DDHD1 (NM_001160147) Human Tagged ORF Clone

CAT#: RG228605

DDHD1 (GFP-tagged) - Human DDHD domain containing 1 (DDHD1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DDHD1
• Synonyms: PA-PLA1; PAPLA1; SPG28
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228605 representing NM_001160147
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTACCCGGGCCGCGGGTCCCCACGGAGCCCCGAGCATAACGGCCGAGGCGGCGGCGGCGGCGCCT
  GGGAGCTGGGCTCAGACGCGAGGCCAGCGTTCGGCGGCGGCGTCTGCTGCTTCGAGCACCTGCCCGGCGG
  GGACCCGGACGACGGCGACGTGCCCCTGGCCCTGCTGCGCGGGGAACCCGGGCTGCATTTGGCGCCGGGC
  ACCGACGACCACAACCACCACCTCGCGCTGGACCCCTGCCTCAGTGACGAGAACTATGACTTCAGCTCCG
  CCGAGTCGGGCTCCTCGCTGCGCTACTACAGCGAGGGTGAGAGCGGCGGCGGCGGCAGCTCCTTGTCGCT
  GCACCCGCCGCAGCAGCCTCCGCTGGTCCCGACGAACTCGGGGGGCGGCGGCGCGACAGGAGGGTCCCCC
  GGGGAAAGGAAACGTACCCGGCTTGGCGGCCCGGCGGCCCGGCACCGCTATGAGGTAGTGACGGAGCTGG
  GCCCGGAGGAGGTACGCTGGTTCTACAAGGAGGACAAGAAGACCTGGAAGCCCTTCATCGGCTACGACTC
  GCTCCGCATCGAGCTCGCCTTCCGGACCCTGCTGCAGACCACGGGTGCCCGGCCCCAGGGCGGGGACCGG
  GACGGCGACCATGTGTGCTCCCCCACGGGCCCAGCCTCCAGTTCCGGAGAAGATGACGATGAGGACCGCG
  CCTGCGGCTTCTGCCAGAGTACGACGGGGCACGAGCCGGAGATGGTGGAGCTTGTGAACATCGAGCCTGT
  GTGCGTGCGGGGCGGCCTCTACGAGGTGGATGTGACCCAAGGAGAGTGCTACCCGGTGTACTGGAACCAG
  GCTGATAAAATACCAGTAATGCGTGGACAGTGGTTTATTGACGGCACTTGGCAGCCTCTAGAAGAGGAAG
  AAAGTAATTTAATTGAGCAAGAACATCTCAATTGTTTTAGGGGCCAGCAGATGCAGGAAAATTTCGATAT
  TGAAGTGTCAAAATCCATAGATGGAAAAGATGGCAGTGGGATCAACTATTCTGCTGTTCATAGTTTCAAG
  TTGAGTCGAAACCATGTGGACTGGCACAGTGTGGATGAAGTATATCTTTATAGTGATGCAACAACATCTA
  AAATTGCAAGAACAGTTACCCAAAAACTGGGATTTTCTAAAGCATCAAGTAGTGGTACCAGACTTCATAG
  AGGTTATGTAGAAGAAGCCACATTAGAAGACAAGCCATCACAGACTACCCATATTGTATTTGTTGTGCAT
  GGCATTGGGCAGAAAATGGACCAAGGAAGAATTATCAAAAATACAGCTATGATGAGAGAAGCTGCAAGAA
  AAATAGAAGAAAGGCATTTTTCCAACCATGCAACACATGTTGAATTTCTGCCTGTTGAGTGGCGGTCAAA
  ACTTACTCTTGATGGAGACACTGTTGATTCCATTACTCCTGACAAAGTACGAGGTTTAAGGGATATGCTG
  AACAGCAGTGCAATGGACATAATGTATTATACTAGTCCACTTTATAGAGATGAACTAGTTAAAGGCCTTC
  AGCAAGAGCTGAATCGATTGTATTCCCTTTTCTGTTCTCGGAATCCAGACTTTGAAGAAAAAGGGGGTAA
  AGTCTCAATAGTATCACATTCCTTGGGATGTGTAATTACTTATGACATAATGACTGGCTGGAATCCAGTT
  CGGCTGTATGAACAGTTGCTGCAAAAGGAAGAAGAGTTGCCTGATGAACGATGGATGAGCTATGAAGAAC
  GACATCTTCTTGATGAACTCTATATAACTAAACGACGGCTGAAGGAAATAGAAGAACGGCTTCACGGATT
  GAAAGCATCATCTATGACACAAACACCTGCCTTAAAATTTAAGGTTGAGAATTTCTTCTGTATGGGATCC
  CCATTAGCAGTTTTCTTGGCGTTGCGTGGCATCCGCCCAGGAAATACTGGAAGTCAAGACCATATTTTGC
  CTAGAGAGATTTGTAACCGGTTACTAAATATTTTTCATCCTACAGATCCAGTGGCTTATAGATTAGAACC
  ATTAATACTGAAACACTACAGCAACATTTCACCTGTCCAGATCCACTGGTACAATACTTCAAATCCTTTA
  CCTTATGAACATATGAAGCCAAGCTTTCTCAACCCAGCTAAAGAACCTACCTCAGTTTCAGAGAATGAAG
  GCATTTCAACCATACCAAGCCCTGTGACCTCACCAGTTTTGTCCCGCCGACACTATGGAGAATCTATAAC
  AAATATAGGCAAAGCAAGCATATTAGGGGCTGCTAGCATTGGAAAGGGACTTGGAGGAATGTTGTTCTCA
  AGATTTGGACGTTCATCTACAACACAGTCATCTGAAACATCAAAAGACTCAATGGAAGATGAGAAGAAGC
  CAGTTGCCTCACCTTCTGCTACCACCGTAGGGACACAGACCCTTCCACATAGCAGTTCTGGCTTCCTCGA
  TTCTGCATTGGAGTTGGATCACAGGATTGATTTTGAACTCAGAGAAGGCCTTGTGGAGAGCCGCTATTGG
  TCAGCTGTCACGTCGCATACTGCCTATTGGTCATCCTTGGATGTTGCCCTTTTTCTTTTAACCTTCATGT
  ATAAACATGAGCACGATGATGATGCAAAACCCAATTTAGATCCAATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228605 representing NM_001160147
  Red=Cloning site Green=Tags(s)
  
  MNYPGRGSPRSPEHNGRGGGGGAWELGSDARPAFGGGVCCFEHLPGGDPDDGDVPLALLRGEPGLHLAPG
  TDDHNHHLALDPCLSDENYDFSSAESGSSLRYYSEGESGGGGSSLSLHPPQQPPLVPTNSGGGGATGGSP
  GERKRTRLGGPAARHRYEVVTELGPEEVRWFYKEDKKTWKPFIGYDSLRIELAFRTLLQTTGARPQGGDR
  DGDHVCSPTGPASSSGEDDDEDRACGFCQSTTGHEPEMVELVNIEPVCVRGGLYEVDVTQGECYPVYWNQ
  ADKIPVMRGQWFIDGTWQPLEEEESNLIEQEHLNCFRGQQMQENFDIEVSKSIDGKDGSGINYSAVHSFK
  LSRNHVDWHSVDEVYLYSDATTSKIARTVTQKLGFSKASSSGTRLHRGYVEEATLEDKPSQTTHIVFVVH
  GIGQKMDQGRIIKNTAMMREAARKIEERHFSNHATHVEFLPVEWRSKLTLDGDTVDSITPDKVRGLRDML
  NSSAMDIMYYTSPLYRDELVKGLQQELNRLYSLFCSRNPDFEEKGGKVSIVSHSLGCVITYDIMTGWNPV
  RLYEQLLQKEEELPDERWMSYEERHLLDELYITKRRLKEIEERLHGLKASSMTQTPALKFKVENFFCMGS
  PLAVFLALRGIRPGNTGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKHYSNISPVQIHWYNTSNPL
  PYEHMKPSFLNPAKEPTSVSENEGISTIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFS
  RFGRSSTTQSSETSKDSMEDEKKPVASPSATTVGTQTLPHSSSGFLDSALELDHRIDFELREGLVESRYW
  SAVTSHTAYWSSLDVALFLLTFMYKHEHDDDAKPNLDPI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160147
• ORF Size: 2637 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001160147.1, NP_001153619.1
• RefSeq Size: 12906
• RefSeq ORF: 2640
• Locus ID: 80821
• Gene Summary: This gene is a member of the intracellular phospholipase A1 gene family. The protein encoded by this gene preferentially hydrolyzes phosphatidic acid. It is a cytosolic protein with some mitochondrial localization, and is thought to be involved in the regulation of mitochondrial dynamics. Overexpression of this gene causes fragmentation of the tubular structures in mitochondria, while depletion of the gene results in mitochondrial tubule elongation. Deletion of this gene in male mice caused fertility defects, resulting from disruption in the organization of the mitochondria during spermiogenesis. In humans, mutations in this gene have been associated with hereditary spastic paraplegia (HSP), also known as Strumpell-Lorrain disease, or, familial spastic paraparesis (FSP). This inherited disorder is characterized by progressive weakness and spasticity of the legs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]