PDP1 (NM_001161781) Human Tagged ORF Clone

CAT#: RG228947

PDP1 (GFP-tagged) - Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDP1
• Synonyms: PDH; PDP; PDPC; PPM2A; PPM2C
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228947 representing NM_001161781
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCACCAACTCAACTGTTTTTTCCTCTCATCCGTAACTGTGAACTGAGCAGGATCTATGGCACTG
  CATGTTACTGCCACCACAAACATCTCTGTTGTTCCTCATCGTACATTCCTCAGAGTCGACTGAGATACAC
  ACCTCATCCAGCATATGCTACCTTTTGCAGGCCAAAGGAGAACTGGTGGCAGTACACCCAAGGAAGGAGA
  TATGCTTCCACACCACAGAAATTTTACCTCACACCTCCACAAGTCAATAGCATCCTTAAAGCTAATGAAT
  ACAGTTTCAAAGTGCCAGAATTTGACGGCAAAAATGTCAGTTCTATCCTTGGATTTGACAGCAATCAGCT
  GCCTGCAAATGCACCCATTGAGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGCTTTTG
  GGGGTTTTTGATGGCCATGCAGGTTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTTTATTATATTG
  CTGTCTCTTTGTTACCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTGGAGAGCGGCCGGGCACTGCT
  ACCCATTCTCCAGTGGCACAAGCACCCCAATGATTACTTTAGTAAGGAGGCATCCAAATTGTACTTTAAC
  AGCTTGAGGACTTACTGGCAAGAGCTTATAGACCTCAACACTGGTGAGTCGACTGATATTGATGTTAAGG
  AGGCTCTAATTAATGCCTTCAAGAGGCTTGATAATGACATCTCCTTGGAGGCGCAAGTTGGTGATCCTAA
  TTCTTTTCTCAACTACCTGGTGCTTCGAGTGGCATTTTCTGGAGCCACTGCTTGTGTGGCCCATGTGGAT
  GGTGTTGACCTTCATGTGGCCAATACTGGCGATAGCAGAGCCATGCTGGGTGTGCAGGAAGAGGACGGCT
  CATGGTCAGCAGTCACGCTGTCTAATGACCACAATGCTCAAAATGAAAGAGAACTAGAACGGCTGAAATT
  GGAACATCCAAAGAGTGAGGCCAAGAGTGTCGTGAAACAGGATCGGCTGCTTGGCTTGCTGATGCCATTT
  AGGGCATTTGGAGATGTAAAGTTCAAATGGAGCATTGACCTTCAAAAGAGAGTGATAGAATCTGGCCCAG
  ACCAGTTGAATGACAATGAATATACCAAGTTTATTCCTCCTAATTATCACACACCTCCTTATCTCACTGC
  TGAGCCAGAGGTAACTTACCACCGATTAAGGCCACAGGATAAGTTTCTGGTGTTGGCTACTGATGGGTTG
  TGGGAGACTATGCATAGGCAGGATGTGGTTAGGATTGTGGGTGAGTACCTAACTGGCATGCATCACCAAC
  AGCCAATAGCTGTTGGTGGCTACAAGGTGACTCTGGGACAGATGCATGGCCTTTTAACAGAAAGGAGAAC
  CAAAATGTCCTCGGTATTTGAGGATCAGAACGCAGCAACCCATCTCATTCGCCACGCTGTGGGCAACAAC
  GAGTTTGGGACTGTTGATCATGAGCGCCTCTCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCGAATGT
  ACAGAGATGACATTACAATCATTGTAGTTCAGTTCAATTCTCATGTTGTAGGGGCGTATCAAAACCAAGA
  A
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228947 representing NM_001161781
  Red=Cloning site Green=Tags(s)
  
  MPAPTQLFFPLIRNCELSRIYGTACYCHHKHLCCSSSYIPQSRLRYTPHPAYATFCRPKENWWQYTQGRR
  YASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLL
  GVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFN
  SLRTYWQELIDLNTGESTDIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACVAHVD
  GVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKSEAKSVVKQDRLLGLLMPF
  RAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGL
  WETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRTKMSSVFEDQNAATHLIRHAVGNN
  EFGTVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161781
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161781.1, NP_001155253.1
• RefSeq Size: 4234
• RefSeq ORF: 1614
• Locus ID: 54704
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2009]