ZIC4 (NM_001168378) Human Tagged ORF Clone

CAT#: RG229971

ZIC4 (GFP-tagged) - Human Zic family member 4 (ZIC4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZIC4
• Synonyms: FLJ42609; FLJ45833
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229971 representing NM_001168378
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCCTTCTGTAGGAATGCAAAGTTAGGCCTCCTTTCTTTCCTGATTTGTGCTGGAAGGAGCTTAT
  TACCTTTGGGGAAGAATGCCTTTTGGTTAGAAAGAATTGAAAATAATAATACCCTCTGGGAAAAGGCTCA
  AAGTCAGAAAATGAGATACAAGACATCCTTGGTGATGAGGAAACGATTACGGCTTTACCGAAACACTCTT
  AAAGAGTCAAGTAGCAGCTCTGGACACCATGGCCCCCAGCTCACCGCCGCCTCCAGCCCCTCGGTGTTCC
  CGGGCCTCCACGAGGAGCCTCCCCAGGCCTCCCCCAGCCGTCCTTTGAATGGACTCCTGCGTCTGGGGCT
  CCCTGGAGACATGTACGCGCGGCCGGAGCCCTTCCCGCCAGGGCCTGCGGCCCGCAGCGACGCCCTGGCA
  GCTGCCGCAGCCCTGCATGGCTACGGGGGCATGAACCTGACGGTGAACCTCGCTGCGCCCCACGGTCCTG
  GCGCTTTCTTCCGCTACATGCGCCAGCCCATCAAACAGGAGCTCATCTGCAAGTGGCTGGCGGCCGACGG
  CACCGCGACCCCGAGCCTCTGCTCCAAAACTTTCAGCACCATGCACGAGCTGGTCACGCACGTCACCGTG
  GAGCACGTCGGCGGCCCGGAACAGGCCAACCACATTTGCTTCTGGGAGGAGTGTCCGCGCCAGGGAAAGC
  CCTTCAAAGCCAAATACAAACTTGTAAATCACATCCGCGTGCACACGGGCGAGAAGCCCTTCCCTTGTCC
  TTTCCCGGGGTGTGGGAAGGTCTTTGCTAGATCAGAAAATCTCAAAATACACAAACGAACTCACACAGGC
  GAGAAGCCCTTCAGATGCGAGTTCGAGGGCTGCGAGCGGCGCTTCGCCAACAGCAGCGACCGTAAGAAGC
  ATTCGCACGTGCACACTAGCGACAAGCCATACACGTGCAAGGTGCGGGGCTGCGACAAGTGCTACACGCA
  CCCCAGCTCGCTGCGTAAGCACATGAAGGTGCACGGGCGCTCGCCGCCGCCCAGCTCTGGCTACGATTCG
  GCTACACCGTCTGCCCTCGTGTCGCCCTCGTCGGACTGCGGCCACAAGTCCCAGGTGGCCTCCTCGGCGG
  CGGTGGCGGCGCGTACCGCCGACTTGAGCGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229971 representing NM_001168378
  Red=Cloning site Green=Tags(s)
  
  MPPFCRNAKLGLLSFLICAGRSLLPLGKNAFWLERIENNNTLWEKAQSQKMRYKTSLVMRKRLRLYRNTL
  KESSSSSGHHGPQLTAASSPSVFPGLHEEPPQASPSRPLNGLLRLGLPGDMYARPEPFPPGPAARSDALA
  AAAALHGYGGMNLTVNLAAPHGPGAFFRYMRQPIKQELICKWLAADGTATPSLCSKTFSTMHELVTHVTV
  EHVGGPEQANHICFWEECPRQGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTG
  EKPFRCEFEGCERRFANSSDRKKHSHVHTSDKPYTCKVRGCDKCYTHPSSLRKHMKVHGRSPPPSSGYDS
  ATPSALVSPSSDCGHKSQVASSAAVAARTADLSE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168378
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001168378.1, NP_001161850.1
• RefSeq Size: 4152
• RefSeq ORF: 1155
• Locus ID: 84107
• Gene Summary: This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation, which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Dec 2009]