SLI (SHC2) (NM_012435) Human Tagged ORF Clone

CAT#: RG229293

SHC2 (GFP-tagged) - Human SHC (Src homology 2 domain containing) transforming protein 2 (SHC2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SHC2
• Synonyms: SCK; SHCB; SLI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229293 representing NM_012435
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCAGGGTCCGGGCGGGCGCGCGCCCCCGGCGCCCCCCGCGCCCCCCGAGCCCGAGGCGCCCACCA
  CCTTCTGCGCGTTGCTGCCCCGAATGCCGCAGTGGAAGTTCGCGGCCCCGGGCGGCTTCCTGGGCCGCGG
  CCCGGCGGCGGCGCGGGCGGCTGGGGCCTCGGGGGGCGCGGACCCCCAACCCGAGCCGGCGGGCCCGGGG
  GGCGTCCCGGCGCTGGCGGCCGCCGTCCTGGGCGCCTGCGAGCCCCGCTGCGCCGCGCCCTGTCCCCTGC
  CCGCGCTCAGCCGCTGTCGGGGCGCGGGGTCGCGGGGGTCGCGGGGCGGGCGGGGGGCGGCGGGGTCCGG
  GGACGCCGCCGCCGCCGCCGAGTGGATCCGGAAGGGCAGCTTCATCCACAAACCCGCGCACGGCTGGCTA
  CACCCCGACGCCAGGGTCCTGGGGCCCGGGGTCTCCTACGTCGTGCGGTACATGGGCTGCATCGAGGTTC
  TCCGCTCTATGCGCTCCCTGGACTTTAACACGCGCACGCAGGTGACCAGGGAAGCCATCAACCGGCTCCA
  TGAGGCCGTGCCTGGCGTCCGGGGATCCTGGAAGAAAAAGGCCCCCAACAAGGCCCTGGCGTCCGTCCTG
  GGCAAGAGCAACCTTCGCTTTGCCGGCATGAGCATCTCCATCCACATCTCCACTGATGGCCTCAGCCTCT
  CCGTGCCTGCCACGCGCCAGGTCATCGCCAACCACCACATGCCGTCCATCTCCTTCGCGTCAGGCGGAGA
  CACGGACATGACGGATTACGTGGCCTACGTCGCCAAGGACCCCATCAACCAGAGAGCCTGCCACATCCTG
  GAGTGCTGTGAGGGCCTGGCACAGAGCATCATCAGCACCGTGGGCCAAGCTTTCGAGCTGCGCTTCAAGC
  AGTACCTGCACAGCCCGCCCAAGGTGGCGCTGCCCCCAGAAAGGCTGGCAGGGCCGGAGGAGTCGGCCTG
  GGGGGACGAGGAGGACTCTTTGGAGCACAATTACTACAACAGCATCCCGGGGAAGGAGCCGCCGCTGGGC
  GGGCTAGTGGACTCCAGGCTGGCCCTGACACAGCCCTGCGCCCTCACGGCCCTCGACCAGGGCCCATCTC
  CTTCTCTAAGAGATGCCTGCAGCCTGCCATGGGACGTGGGGTCCACCGGTACAGCTCCACCGGGGGACGG
  CTACGTGCAGGCGGACGCCCGGGGCCCCCCGGACCACGAGGAGCACCTGTATGTCAACACCCAGGGTCTG
  GACGCCCCCGAGCCGGAGGACAGCCCCAAAAAGGATCTGTTTGACATGCGACCCTTTGAGGATGCCCTGA
  AGTTGCATGAGTGCTCAGTGGCGGCAGGCGTGACAGCAGCCCCTCTTCCCTTGGAGGACCAGTGGCCCAG
  CCCCCCTACCCGCCGGGCCCCTGTGGCCCCCACGGAGGAACAGCTGCGTCAGGAGCCCTGGTACCACGGC
  CGGATGAGCCGCCGGGCGGCAGAGAGGATGCTTCGAGCTGACGGGGACTTCCTTGTGCGAGACAGCGTCA
  CCAACCCCGGGCAGTATGTCCTCACCGGCATGCACGCCGGGCAGCCCAAGCACCTGCTGCTCGTGGACCC
  CGAGGGCGTGGTACGGACGAAGGACGTGCTGTTTGAGAGCATCAGCCACCTGATCGACCACCACCTGCAG
  AACGGGCAGCCCATCGTGGCCGCCGAGAGTGAGCTGCACCTGCGTGGCGTGGTCTCACGGGAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229293 representing NM_012435
  Red=Cloning site Green=Tags(s)
  
  MTQGPGGRAPPAPPAPPEPEAPTTFCALLPRMPQWKFAAPGGFLGRGPAAARAAGASGGADPQPEPAGPG
  GVPALAAAVLGACEPRCAAPCPLPALSRCRGAGSRGSRGGRGAAGSGDAAAAAEWIRKGSFIHKPAHGWL
  HPDARVLGPGVSYVVRYMGCIEVLRSMRSLDFNTRTQVTREAINRLHEAVPGVRGSWKKKAPNKALASVL
  GKSNLRFAGMSISIHISTDGLSLSVPATRQVIANHHMPSISFASGGDTDMTDYVAYVAKDPINQRACHIL
  ECCEGLAQSIISTVGQAFELRFKQYLHSPPKVALPPERLAGPEESAWGDEEDSLEHNYYNSIPGKEPPLG
  GLVDSRLALTQPCALTALDQGPSPSLRDACSLPWDVGSTGTAPPGDGYVQADARGPPDHEEHLYVNTQGL
  DAPEPEDSPKKDLFDMRPFEDALKLHECSVAAGVTAAPLPLEDQWPSPPTRRAPVAPTEEQLRQEPWYHG
  RMSRRAAERMLRADGDFLVRDSVTNPGQYVLTGMHAGQPKHLLLVDPEGVVRTKDVLFESISHLIDHHLQ
  NGQPIVAAESELHLRGVVSREP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012435
• ORF Size: 1746 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012435.2, NP_036567.2
• RefSeq Size: 2509
• RefSeq ORF: 1749
• Locus ID: 25759
• Protein Pathways: Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, VEGF signaling pathway