PYGL (NM_001163940) Human Tagged ORF Clone

CAT#: RG228588

PYGL (GFP-tagged) - Human phosphorylase, glycogen, liver (PYGL), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PYGL
• Synonyms: GSD6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228588 representing NM_001163940
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGCCCCTGACGGACCAGGAGAAGCGGCGGCAGATCAGCATCCGCGGCATCGTGGGCGTGGAGA
  ACGTGGCAGAGCTGAAGAAGAGTTTCAACCGGCACCTGCACTTCACGCTGGTCAAGGACCGCAACGTGGC
  CACCACCCGCGACTACTACTTCGCGCTGGCGCACACGGTGCGCGACCACCTGGTGGGGCGCTGGATCCGC
  ACGCAGCAGCACTACTACGACAAGTGCCCCAAGCTTGGATTGGATATAGAAGAGTTAGAAGAAATTGAAG
  AAGATGCTGGACTTGGCAATGGTGGTCTTGGGAGACTTGCTGCCTGCTTCTTGGATTCCATGGCAACCCT
  GGGACTTGCAGCCTATGGATACGGCATTCGGTATGAATATGGGATTTTCAATCAGAAGATCCGAGATGGA
  TGGCAGGTAGAAGAAGCAGATGATTGGCTCAGATATGGAAACCCTTGGGAGAAGTCCCGCCCAGAATTCA
  TGCTGCCTGTGCACTTCTATGGAAAAGTAGAACACACCAACACCGGGACCAAGTGGATTGACACTCAAGT
  GGTCCTGGCTCTGCCATATGACACCCCCGTGCCCGGCTACATGAATAACACTGTCAACACCATGCGCCTC
  TGGTCTGCTCGGGCACCAAATGACTTTAACCTCAGAGACTTTAATGTTGGAGACTACATTCAGGCTGTGC
  TGGACCGAAACCTGGCCGAGAACATCTCCCGGGTCCTCTATCCCAATGACAATTTTTTTGAAGGGAAGGA
  GCTAAGATTGAAGCAGGAATACTTTGTGGTGGCTGCAACCTTGCAAGATATCATCCGCCGTTTCAAAGCC
  TCCAAGTTTGGCTCCACCCGTGGTGCAGGAACTGTGTTTGATGCCTTCCCGGATCAGGTGGCCATCCAGC
  TGAATGACACTCACCCTGCACTCGCGATCCCTGAGCTGATGAGGATTTTTGTGGATATTGAAAAACTGCC
  CTGGTCCAAGGCATGGGAGCTCACCCAGAAGACCTTCGCCTACACCAACCACACAGTGCTCCCGGAAGCC
  CTGGAGCGCTGGCCCGTGGACCTGGTGGAGAAGCTGCTCCCTCGACATTTGGAAATCATTTATGAGATAA
  ATCAGAAGCATTTAGATAGAATTGTGGCCTTGTTTCCTAAAGATGTGGACCGTCTGAGAAGGATGTCTCT
  GATAGAAGAGGAAGGAAGCAAAAGGATCAACATGGCCCATCTCTGCATTGTCGGTTCCCATGCTGTGAAT
  GGCGTGGCTAAAATCCACTCAGACATCGTGAAGACTAAAGTATTCAAGGACTTCAGTGAGCTAGAACCTG
  ACAAGTTTCAGAATAAAACCAATGGGATCACTCCAAGGCGCTGGCTCCTACTCTGCAACCCAGGACTTGC
  AGAGCTCATAGCAGAGAAAATTGGAGAAGACTATGTGAAAGACCTGAGCCAGCTGACGAAGCTCCACAGC
  TTCCTGGGTGATGATGTCTTCCTCCGGGAACTCGCCAAGGTGAAGCAGGAGAATAAGCTGAAGTTTTCTC
  AGTTCCTGGAGACGGAGTACAAAGTGAAGATCAACCCATCCTCCATGTTTGATGTCCAGGTGAAGAGGAT
  ACATGAGTACAAGCGACAGCTCTTGAACTGTCTGCATGTGATCACGATGTACAACCGCATTAAGAAAGAC
  CCTAAGAAGTTATTCGTGCCAAGGACAGTTATCATTGGTGGTAAAGCTGCCCCAGGATATCACATGGCCA
  AAATGATCATAAAGCTGATCACTTCAGTGGCAGATGTGGTGAACAATGACCCTATGGTTGGAAGCAAGTT
  GAAAGTCATCTTCTTGGAGAACTACAGAGTATCTCTTGCTGAAAAAGTCATTCCAGCCACAGATCTGTCA
  GAGCAGATTTCCACTGCAGGCACCGAAGCCTCGGGGACAGGCAATATGAAGTTCATGCTAAATGGGGCCC
  TAACTATCGGGACCATGGATGGGGCCAATGTGGAAATGGCAGAAGAAGCTGGGGAAGAGAACCTGTTCAT
  CTTTGGCATGAGGATAGATGATGTGGCTGCTTTGGACAAGAAAGGGTACGAGGCAAAAGAATACTATGAG
  GCACTTCCAGAGCTGAAGCTGGTCATTGATCAAATTGACAATGGCTTTTTTTCTCCCAAGCAGCCTGACC
  TCTTCAAAGATATCATCAACATGCTATTTTATCATGACAGGTTTAAAGTCTTTGCAGACTACGAAGCCTA
  TGTCAAGTGTCAAGATAAAGTGAGTCAGCTGTACATGAATCCAAAGGCCTGGAACACAATGGTACTCAAA
  AACATAGCTGCCTCGGGGAAATTCTCCAGTGACCGAACAATTAAAGAATATGCCCAAAACATCTGGAACG
  TGGAACCTTCAGATCTAAAGATTTCTCTATCCAATGAATCTAACAAAGTCAATGGAAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228588 representing NM_001163940
  Red=Cloning site Green=Tags(s)
  
  MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIR
  TQQHYYDKCPKLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDG
  WQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRL
  WSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKA
  SKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEA
  LERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVN
  GVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHS
  FLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKD
  PKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLS
  EQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYE
  ALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLK
  NIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163940
• ORF Size: 2439 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163940.1, NP_001157412.1
• RefSeq Size: 2757 bp
• RefSeq ORF: 2442 bp
• Locus ID: 5836
• Cytogenetics: 14q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism
• Gene Summary: 'This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011]'