HABP2 (NM_001177660) Human Tagged ORF Clone

CAT#: RG230243

HABP2 (GFP-tagged) - Human hyaluronan binding protein 2 (HABP2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HABP2
• Synonyms: FSAP; HABP; HGFAL; NMTC5; PHBP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230243 representing NM_001177660
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTATTGGAAAGCCTGGACCCAGACTGGACCCCTGACCAGTATGATTACAGCTACGAGGATTATA
  ATCAGGAAGAGAACACCAGTAGCACACTTACCCACGCTGAGAATCCTGACTGGTACTACACTGAGGACCA
  AGCTGATCCATGCCAGCCCAACCCCTGTGAACACGGTGGGGACTGCCTCGTCCATGGGAGCACCTTCACA
  TGCAGCTGCCTGGCTCCTTTCTCTGGGAATAAGTGTCAGAAAGTGCAAAATACGTGCAAGGACAACCCAT
  GTGGCCGGGGCCAATGTCTCATTACCCAGAGTCCTCCCTACTACCGCTGTGTCTGTAAACACCCTTACAC
  AGGTCCCAGCTGCTCCCAAGTGGTTCCTGTATGCAGGCCAAACCCCTGCCAGAATGGGGCTACCTGCTCC
  CGGCATAAGCGGAGATCCAAGTTCACCTGTGCCTGTCCCGACCAGTTCAAGGGGAAATTCTGTGAAATAG
  GTTCTGATGACTGCTATGTTGGCGATGGCTACTCTTACCGAGGGAAAATGAATAGGACAGTCAACCAGCA
  TGCGTGCCTTTACTGGAACTCCCACCTCCTCTTGCAGGAGAATTACAACATGTTTATGGAGGATGCTGAA
  ACCCATGGGATTGGGGAACACAATTTCTGCAGAAACCCAGATGCGGACGAAAAGCCCTGGTGCTTTATTA
  AAGTTACCAATGACAAGGTGAAATGGGAATACTGTGATGTCTCAGCCTGCTCAGCCCAGGACGTTGCCTA
  CCCAGAGGAAAGCCCCACTGAGCCATCAACCAAGCTTCCGGGGTTTGACTCCTGTGGAAAGACTGAGATA
  GCAGAGAGGAAGATCAAGAGAATCTATGGAGGCTTTAAGAGCACGGCGGGCAAGCACCCATGGCAGGCGT
  CCCTCCAGTCCTCGCTGCCTCTGACCATCTCCATGCCCCAGGGCCACTTCTGTGGTGGGGCGCTGATCCA
  CCCCTGCTGGGTGCTCACTGCTGCCCACTGCACCGACATAAAAACCAGACATCTAAAGGTGGTGCTAGGG
  GACCAGGACCTGAAGAAAGAAGAATTTCATGAGCAGAGCTTTAGGGTGGAGAAGATATTCAAGTACAGCC
  ACTACAATGAAAGAGATGAGATTCCCCACAATGATATTGCATTGCTCAAGTTAAAGCCAGTGGATGGTCA
  CTGTGCTCTAGAATCCAAATACGTGAAGACTGTGTGCTTGCCTGATGGGTCCTTTCCCTCTGGGAGTGAG
  TGCCACATCTCTGGCTGGGGTGTTACAGAAACAGGAAAAGGGTCCCGCCAGCTCCTGGATGCCAAAGTCA
  AGCTGATTGCCAACACTTTGTGCAACTCCCGCCAACTCTATGACCACATGATTGATGACAGTATGATCTG
  TGCAGGAAATCTTCAGAAACCTGGGCAAGACACCTGCCAGGGTGACTCTGGAGGCCCCCTGACCTGTGAG
  AAGGACGGCACCTACTACGTCTATGGGATAGTGAGCTGGGGCCTGGAGTGTGGGAAGAGGCCAGGGGTCT
  ACACCCAAGTTACCAAATTCCTGAATTGGATCAAAGCCACCATCAAAAGTGAAAGTGGCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230243 representing NM_001177660
  Red=Cloning site Green=Tags(s)
  
  MSLLESLDPDWTPDQYDYSYEDYNQEENTSSTLTHAENPDWYYTEDQADPCQPNPCEHGGDCLVHGSTFT
  CSCLAPFSGNKCQKVQNTCKDNPCGRGQCLITQSPPYYRCVCKHPYTGPSCSQVVPVCRPNPCQNGATCS
  RHKRRSKFTCACPDQFKGKFCEIGSDDCYVGDGYSYRGKMNRTVNQHACLYWNSHLLLQENYNMFMEDAE
  THGIGEHNFCRNPDADEKPWCFIKVTNDKVKWEYCDVSACSAQDVAYPEESPTEPSTKLPGFDSCGKTEI
  AERKIKRIYGGFKSTAGKHPWQASLQSSLPLTISMPQGHFCGGALIHPCWVLTAAHCTDIKTRHLKVVLG
  DQDLKKEEFHEQSFRVEKIFKYSHYNERDEIPHNDIALLKLKPVDGHCALESKYVKTVCLPDGSFPSGSE
  CHISGWGVTETGKGSRQLLDAKVKLIANTLCNSRQLYDHMIDDSMICAGNLQKPGQDTCQGDSGGPLTCE
  KDGTYYVYGIVSWGLECGKRPGVYTQVTKFLNWIKATIKSESGF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177660
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177660.1, NP_001171131.1
• RefSeq Size: 2978 bp
• RefSeq ORF: 1605 bp
• Locus ID: 3026
• Cytogenetics: 10q25.3
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: 'This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by hepatocytes and proteolytically processed to generate heavy and light chains that form the mature heterodimer. Further autoproteolysis leads to smaller, inactive peptides. This extracellular protease binds hyaluronic acid and may play a role in the coagulation and fibrinolysis systems. Mutations in this gene are associated with nonmedullary thyroid cancer and susceptibility to venous thromboembolism. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]'