCTCA
  GCTACCAAGGGGGTGCCAGCTGGAAACAGTGACACAGAGGGGGGCCAGCCTGGTCGGAAACGACGCTGGG
  GAGCCAGCACAGCCACCACACAGAAGAAACCTTCCATCAGTATCACCACTGAATCACTAAAGAGCCTCAT
  CCCCGACATCAAACCCCTGGCGGGGCAGGAGGCTGTTGTGGATCTTCATGCTGATGACTCTCGCATCTCT
  GAGGATGAGACAGAGCGTAATGGCGATGATGGGACCCATGACAAGGGGCTGAAAATATGCCGGACAGTCA
  CTCAGGTAGTACCTGCAGAGGGCCAGGAGAATGGGCAGAGGGAAGAAGAGGAAGAAGAGAAGGAACCTGA
  AGCAGAACCTCCTGTACCTCCCCAGGTGTCAGTAGAGGTGGCCTTGCCCCCACCTGCAGAGCATGAAGTA
  AAGAAAGTGACTTTAGGAGATACCTTAACTCGACGTTCCATTAGCCAGCAGAAGTCCGGAGTTTCCATTA
  CCATTGATGACCCAGTCCGAACTGCCCAGGTGCCCTCCCCACCCCGGGGCAAGATTAGCAACATTGTCCA
  TATCTCCAATTTGGTCCGTCCTTTCACTTTAGGCCAGCTAAAGGAGTTGTTGGGGCGCACAGGAACCTTG
  GTGGAAGAGGCCTTCTGGATTGACAAGATCAAATCTCATTGCTTTGTAACGTACTCAACAGTAGAGGAAG
  CTGTTGCCACCCGCACAGCTCTGCACGGGGTCAAATGGCCCCAGTCCAATCCCAAATTCCTTTGTGCTGA
  CTATGCCGAGCAAGATGAGCTGGATTATCACCGAGGCCTCTTGGTGGACCGTCCCTCTGAAACTAAGACA
  GAGGAGCAGGGAATACCACGGCCCCTGCACCCCCCACCCCCACCCCCGGTCCAGCCACCACAGCACCCCC
  GGGCAGAGCAGCGGGAGCAGGAACGGGCAGTGCGGGAACAGTGGGCAGAACGGGAACGGGAAATGGAGCG
  GCGGGAGCGGACTCGATCAGAGCGTGAATGGGATCGGGACAAAGTTCGAGAAGGGCCCCGTTCCCGATCA
  AGGTCCCGTGACCGCCGCCGCAAGGAACGTGCGAAGTCTAAAGAAAAGAAGAGTGAGAAGAAAGAGAAAG
  CCCAGGAGGAACCACCTGCCAAGCTGCTGGATGACCTTTTCCGAAAGACCAAGGCAGCTCCCTGCATCTA
  TTGGCTCCCACTGACTGACAGCCAGATCGTTCAGAAAGAGGCAGAGCGGGCCGAACGGGCCAAGGAGCGG
  GAGAAGCGGCGAAAGGAGCAAGAAGAAGAAGAGCAAAAGGAGCGGGAGAAGGAAGCCGAGCGGGAACGGA
  ACCGACAGCTGGAGCGAGAGAAACGTCGGGAGCACAGTCGGGAGAGGGACAGGGAGAGAGAGAGAGAAAG
  GGAGCGGGACAGGGGGGACCGAGATCGGGATAGGGAAAGGGACCGAGAACGAGGCAGGGAAAGGGATCGC
  AGGGACACCAAGCGCCACAGCAGAAGCCGGAGTCGGAGCACACCTGTGCGGGACCGGGGTGGGCGCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228475 representing NM_001164817
  Red=Cloning site Green=Tags(s)
  
  MLSESKEGEEKEEVTMDTSENRPENDVPEPPMPIADQVSNDDRPEGSVEDEEKKESSLPKSFKRKISVVS
  ATKGVPAGNSDTEGGQPGRKRRWGASTATTQKKPSISITTESLKSLIPDIKPLAGQEAVVDLHADDSRIS
  EDETERNGDDGTHDKGLKICRTVTQVVPAEGQENGQREEEEEEKEPEAEPPVPPQVSVEVALPPPAEHEV
  KKVTLGDTLTRRSISQQKSGVSITIDDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTL
  VEEAFWIDKIKSHCFVTYSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKT
  EEQGIPRPLHPPPPPPVQPPQHPRAEQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRS
  RSRDRRRKERAKSKEKKSEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLTDSQIVQKEAERAERAKER
  EKRRKEQEEEEQKEREKEAERERNRQLEREKRREHSRERDRERERERERDRGDRDRDRERDRERGRERDR
  RDTKRHSRSRSRSTPVRDRGGRR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164817
• ORF Size: 1749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164817.1, NP_001158289.1
• RefSeq Size: 2529
• RefSeq ORF: 1752
• Locus ID: 22985
• Protein Pathways: Spliceosome
• Gene Summary: Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. This gene encodes a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. It may thus be involved in mRNA metabolism associated with splicing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]