ZFYVE27 (NM_001174120) Human Tagged ORF Clone

CAT#: RG229877

ZFYVE27 (GFP-tagged) - Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZFYVE27
• Synonyms: PROTRUDIN; SPG33
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229877 representing NM_001174120
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGACATCAGAACGTGAGGGGAGTGGGCCGGAGCTGAGCCCCAGCGTGATGCCCGAGGCTCCCCTGG
  AGTCTCCACCTTTTCCTACCAAGTCCCCAGCGTTTGACCTTTTCAACTTGGTTCTCTCCTACAAGAGGCT
  GGAGATCTACCTGGAACCCTTGAAGGATGCAGGTGATGGTGTTCGATACTTGCTCAGCTTGATCCAGCTG
  GAGGCCTTCCTGAGCCGCCTGTGCTGCACATGTGAAGCCGCCTACCGCGTGCTGCACTGGGAGAACCCCG
  TCGTGTCCTCACAGTTCTATGGGGCTCTTCTGGGCACAGTCTGCATGCTGTATTTGCTGCCACTCTGCTG
  GGTTCTCACCCTTTTAAACAGCACGCTCTTTCTGGGGAATGTGGAGTTCTTCCGAGTTGTGTCTGAGTAC
  AGGGCATCTCTGCAGCAGAGGATGAACCCAAAGCAGGAAGAGCATGCCTTTGAGAGTCCTCCACCACCAG
  ATGTTGGGGGGAAGGATGGTCTGATGGACAGCACGCCTGCCCTCACACCCACGGAGGACCTCACACCGGG
  CAGCGTGGAGGAGGCTGAGGAGGCTGAGCCAGATGAAGAGTTTAAAGATGCGATTGAGGAGGATGATGAG
  GGCGCCCCGTGCCCAGCAGAGGATGAGCTGGCCCTGCAGGACAACGGGTTCCTGAGCAAGAATGAGGTGC
  TGCGCAGCAAGGTGTCTCGGCTCACGGAGCGGCTCCGCAAGCGCTACCCCACCAACAACTTCGGGAACTG
  CACGGGCTGCTCGGCCACCTTCTCAGTGCTGAAGAAGAGGCGGAGCTGCAGTAATTGTGGAAACAGCTTC
  TGCTCTCGATGCTGCTCCTTCAAGGTGCCCAAGTCCTCCATGGGGGCCACAGCCCCTGAAGCCCAGAGGG
  AGACTGTGTTTGTGTGTGCCTCGTGTAACCAGACCTTGAGCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229877 representing NM_001174120
  Red=Cloning site Green=Tags(s)
  
  MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLSLIQL
  EAFLSRLCCTCEAAYRVLHWENPVVSSQFYGALLGTVCMLYLLPLCWVLTLLNSTLFLGNVEFFRVVSEY
  RASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEEDDE
  GAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSF
  CSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174120
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001174120.1, NP_001167591.1
• RefSeq Size: 2776
• RefSeq ORF: 957
• Locus ID: 118813
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010]