Filamin B (FLNB) (NM_001164319) Human Tagged ORF Clone
CAT#: RG228710
- TrueORF®
FLNB (GFP-tagged) - Human filamin B, beta (FLNB), transcript variant 4
"NM_001164319" in other vectors (2)
Product Images
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Other products for "FLNB"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | FLNB |
Synonyms | ABP-278; ABP-280; AOI; FH1; FLN-B; FLN1L; LRS1; SCT; TABP; TAP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228710 representing NM_001164319
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTAACCGAGAAGGATCTAGCTGAGGACGCGCCTTGGAAGAAGATCCAGCAGAACACGTTCACAC GCTGGTGCAACGAGCACCTCAAGTGCGTGAACAAACGCATCGGCAACCTGCAGACCGACCTGAGCGACGG GCTGCGGCTCATCGCGCTGCTCGAGGTGCTCAGCCAGAAGCGCATGTACCGCAAGTACCATCAGCGGCCC ACCTTTCGCCAGATGCAGCTCGAGAATGTGTCCGTGGCGCTCGAGTTCCTGGACCGTGAGAGCATCAAGC TCGTGTCCATCGATAGCAAAGCCATTGTGGATGGGAACCTGAAGCTCATCTTGGGTCTGGTGTGGACGCT GATCCTCCACTACTCCATCTCCATGCCCGTGTGGGAGGATGAAGGGGATGATGATGCCAAGAAGCAGACG CCAAAGCAGAGGCTGCTGGGGTGGATTCAGAACAAGATCCCCTACTTGCCCATCACCAACTTTAACCAGA ACTGGCAAGACGGCAAAGCCCTGGGAGCCCTGGTAGACAGCTGTGCTCCAGGTCTGTGCCCAGACTGGGA ATCCTGGGACCCGCAGAAGCCTGTGGATAATGCACGAGAAGCCATGCAGCAGGCAGATGACTGGCTGGGT GTCCCACAGGTCATCACTCCTGAAGAAATCATTCACCCGGATGTGGACGAGCACTCAGTTATGACTTACC TGTCCCAGTTCCCCAAAGCCAAGCTCAAGCCGGGGGCTCCTCTCAAACCCAAACTCAACCCGAAGAAAGC CAGGGCCTATGGCAGAGGAATCGAGCCCACTGGAAACATGGTGAAGCAGCCAGCCAAGTTCACTGTGGAC ACCATCAGCGCCGGGCAAGGAGACGTGATGGTGTTTGTTGAGGACCCAGAAGGGAACAAAGAGGAGGCAC AAGTGACCCCTGACAGTGACAAGAACAAGACATACTCTGTGGAGTATCTGCCCAAGGTCACCGGGCTACA CAAAGTCACAGTCCTCTTTGCAGGACAGCACATCTCCAAGAGCCCATTTGAAGTGAGTGTTGACAAGGCC CAGGGAGATGCCAGTAAAGTCACTGCAAAAGGTCCAGGGTTGGAAGCTGTAGGGAACATCGCCAATAAGC CCACCTACTTTGACATCTATACGGCAGGAGCTGGTGTGGGTGACATTGGTGTGGAGGTGGAAGATCCCCA GGGGAAGAACACCGTGGAGTTGCTCGTGGAAGACAAAGGAAACCAGGTGTATCGATGTGTGTACAAACCC ATGCAGCCTGGCCCTCACGTGGTCAAGATCTTCTTTGCTGGGGACACTATTCCTAAGAGTCCCTTCGTTG TGCAGGTTGGGGAAGCCTGCAATCCAAATGCCTGCCGGGCCAGTGGCCGAGGCCTACAACCCAAAGGCGT CCGTATCCGGGAGACCACAGATTTCAAGGTTGACACCAAAGCTGCAGGAAGTGGGGAGCTCGGTGTAACC ATGAAGGGTCCTAAGGGTCTGGAGGAGCTGGTGAAGCAGAAAGACTTTCTGGATGGGGTCTACGCATTCG AGTATTACCCCAGCACCCCGGGGAGATACAGCATTGCCATCACATGGGGGGGACACCACATTCCAAAGAG CCCCTTTGAAGTTCAAGTTGGCCCTGAAGCGGGTATGCAGAAAGTCCGTGCTTGGGGCCCTGGGCTCCAT GGTGGGATTGTCGGGCGGTCAGCGGACTTCGTGGTAGAATCCATTGGCTCTGAAGTGGGGTCTCTGGGGT TTGCCATTGAAGGCCCCTCTCAGGCAAAGATTGAGTACAACGACCAGAATGATGGATCGTGTGATGTCAA ATACTGGCCCAAGGAGCCTGGCGAATATGCTGTTCACATCATGTGTGACGACGAAGACATCAAGGACAGC CCGTACATGGCCTTCATCCACCCAGCCACGGGAGGCTACAACCCTGATCTGGTTCGAGCATACGGGCCAG GTTTGGAGAAATCTGGATGCATTGTCAACAACCTGGCCGAGTTCACTGTGGATCCTAAGGATGCTGGAAA AGCTCCCTTAAAGATATTTGCTCAGGATGGGGAAGGCCAACGCATTGACATCCAGATGAAGAACCGGATG GACGGCACATATGCATGCTCATACACCCCGGTGAAGGCCATCAAGCACACCATTGCTGTGGTCTGGGGAG GCGTGAACATCCCGCACAGCCCCTACAGGGTCAACATCGGGCAAGGTAGCCATCCTCAGAAGGTCAAAGT GTTTGGGCCAGGTGTGGAGAGAAGTGGTCTGAAGGCAAATGAACCTACACACTTCACGGTGGACTGTACT GAGGCTGGGGAAGGTGATGTCAGTGTTGGCATTAAGTGTGATGCCCGGGTGTTAAGTGAAGATGAGGAAG ACGTGGATTTTGACATTATTCACAATGCCAATGATACGTTCACAGTCAAATATGTGCCTCCTGCTGCTGG GCGATACACTATCAAAGTTCTCTTTGCATCTCAGGAAATCCCCGCCAGCCCTTTCAGAGTCAAAGTTGAC CCTTCCCACGATGCCAGCAAAGTGAAGGCAGAAGGCCCAGGGCTCAGCAAAGCAGGTGTGGAAAATGGGA AACCGACCCACTTCACTGTCTACACCAAGGGGGCTGGGAAAGCCCCGCTCAACGTGCAGTTCAACAGCCC TCTTCCTGGCGATGCAGTGAAGGATTTGGATATCATCGATAATTATGACTACTCTCACACGGTTAAATAT ACACCCACCCAACAGGGCAACATGCAGGTTCTGGTGACTTACGGTGGCGATCCCATCCCTAAAAGCCCTT TCACTGTGGGTGTTGCTGCACCGCTGGATCTGAGCAAGATAAAACTCAATGGGCTGGAAAACAGGGTGGA AGTTGGGAAGGATCAGGAGTTCACCGTTGATACCAGGGGGGCAGGAGGCCAGGGGAAGCTGGACGTGACA ATCCTCAGCCCCTCTCGGAAGGTCGTGCCATGCCTAGTGACACCTGTGACAGGCCGGGAGAACAGCACGG CCAAGTTCATCCCTCGGGAGGAGGGGCTGTATGCTGTAGACGTGACCTACGATGGACACCCTGTGCCCGG GAGCCCCTACACAGTGGAGGCCTCGCTGCCACCAGATCCCAGCAAGGTGAAGGCCCACGGTCCCGGCCTC GAAGGTGGTCTCGTGGGCAAGCCTGCCGAGTTCACCATCGATACCAAAGGAGCTGGTACTGGAGGTCTGG GCTTAACGGTGGAAGGTCCGTGCGAGGCCAAAATCGAGTGCTCCGACAATGGTGATGGGACCTGCTCCGT CTCTTACCTTCCCACAAAACCCGGGGAGTACTTCGTCAACATCCTCTTTGAAGAAGTCCACATACCTGGG TCTCCCTTCAAAGCTGACATTGAAATGCCCTTTGACCCCTCTAAAGTCGTGGCATCGGGGCCAGGTCTCG AGCACGGGAAGGTGGGTGAAGCTGGCCTCCTTAGCGTCGACTGCTCGGAAGCGGGACCGGGGGCCCTGGG CCTGGAAGCTGTCTCGGACTCGGGAACAAAAGCCGAAGTCAGTATTCAGAACAACAAAGATGGCACCTAC GCGGTGACCTACGTGCCCCTGACGGCCGGCATGTACACGTTGACCATGAAGTATGGTGGCGAACTCGTGC CACACTTCCCCGCCCGGGTCAAGGTGGAGCCCGCCGTGGACACCAGCAGGATCAAAGTCTTTGGACCAGG AATAGAAGGGAAAGATGTGTTCCGGGAAGCTACCACCGACTTTACAGTTGACTCTCGGCCGCTGACCCAG GTTGGGGGTGACCACATCAAGGCCCACATTGCCAACCCCTCAGGGGCCTCCACCGAGTGCTTTGTCACAG ACAATGCGGATGGGACCTACCAGGTGGAATACACACCCTTTGAGAAAGGTCTCCATGTAGTGGAGGTGAC ATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCACTGAAGGCTGCCAGCCATCTAGG GTGCAAGCCCAAGGACCTGGATTGAAAGAGGCCTTTACCAACAAGCCCAATGTCTTCACCGTGGTTACCA GAGGCGCAGGAATTGGTGGGCTTGGCATAACTGTTGAGGGACCATCAGAGTCGAAGATAAATTGCAGAGA CAACAAGGATGGCAGCTGCAGTGCTGAGTACATTCCTTTCGCACCGGGGGATTACGATGTTAATATCACA TATGGAGGAGCCCACATCCCCGGCAGCCCCTTCAGGGTTCCTGTGAAGGATGTTGTGGACCCCAGCAAGG TCAAGATTGCCGGCCCCGGGCTGGGCTCAGGCGTCCGAGCCCGTGTCCTGCAGTCCTTCACGGTGGACAG CAGCAAGGCTGGCCTGGCTCCGCTGGAAGTGAGGGTTCTGGGCCCACGAGGCTTGGTGGAGCCAGTGAAC GTGGTGGACAATGGAGATGGCACACACACAGTAACCTACACCCCATCTCAGGAGGGACCTTACATGGTCT CAGTTAAATATGCTGATGAAGAGATTCCTCGCAGTCCCTTCAAGGTCAAGGTCCTTCCCACATATGATGC CAGCAAAGTGACTGCCAGTGGCCCCGGCCTTAGTTCCTATGGTGTGCCTGCCAGTCTACCTGTGGACTTT GCAATTGATGCCCGAGATGCCGGGGAAGGCCTGCTTGCTGTTCAAATAACGGACCAAGAAGGAAAACCCA AAAGAGCCATTGTCCATGACAATAAAGATGGCACGTATGCTGTCACCTACATCCCCGACAAGACTGGGCG CTATATGATTGGAGTCACCTACGGGGGTGACGACATCCCACTTTCTCCTTATCGCATCCGAGCCACACAG ACGGGTGATGCCAGCAAGTGCCTGGCCACGGGTCCTGGAATCGCCTCCACTGTGAAAACTGGCGAAGAAG TAGGCTTTGTGGTTGATGCCAAGACTGCCGGGAAGGGTAAAGTGACCTGCACGGTTCTGACCCCAGATGG CACTGAGGCCGAGGCCGATGTCATTGAGAATGAAGATGGAACCTATGACATCTTCTACACAGCTGCCAAG CCGGGCACATATGTGATCTATGTGCGCTTCGGTGGTGTTGATATTCCTAACAGCCCCTTCACTGTCATGG TGACCGAAGAGGCCTATGTCCCAGTGAGTGACATGAACGGCCTGGGATTTAAGCCTTTTGACCTGGTCAT TCCGTTTGCTGTCAGGAAAGGAGAAATCACTGGAGAGGTCCACATGCCTTCTGGGAAGACAGCCACACCT GAGATTGTGGACAACAAGGACGGCACGGTCACTGTTAGATATGCCCCCACTGAGGTCGGGCTCCATGAGA TGCACATCAAATACATGGGCAGCCACATCCCTGAGAGCCCACTCCAGTTCTACGTGAACTACCCCAACAG TGGAAGTGTTTCTGCATACGGTCCAGGCCTCGTGTATGGAGTGGCCAACAAAACTGCCACCTTCACCATC GTCACAGAGGATGCAGGAGAAGGTGGTCTGGACTTGGCTATTGAGGGCCCCTCAAAAGCAGAAATCAGCT GCATTGACAATAAAGATGGGACATGCACAGTGACCTACCTGCCGACTCTGCCAGGCGACTACAGCATTCT GGTCAAGTACAATGACAAGCACATCCCTGGCAGCCCCTTCACAGCCAAGATCACAGATGACAGCAGGCGG TGCTCCCAGGTGAAGTTGGGCTCAGCCGCTGACTTCCTGCTCGACATCAGTGAGACTGACCTCAGCAGCC TGACGGCCAGCATTAAGGCCCCATCTGGCCGAGACGAGCCCTGTCTCCTGAAGAGGCTGCCCAACAACCA CATTGGCATCTCCTTCATCCCCCGGGAAGTGGGCGAACATCTGGTCAGCATCAAGAAAAATGGCAACCAT GTGGCCAACAGCCCCGTGTCTATCATGGTGGTCCAGTCGGAGATTGGTGACGCCCGCCGAGCCAAAGTCT ATGGCCGCGGCCTGTCAGAAGGCCGGACTTTCGAGATGTCTGACTTCATCGTGGACACAAGGGATGCAGG TTATGGTGGCATATCCTTGGCGGTGGAAGGCCCCAGCAAAGTGGACATCCAGACGGAGGACCTGGAAGAT GGCACCTGCAAAGTCTCCTACTTCCCTACCGTGCCTGGGGTTTATATCGTCTCCACCAAATTCGCTGACG AGCACGTGCCTGGGAGCCCATTTACCGTGAAGATCAGTGGGGAGGGAAGAGTCAAAGAGAGCATCACCCG CACCAGTCGGGCCCCGTCCGTGGCCACTGTCGGGAGCATTTGTGACCTGAACCTGAAAATCCCAGAAATC AACAGCAGTGATATGTCGGCCCACGTCACCAGCCCCTCTGGCCGTGTGACTGAGGCAGAGATTGTGCCCA TGGGGAAGAACTCACACTGCGTCCGGTTTGTGCCCCAGGAGATGGGCGTGCACACGGTCAGCGTCAAGTA CCGTGGGCAGCACGTCACCGGCAGCCCCTTCCAGTTCACCGTGGGGCCACTTGGTGAAGGAGGCGCCCAC AAGGTGCGGGCAGGAGGCCCTGGCCTGGAGAGAGGAGAAGCGGGAGTCCCAGCTGAGTTCAGCATTTGGA CCCGGGAAGCAGGCGCTGGAGGCCTCTCCATCGCTGTTGAGGGCCCCAGTAAGGCCGAGATTACATTCGA TGACCATAAAAATGGGTCGTGCGGTGTATCTTATATTGCCCAAGAGCCTGGTAACTACGAGGTGTCCATC AAGTTCAATGATGAGCACATCCCGGAAAGCCCCTACCTGGTGCCGGTCATCGCACCCTCCGACGACGCCC GCCGCCTCACTGTTATGAGCCTTCAGGAATCGGGATTAAAAGTTAACCAGCCAGCATCCTTTGCTATAAG GTTGAATGGCGCAAAAGGCAAGATTGATGCAAAGGTGCACAGCCCCTCTGGAGCCGTGGAGGAGTGCCAC GTGTCTGAGCTGGAGCCAGATAAGTATGCTGTTCGCTTCATCCCTCATGAGAATGGTGTCCACACCATCG ATGTCAAGTTCAATGGGAGCCACGTGGTTGGAAGCCCCTTCAAAGTGCGCGTTGGGGAGCCTGGACAAGC GGGGAACCCTGCCCTGGTGTCCGCCTATGGCACGGGACTCGAAGGGGGCACCACAGGTATCCAGTCGGAA TTCTTTATTAACACCACCCGAGCAGGTCCAGGGACATTATCCGTCACCATCGAAGGCCCATCCAAGGTTA AAATGGATTGCCAGGAAACACCTGAAGGGTACAAAGTCATGTACACCCCCATGGCTCCTGGTAACTACCT GATCAGCGTCAAATACGGTGGGCCCAACCACATCGTGGGCAGTCCCTTCAAGGCCAAGGTGACAGGCCAG CGTCTAGTTAGCCCTGGCTCAGCCAACGAGACCTCATCCATCCTGGTGGAGTCAGTGACCAGGTCGTCTA CAGAGACCTGCTATAGCGCCATTCCCAAGGCATCCTCGGACGCCAGCAAGGTGACCTCTAAGGGGGCAGG GCTCTCAAAGGCCTTTGTGGGCCAGAAGAGTTCCTTCCTGGTGGACTGCAGCAAAGCTGGCTCCAACATG CTGCTGATCGGGGTCCATGGGCCCACCACCCCCTGCGAGGAGGTCTCCATGAAGCATGTAGGCAACCAGC AATACAACGTCACATACGTCGTCAAGGAGAGGGGCGATTATGTGCTGGCTGTGAAGTGGGGGGAGGAACA CATCCCTGGCAGCCCTTTTCATGTCACAGTGCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228710 representing NM_001164319
Red=Cloning site Green=Tags(s) MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKRMYRKYHQRP TFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQT PKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPQKPVDNAREAMQQADDWLG VPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVD TISAGQGDVMVFVEDPEGNKEEAQVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVSVDKA QGDASKVTAKGPGLEAVGNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDKGNQVYRCVYKP MQPGPHVVKIFFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGVRIRETTDFKVDTKAAGSGELGVT MKGPKGLEELVKQKDFLDGVYAFEYYPSTPGRYSIAITWGGHHIPKSPFEVQVGPEAGMQKVRAWGPGLH GGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYNDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDS PYMAFIHPATGGYNPDLVRAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGEGQRIDIQMKNRM DGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVNIGQGSHPQKVKVFGPGVERSGLKANEPTHFTVDCT EAGEGDVSVGIKCDARVLSEDEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPASPFRVKVD PSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFNSPLPGDAVKDLDIIDNYDYSHTVKY TPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSKIKLNGLENRVEVGKDQEFTVDTRGAGGQGKLDVT ILSPSRKVVPCLVTPVTGRENSTAKFIPREEGLYAVDVTYDGHPVPGSPYTVEASLPPDPSKVKAHGPGL EGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPG SPFKADIEMPFDPSKVVASGPGLEHGKVGEAGLLSVDCSEAGPGALGLEAVSDSGTKAEVSIQNNKDGTY AVTYVPLTAGMYTLTMKYGGELVPHFPARVKVEPAVDTSRIKVFGPGIEGKDVFREATTDFTVDSRPLTQ VGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPIPNSPFKVAVTEGCQPSR VQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYIPFAPGDYDVNIT YGGAHIPGSPFRVPVKDVVDPSKVKIAGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVLGPRGLVEPVN VVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSSYGVPASLPVDF AIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQ TGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAK PGTYVIYVRFGGVDIPNSPFTVMVTEEAYVPVSDMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTATP EIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTI VTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRR CSQVKLGSAADFLLDISETDLSSLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNH VANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLED GTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEI NSSDMSAHVTSPSGRVTEAEIVPMGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAH KVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSI KFNDEHIPESPYLVPVIAPSDDARRLTVMSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECH VSELEPDKYAVRFIPHENGVHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGTGLEGGTTGIQSE FFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLISVKYGGPNHIVGSPFKAKVTGQ RLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFVGQKSSFLVDCSKAGSNM LLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001164319 |
ORF Size | 7734 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164319.1, NP_001157791.1 |
RefSeq Size | 9395 bp |
RefSeq ORF | 7737 bp |
Locus ID | 2317 |
Cytogenetics | 3p14.3 |
Protein Pathways | Focal adhesion, MAPK signaling pathway |
Gene Summary | 'This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]' |
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