CUG BP1 (CELF1) (NM_001172639) Human Tagged ORF Clone

CAT#: RG230208

CELF1 (GFP-tagged) - Human CUGBP, Elav-like family member 1 (CELF1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CELF1
• Synonyms: BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP; hNab50; NAB50; NAPOR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230208 representing NM_001172639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGTTTAAGTTGGATTTCCTTCCAGAAATGATGGTGGATCATTGCTCTTTGAATTCCAGTCCCG
  TCTCAAAGAAAATGAACGGCACCCTGGACCACCCAGACCAACCAGATCTTGATGCTATCAAGATGTTTGT
  GGGCCAGGTTCCAAGGACCTGGTCTGAAAAGGACTTGCGGGAACTCTTCGAACAGTATGGTGCTGTGTAT
  GAAATCAACGTCCTAAGGGATAGGAGCCAAAACCCGCCTCAGAGCAAAGGGTGCTGTTTTGTTACATTTT
  ACACCCGTAAAGCTGCATTAGAAGCTCAGAATGCTCTTCACAACATGAAAGTCCTCCCAGGGATGCATCA
  CCCTATACAGATGAAACCTGCTGACAGTGAGAAGAACAATGTGGAAGACAGGAAGCTGTTTATTGGTATG
  ATTTCCAAGAAGTGCACTGAAAATGACATCCGAGTCATGTTCTCTTCGTTTGGACAGATTGAAGAATGCC
  GGATATTGCGGGGACCTGATGGCCTGAGCCGAGGTTGTGCATTTGTGACTTTTACAACAAGAGCCATGGC
  ACAGACGGCTATCAAGGCAATGCACCAAGCACAGACCATGGAGGGTTGCTCATCACCCATGGTGGTAAAA
  TTTGCTGATACACAGAAGGACAAAGAACAGAAGAGAATGGCCCAGCAGCTCCAGCAGCAGATGCAGCAAA
  TCAGCGCAGCATCTGTGTGGGGAAACCTTGCTGGTCTAAATACTCTTGGACCCCAGTATTTAGCACTTTA
  TTTGCAGCTCCTTCAGCAGACTGCCTCCTCTGGGAACCTCAACACCCTGAGCAGCCTCCACCCAATGGGA
  GGGTTGAATGCAATGCAGTTACAGAATTTGGCTGCACTAGCTGCTGCAGCTAGTGCAGCTCAGAACACAC
  CAAGTGGTACCAATGCTCTCACTACATCCAGCAGTCCCCTCAGCGTGCTCACTAGTTCAGGGTCCTCACC
  TAGCTCTAGCAGCAGTAATTCTGTCAACCCCATAGCCTCACTTGGAGCCCTGCAGACATTAGCTGGAGCA
  ACGGCTGGCCTCAATGTTGGCTCTTTGGCAGGAATGGCTGCTTTAAATGGTGGCCTGGGCAGCAGTGGCC
  TTTCCAATGGCACCGGGAGCACCATGGAGGCCCTCACTCAGGCCTACTCGGGTATCCAGCAATATGCTGC
  TGCTGCGCTCCCCACTCTGTACAACCAGAATCTTCTGACACAGCAGAGTATTGGTGCTGCTGGAAGCCAG
  AAGGAAGGTCCAGAGGGAGCCAACCTGTTCATCTACCACCTGCCCCAGGAGTTTGGTGATCAGGACCTGC
  TGCAGATGTTTATGCCCTTTGGGAATGTCGTGTCTGCCAAGGTTTTCATAGACAAGCAGACAAACCTGAG
  CAAGTGTTTTGGTTTTGTAAGTTACGACAATCCTGTTTCGGCCCAAGCTGCCATCCAGTCCATGAACGGC
  TTTCAGATTGGCATGAAGCGGCTTAAAGTGCAGCTCAAACGTTCGAAGAATGACAGCAAGCCCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230208 representing NM_001172639
  Red=Cloning site Green=Tags(s)
  
  MAAFKLDFLPEMMVDHCSLNSSPVSKKMNGTLDHPDQPDLDAIKMFVGQVPRTWSEKDLRELFEQYGAVY
  EINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNMKVLPGMHHPIQMKPADSEKNNVEDRKLFIGM
  ISKKCTENDIRVMFSSFGQIEECRILRGPDGLSRGCAFVTFTTRAMAQTAIKAMHQAQTMEGCSSPMVVK
  FADTQKDKEQKRMAQQLQQQMQQISAASVWGNLAGLNTLGPQYLALYLQLLQQTASSGNLNTLSSLHPMG
  GLNAMQLQNLAALAAAASAAQNTPSGTNALTTSSSPLSVLTSSGSSPSSSSSNSVNPIASLGALQTLAGA
  TAGLNVGSLAGMAALNGGLGSSGLSNGTGSTMEALTQAYSGIQQYAAAALPTLYNQNLLTQQSIGAAGSQ
  KEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMNG
  FQIGMKRLKVQLKRSKNDSKPY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172639
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001172639.1, NP_001166110.1
• RefSeq Size: 7434
• RefSeq ORF: 1539
• Locus ID: 10658
• Protein Families: Druggable Genome
• Gene Summary: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]