Dysadherin (FXYD5) (NM_001164605) Human Tagged ORF Clone

CAT#: RG228748

FXYD5 (GFP-tagged) - Human FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FXYD5
• Synonyms: DYSAD; HSPC113; IWU1; KCT1; OIT2; PRO6241; RIC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228748 representing NM_001164605
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCCTCTGGTCGCCTGTGTCTTCTTACCATCGTTGGCCTGATTCTCCCCACCAGAGGACAGACGT
  TGAAAGATACCACGTCCAGTTCTTCAGCAGACTCAACTATCATGGACATTCAGGTCCCGACACGAGCCCC
  AGATGCAGTCTACACAGAACTCCAGCCCACCTCTCCAACCCCAACCTGGCCTGCTGATGAAACACCACAA
  CCCCAGACCCAGACCCAGCAACTGGAAGGAACGGATGGGCCTCTAGTGACAGATCCAGAGACACACAAGA
  GCACCAAAGCAGCTCATCCCACTGATGACACCACGACGCTCTCTGAGAGACCATCCCCAAGCACAGACGT
  CCAGACAGACCCCCAGACCCTCAAGCCATCTGGTTTTCATGAGGATGACCCCTTCTTCTATGATGAACAC
  ACCCTCCGGAAACGGGGGCTGTTGGTCGCAGCTGTGCTGTTCATCACAGGCATCATCATCCTCACCAGTG
  GCAAGTGCAGGCAGCTGTCCCGGTTATGCCGGAATCATTGCAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228748 representing NM_001164605
  Red=Cloning site Green=Tags(s)
  
  MSPSGRLCLLTIVGLILPTRGQTLKDTTSSSSADSTIMDIQVPTRAPDAVYTELQPTSPTPTWPADETPQ
  PQTQTQQLEGTDGPLVTDPETHKSTKAAHPTDDTTTLSERPSPSTDVQTDPQTLKPSGFHEDDPFFYDEH
  TLRKRGLLVAAVLFITGIIILTSGKCRQLSRLCRNHCR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164605
• ORF Size: 534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164605.1, NP_001158077.1
• RefSeq Size: 917
• RefSeq ORF: 537
• Locus ID: 53827
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, is a glycoprotein that functions in the up-regulation of chemokine production, and it is involved in the reduction of cell adhesion via its ability to down-regulate E-cadherin. It also promotes metastasis, and has been linked to a variety of cancers. Alternative splicing results in multiple transcript variants. [RefSeq curation by Kathleen J. Sweadner, Ph.D., sweadner@helix.mgh.harvard.edu., Sep 2009]