SHANK2 (NM_012309) Human Tagged ORF Clone

CAT#: RG229453

SHANK2 (GFP-tagged) - Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SHANK2
• Synonyms: AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK; SPANK-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229453 representing NM_012309
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCGCAGCCCAACATCCAGCGAGGACGAGATGGCCCAGAGCTTCTCCGACTACTCCGTGGGGTCGG
  AGTCAGACAGCTCCAAAGAAGAGACCATCTATGACACGATCCGGGCCACTGCGGAGAAGCCGGGCGGTGC
  CAGGACGGAGGAGAGCCAGGGCAACACGCTGGTGATCCGCGTGGTCATCCATGACCTGCAGCAGACGAAA
  TGCATTCGATTTAACCCGGATGCCACAGTGTGGGTTGCAAAGCAGCGGATCCTGTGTACATTAACCCAGA
  GTTTGAAAGATGTCCTGAACTACGGCCTGTTCCAGCCGGCCAGCAATGGGCGTGACGGCAAGTTCCTGGA
  TGAGGAGCGGCTCCTGCGCGAGTACCCACAGCCCGTGGGTGAGGGCGTTCCTTCCCTGGAGTTTCGATAC
  AAGAAGCGGGTGTATAAACAAGCCAGTCTCGATGAGAAACAGTTGGCCAAGCTCCACACGAAGACCAATC
  TGAAGAAATGCATGGATCACATTCAGCATCGCTTGGTGGAGAAGATCACCAAGATGCTGGACCGAGGCCT
  GGATCCCAATTTCCACGACCCGGAGACCGGAGAGACCCCCCTGACCTTAGCCGCTCAGCTGGACGACTCT
  GTGGAGGTCATCAAAGCTCTCAAAAATGGTGGAGCTCACCTGGACTTCCGTGCCAAAGATGGGATGACCG
  CCCTACACAAAGCTGCCCGAGCGAGGAACCAAGTTGCCCTGAAGACCCTTTTAGAGCTTGGTGCATCCCC
  AGATTATAAAGACAGTTACGGCCTCACCCCGCTGTATCACACAGCCATCGTCGGAGGTGATCCCTACTGC
  TGCGAGCTTCTCCTGCACGAACACGCCACTGTGTGCTGCAAAGATGAGAACGGCTGGCACGAGATCCACC
  AGGCCTGCAGGTACGGGCACGTGCAGCACCTGGAGCACCTGCTGTTCTACGGGGCAGACATGAGTGCCCA
  GAATGCCTCGGGGAACACGGCCTTGCACATCTGCGCCCTCTACAACCAGGACAGCTGTGCAAGAGTGCTT
  CTGTTTCGAGGCGGAAATAAGGAGTTAAAAAACTACAACAGCCAGACTCCATTTCAGGTGGCCATAATAG
  CAGGCAACTTTGAGCTGGCAGAATACATCAAGAACCACAAGGAAACAGACATTGTGCCCTTCCGAGAGGC
  CCCGGCGTACTCCAACCGCCGGCGGCGGCCCCCCAACACGCTGGCCGCCCCCCGGGTCCTGCTGCGCTCC
  AACAGTGACAACAACCTCAATGCCAGCGCTCCCGACTGGGCCGTCTGCTCCACGGCCACCTCGCACCGCA
  GCCTGTCACCCCAGCTGCTGCAGCAGATGCCCAGCAAGCCCGAGGGGGCCGCGAAGACCATTGGGAGCTA
  CGTGCCCGGGCCCCGCAGCCGGTCCCCATCGCTCAACAGGCTGGGCGGCGCAGGCGAGGACGGCAAGAGG
  CCGCAGCCTCTCTGGCATGTCGGGTCGCCTTTTGCTCTTGGTGCCAACAAGGACTCACTCTCGGCCTTCG
  AGTACCCGGGGCCCAAGCGGAAGCTCTACAGTGCCGTGCCCGGGAGGCTCTTCGTCGCTGTCAAGCCATA
  CCAACCCCAAGTGGACGGCGAGATCCCCCTTCACCGCGGTGACAGGGTCAAAGTTCTGAGCATCGGTGAA
  GGGGGCTTCTGGGAAGGCAGCGCCCGCGGCCACATCGGATGGTTTCCGGCGGAGTGCGTGGAGGAGGTCC
  AGTGTAAGCCCAGGGACAGCCAGGCAGAAACCCGCGCTGACCGCAGCAAGAAGCTTTTCAGGCACTACAC
  CGTGGGCTCCTATGACAGCTTCGACACTTCCAGTGACTGCATTATTGAGGAGAAGACGGTGGTCCTGCAG
  AAAAAAGACAATGAGGGCTTTGGATTCGTGCTTCGAGGGGCCAAAGCTGACACACCCATTGAAGAATTCA
  CACCAACACCGGCTTTCCCAGCCCTACAGTACCTGGAGTCCGTGGATGAAGGTGGGGTGGCGTGGCAAGC
  CGGACTAAGGACCGGGGACTTCTTGATTGAGGTTAACAATGAGAATGTTGTCAAAGTCGGCCACAGGCAG
  GTGGTGAACATGATCCGGCAGGGAGGGAATCACCTGGTCCTTAAGGTGGTCACGGTGACCAGGAATCTGG
  ACCCCGACGACACCGCCAGGAAGAAAGCTCCCCCGCCTCCAAAGCGGGCACCGACCACAGCCCTCACCCT
  GCGCTCCAAGTCCATGACCTCGGAGCTGGAGGAGCTCGTGGATAAAGCCTCGGTCCGGAAGAAGAAGGAT
  AAACCCGAGGAGATAGTCCCGGCCTCCAAGCCCTCCCGCGCTGCTGAGAACATGGCTGTGGAACCGAGGG
  TGGCGACCATCAAGCAGCGGCCCAGCAGCCGGTGCTTCCCGGCGGGCTCAGACATGAACTCTGTGTACGA
  ACGCCAAGGAATCGCCGTGATGACGCCCACTGTTCCTGGGAGCCCAAAAGCCCCGTTTCTGGGCATCCCT
  CGAGGTACGATGCGAAGGCAGAAATCAATAGACAGCAGAATCTTTCTATCAGGAATAACAGAGGAAGAGC
  GGCAGTTTCTGGCTCCTCCAATGCTGAAGTTCACCAGAAGCCTGTCCATGCCGGACACCTCTGAGGACAT
  CCCCCCTCCACCGCAGTCTGTGCCCCCGTCCCCACCACCACCTTCCCCAACCACTTACAACTGCCCCAAG
  TCCCCAACTCCAAGAGTCTACGGGACGATTAAGCCTGCGTTCAATCAGAATTCTGCCGCCAAGGTGTCCC
  CCGCCACCAGGTCCGACACCGTGGCCACCATGATGAGGGAGAAGGGGATGTACTTCAGGAGAGAGCTGGA
  CCGCTACTCCTTGGACTCTGAAGACCTCTACAGTCGGAATGCCGGCCCGCAAGCCAACTTCCGCAACAAG
  AGAGGCCAGATGCCAGAAAACCCATACTCAGAGGTGGGGAAGATCGCCAGCAAAGCCGTCTACGTCCCCG
  CCAAGCCCGCCAGGCGGAAGGGGATGCTGGTGAAGCAGTCCAACGTGGAGGACAGCCCCGAGAAGACGTG
  CTCCATCCCTATCCCGACCATCATCGTGAAGGAGCCGTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGC
  AGCAGCATGGAGATCGACCCCCAGGCCCCGGAGCCACCGAGCCAGCTGCGGCCTGACGAAAGCCTGACCG
  TCAGCAGCCCCTTTGCCGCCGCCATCGCCGGAGCCGTCCGCGACCGTGAGAAGCGGCTGGAAGCCAGGAG
  GAACTCCCCGGCCTTCCTCTCCACAGACCTGGGGGATGAGGATGTGGGCCTGGGGCCACCCGCCCCCAGG
  ACGCGGCCCTCCATGTTCCCCGAGGAGGGGGATTTTGCTGACGAGGACAGCGCTGAGCAGCTGTCATCCC
  CCATGCCGAGTGCCACGCCCAGGGAGCCCGAAAACCATTTCGTGGGTGGCGCCGAGGCCAGTGCTCCGGG
  TGAGGCTGGGAGGCCGCTGAATTCCACGTCCAAAGCCCAGGGGCCCGAGAGCAGCCCAGCAGTGCCCTCC
  GCGAGCAGCGGCACAGCCGGCCCCGGGAATTATGTCCACCCACTCACAGGGCGGCTGCTTGATCCCAGCT
  CCCCGCTGGCCCTGGCACTCTCCGCAAGGGACCGAGCCATGAAGGAGTCTCAACAGGGACCCAAAGGGGA
  GGCCCCCAAGGCCGACCTCAACAAACCTCTTTACATTGATACCAAAATGCGGCCCAGCCTGGATGCCGGC
  TTCCCTACGGTCACCAGGCAGAACACCCGGGGACCCCTGAGGCGGCAGGAGACGGAGAACAAGTACGAGA
  CCGACCTGGGCCGAGACCGGAAAGGCGATGACAAGAAGAACATGCTGATCGACATCATGGACACGTCCCA
  GCAGAAGTCGGCTGGCCTGCTGATGGTGCACACCGTGGACGCCACTAAGCTGGACAACGCCCTGCAGGAA
  GAGGACGAGAAGGCAGAGGTGGAGATGAAGCCAGACAGCTCGCCGTCCGAGGTGCCAGAAGGTGTTTCCG
  AAACCGAAGGTGCTTTACAGATCTCCGCTGCCCCCGAGCCCACCACCGTGCCCGGCAGAACCATCGTCGC
  GGTGGGCTCCATGGAAGAGGCGGTGATTTTGCCATTCCGCATCCCTCCTCCCCCTCTGGCATCCGTGGAC
  TTGGATGAGGATTTTATTTTTACAGAGCCATTGCCTCCTCCCCTGGAATTTGCAAATAGTTTTGATATCC
  CCGATGACCGGGCAGCTTCTGTCCCGGCTCTCTCAGACTTAGTGAAGCAGAAGAAAAGCGACACCCCTCA
  GTCCCCTTCGTTGAACTCCAGCCAACCAACCAACTCTGCAGACAGCAAGAAGCCAGCCAGTCTTTCAAAC
  TGTCTGCCTGCCTCATTCCTGCCACCCCCTGAAAGCTTTGACGCCGTCGCCGACTCTGGGATCGAGGAGG
  TGGACAGCCGGAGTAGCAGCGACCACCACCTCGAGACGACCAGCACTATCTCCACCGTGTCTAGCATCTC
  CACCCTGTCTTCCGAAGGTGGAGAGAATGTGGACACCTGCACAGTCTATGCAGATGGGCAAGCATTTATG
  GTTGACAAACCCCCAGTACCTCCTAAGCCAAAAATGAAGCCCATCATTCACAAAAGCAATGCACTTTATC
  AAGACGCGCTCGTGGAAGAAGATGTAGATAGCTTTGTTATCCCCCCGCCCGCTCCCCCGCCCCCGCCGGG
  CAGTGCCCAGCCTGGGATGGCCAAGGTTCTCCAGCCAAGGACCTCCAAGTTGTGGGGCGACGTCACAGAG
  ATCAAAAGCCCGATTCTCTCAGGCCCAAAGGCAAACGTTATTAGTGAATTGAACTCTATCCTACAGCAAA
  TGAACCGAGAGAAATTGGCAAAGCCGGGGGAAGGACTGGATTCACCAATGGGAGCCAAGTCCGCCAGCCT
  CGCTCCAAGAAGCCCGGAGATCATGAGCACCATCTCAGGTACACGGAGCACGACGGTCACCTTCACTGTT
  CGCCCCGGCACCTCCCAGCCCATCACCCTGCAGAGCCGGCCCCCCGACTATGAAAGCAGGACCTCAGGAA
  CAAGACGTGCCCCAAGCCCTGTGGTCTCGCCAACAGAGATGAACAAAGAGACCCTGCCCGCCCCCCTGTC
  TGCTGCCACCGCCTCTCCTTCTCCCGCTCTCTCAGATGTCTTTAGCCTTCCAAGCCAGCCCCCTTCTGGG
  GATCTATTTGGCTTGAACCCAGCGGGACGCAGTAGGTCGCCATCCCCCTCGATACTGCAACAGCCAATCT
  CAAATAAGCCTTTTACAACTAAACCTGTCCACCTGTGGACTAAACCAGATGTGGCCGATTGGCTGGAAAG
  TCTAAACTTGGGTGAACATAAAGAGGCCTTCATGGACAATGAGATCGATGGCAGTCACTTACCAAACCTG
  CAGAAGGAGGACCTCATCGATCTTGGGGTAACTCGAGTCGGGCACAGAATGAACATAGAAAGGGCTTTGA
  AACAGCTGCTGGACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229453 representing NM_012309
  Red=Cloning site Green=Tags(s)
  
  MPRSPTSSEDEMAQSFSDYSVGSESDSSKEETIYDTIRATAEKPGGARTEESQGNTLVIRVVIHDLQQTK
  CIRFNPDATVWVAKQRILCTLTQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGEGVPSLEFRY
  KKRVYKQASLDEKQLAKLHTKTNLKKCMDHIQHRLVEKITKMLDRGLDPNFHDPETGETPLTLAAQLDDS
  VEVIKALKNGGAHLDFRAKDGMTALHKAARARNQVALKTLLELGASPDYKDSYGLTPLYHTAIVGGDPYC
  CELLLHEHATVCCKDENGWHEIHQACRYGHVQHLEHLLFYGADMSAQNASGNTALHICALYNQDSCARVL
  LFRGGNKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDIVPFREAPAYSNRRRRPPNTLAAPRVLLRS
  NSDNNLNASAPDWAVCSTATSHRSLSPQLLQQMPSKPEGAAKTIGSYVPGPRSRSPSLNRLGGAGEDGKR
  PQPLWHVGSPFALGANKDSLSAFEYPGPKRKLYSAVPGRLFVAVKPYQPQVDGEIPLHRGDRVKVLSIGE
  GGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDTSSDCIIEEKTVVLQ
  KKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQ
  VVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELVDKASVRKKKD
  KPEEIVPASKPSRAAENMAVEPRVATIKQRPSSRCFPAGSDMNSVYERQGIAVMTPTVPGSPKAPFLGIP
  RGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPK
  SPTPRVYGTIKPAFNQNSAAKVSPATRSDTVATMMREKGMYFRRELDRYSLDSEDLYSRNAGPQANFRNK
  RGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQG
  SSMEIDPQAPEPPSQLRPDESLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPR
  TRPSMFPEEGDFADEDSAEQLSSPMPSATPREPENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPS
  ASSGTAGPGNYVHPLTGRLLDPSSPLALALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRPSLDAG
  FPTVTRQNTRGPLRRQETENKYETDLGRDRKGDDKKNMLIDIMDTSQQKSAGLLMVHTVDATKLDNALQE
  EDEKAEVEMKPDSSPSEVPEGVSETEGALQISAAPEPTTVPGRTIVAVGSMEEAVILPFRIPPPPLASVD
  LDEDFIFTEPLPPPLEFANSFDIPDDRAASVPALSDLVKQKKSDTPQSPSLNSSQPTNSADSKKPASLSN
  CLPASFLPPPESFDAVADSGIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGENVDTCTVYADGQAFM
  VDKPPVPPKPKMKPIIHKSNALYQDALVEEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTE
  IKSPILSGPKANVISELNSILQQMNREKLAKPGEGLDSPMGAKSASLAPRSPEIMSTISGTRSTTVTFTV
  RPGTSQPITLQSRPPDYESRTSGTRRAPSPVVSPTEMNKETLPAPLSAATASPSPALSDVFSLPSQPPSG
  DLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNL
  QKEDLIDLGVTRVGHRMNIERALKQLLDR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012309
• ORF Size: 5547 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012309.3, NP_036441.2
• RefSeq Size: 10641
• RefSeq ORF: 5550
• Locus ID: 22941
• Gene Summary: This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]