Dynein intermediate chain 2 (DNAI2) (NM_001172810) Human Tagged ORF Clone

CAT#: RG230321

DNAI2 (GFP-tagged) - Human dynein, axonemal, intermediate chain 2 (DNAI2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DNAI2
• Synonyms: CILD9; DIC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230321 representing NM_001172810
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATTGTGTACGTGTACGTCAAGAAGCGCAGCGAGTTCGGGAAGCAGTGCAATTTCTCGGACCGCC
  AGGCCGAGCTGAACATCGACATCATGCCCAACCCTGAGCTGGCCGAGCAGTTCGTGGAGCGGAACCCAGT
  GGACACGGGCATCCAGTGCTCGATCAGCATGTCGGAACACGAGGCCAACTCAGAGCGGTTTGAGATGGAG
  ACCCGGGGAGTTAACCATGTCGAGGGGGGCTGGCCCAAGGACGTGAACCCCCTGGAGCTGGAGCAGACCA
  TCCGTTTCCGGAAGAAAGTGGAGAAAGATGAGAACTACGTTAACGCCATCATGCAGCTCGGCTCTATCAT
  GGAGCACTGCATCAAGCAGAACAATGCCATTGACATCTATGAAGAGTATTTCAATGACGAGGAGGCCATG
  GAAGTGATGGAGGAGGACCCTTCAGCTAAAACCATCAATGTGTTCAGGGACCCCCAGGAAATCAAGAGGG
  CTGCCACACACCTCTCCTGGCACCCCGATGGCAACAGGAAGTTGGCAGTGGCATACTCCTGCTTGGATTT
  TCAGCGGGCACCTGTGGGCATGAGCAGCGATTCATACATCTGGGACCTGGAAAACCCCAACAAGCCTGAA
  CTTGCTCTGAAGCCATCGTCTCCACTCGTGACGTTGGAGTTCAACCCCAAAGATTCCCACGTACTCCTGG
  GTGGCTGCTACAATGGACAGATAGCCTGCTGGGACACCCGAAAGGGCAGCCTGGTGGCGGAGCTATCCAC
  CATTGAGTCCAGCCACCGAGACCCTGTGTATGGCACCATCTGGCTGCAGTCGAAGACGGGCACCGAGTGC
  TTCTCAGCTTCCACGGATGGGCAGGTCATGTGGTGGGACATCCGAAAGATGAGCGAGCCCACTGAAGTTG
  TGATCTTGGACATCACCAAGAAGGAACAGTTGGAAAATGCCTTGGGGGCCATCTCCCTGGAGTTCGAATC
  TACTTTGCCCACCAAGTTCATGGTGGGGACCGAGCAGGGCATCGTCATCTCCTGCAACCGCAAGGCCAAG
  ACGTCAGCTGAAAAGATTGTGTGCACCTTCCCGGGCCATCATGGCCCCATCTACGCCCTCCAGAGAAACC
  CCTTCTACCCGAAGAACTTCCTGACGGTTGGCGACTGGACAGCCCGCATTTGGTCTGAAGACAGCCGGGA
  ATCGTCCATCATGTGGACCAAGTACCACATGGCTTACCTCACTGATGCTGCCTGGAGCCCCGTGAGGCCG
  ACCGTTTTCTTTACCACCAGGATGGACGGAACCCTGGATATCTGGGACTTCATGTTCGAGCAGTGCGATC
  CCACCCTCAGCTTGAAGGACAATGGGTGTCTCATCGCCTGCGGCTCCCAGCTGGGGACAACCACCCTGCT
  GGAGGTCTCGCCTGGGCTCTCTACCCTCCAGAGGAATGAGAAGAACGTAGCCTCTTCCATGTTTGAGCGT
  GAGACCCGGCGAGAGAAGATCCTGGAGGCCAGGCACCGGGAGATGCGGCTGAAGGAGAAGGGTAAGGCGG
  AGGGCAGGGATGAGGAGCAGACCGATGAGGAGCTGGCCGTAGACCTGGAGGCGCTGGTCAGCAAGGCCGA
  GGAGGAGTTCTTCGACATCATCTTCGCAGAGCTGAAGAAGAAGGAGGCAGACGCCATAAAGCTGACGCCA
  GTGCCTCAGCAACCAAGTCCAGAAGAAGACCAGGTGGTGGAGGAGGGAGAGGAAGCAGCGGGGGAAGAAG
  GGGATGAAGAAGTGGAAGAAGACTTAGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230321 representing NM_001172810
  Red=Cloning site Green=Tags(s)
  
  MEIVYVYVKKRSEFGKQCNFSDRQAELNIDIMPNPELAEQFVERNPVDTGIQCSISMSEHEANSERFEME
  TRGVNHVEGGWPKDVNPLELEQTIRFRKKVEKDENYVNAIMQLGSIMEHCIKQNNAIDIYEEYFNDEEAM
  EVMEEDPSAKTINVFRDPQEIKRAATHLSWHPDGNRKLAVAYSCLDFQRAPVGMSSDSYIWDLENPNKPE
  LALKPSSPLVTLEFNPKDSHVLLGGCYNGQIACWDTRKGSLVAELSTIESSHRDPVYGTIWLQSKTGTEC
  FSASTDGQVMWWDIRKMSEPTEVVILDITKKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAK
  TSAEKIVCTFPGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLTDAAWSPVRP
  TVFFTTRMDGTLDIWDFMFEQCDPTLSLKDNGCLIACGSQLGTTTLLEVSPGLSTLQRNEKNVASSMFER
  ETRREKILEARHREMRLKEKGKAEGRDEEQTDEELAVDLEALVSKAEEEFFDIIFAELKKKEADAIKLTP
  VPQQPSPEEDQVVEEGEEAAGEEGDEEVEEDLA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172810
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001172810.1, NP_001166281.1
• RefSeq Size: 2255
• RefSeq ORF: 1782
• Locus ID: 64446
• Protein Pathways: Huntington's disease
• Gene Summary: The protein encoded by this gene belongs to the dynein intermediate chain family, and is part of the dynein complex of respiratory cilia and sperm flagella. Mutations in this gene are associated with primary ciliary dyskinesia type 9. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2010]