ZNF300 (NM_001172831) Human Tagged ORF Clone

CAT#: RG230357

ZNF300 (GFP-tagged) - Human zinc finger protein 300 (ZNF300), transcript variant 1. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF300
• Synonyms: DKFZp686B24204
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230357 ORF sequence, codon optimized.
  Due to the complexity of NM_001172831, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCCAGCAGAAACATCCAGCAGCGGGCTGCTGGAAGAGCAAAAAATGATGAAGTCCCAGGGCCTGG
  TATCCTTTAAGGACGTGGCTGTTGACTTCACTCAAGAAGAATGGCAGCAGTTGGATCCTAGCCAGAGGAC
  CTTGTATAGGGATGTGATGCTGGAGAATTATAGTCACCTCGTGAGCATGGGGTATCCCGTCAGCAAGCCC
  GATGTCATTAGTAAATTGGAGCAGGGGGAGGAGCCTTGGATAATTAAGGGGGATATAAGCAACTGGATTT
  ATCCTGATGAGTACCAGGCGGACGGTCGACAAGATCGAAAGAGCAACCTCCATAATAGCCAATCTTGCAT
  ACTTGGGACCGTCTCCTTCCATCACAAGATCCTTAAAGGTGTGACTCGGGATGGCTCTCTGTGCTCAATT
  CTGAAGGTCTGTCAAGGCGATGGACAACTCCAGAGATTCCTGGAGAATCAGGACAAGCTCTTCCGGCAGG
  TGACCTTCGTCAACAGCAAGACAGTGACTGAGGCCAGTGGCCACAAGTACAATCCCCTGGGAAAGATCTT
  CCAGGAATGTATCGAGACGGATATTTCCATCCAGAGGTTTCACAAATACGATGCTTTCAAGAAGAACCTG
  AAGCCAAACATCGATCTGCCGTCATGTTATAAGAGCAACTCCCGCAAAAAACCTGATCAGTCCTTTGGCG
  GTGGGAAATCTTCAAGCCAGTCCGAACCTAATAGTAATTTGGAGAAGATCCACAACGGGGTGATCCCGTT
  CGACGATAACCAGTGCGGAAACGTTTTCAGAAATACACAGTCCTTGATTCAGTACCAAAACGTCGAGACA
  AAAGAAAAAAGCTGCGTGTGCGTTACGTGTGGCAAGGCGTTCGCTAAGAAGTCCCAGCTGATAGTCCATC
  AAAGAATTCATACAGGCAAGAAGCCCTATGACTGTGGAGCTTGTGGAAAAGCCTTCTCAGAAAAGTTTCA
  TCTTGTTGTGCATCAGAGAACACACACCGGAGAAAAACCCTACGACTGTAGTGAATGCGGGAAAGCCTTC
  TCTCAGAAAAGCAGCCTCATCATTCACCAGCGGGTGCACACAGGAGAGAAGCCCTATGAGTGTAGCGAGT
  GTGGAAAAGCGTTTAGCCAGAAATCCCCACTGATCATTCACCAGCGGATCCACACCGGCGAGAAACCCTA
  CGAATGCCGAGAATGTGGGAAAGCCTTCTCACAAAAGAGCCAGCTGATCATCCACCACAGGGCTCATACG
  GGTGAAAAACCTTACGAGTGCACAGAATGTGGTAAAGCATTTTGCGAAAAAAGTCACCTTATCATACACA
  AAAGAATTCATACCGGCGAGAAACCCTACAAGTGCGCTCAATGCGAGGAGGCGTTTAGCCGGAAGACCGA
  ACTGATCACACATCAGCTCGTACACACAGGCGAGAAGCCATATGAGTGCACCGAATGCGGCAAAACATTT
  AGCAGGAAATCACAGCTCATCATTCATCAGCGAACCCACACTGGAGAAAAACCTTACAAATGCTCCGAGT
  GTGGCAAGGCTTTTTGTCAGAAGTCTCACCTCATTGGCCACCAGAGGATACATACAGGTGAAAAACCCTA
  TATTTGCACCGAATGCGGTAAGGCCTTCAGTCAGAAGAGCCACCTGCCTGGGCACCAGAGGATCCACACT
  GGGGAGAAACCCTATATCTGTGCTGAATGTGGCAAAGCCTTTAGCCAGAAATCCGACCTGGTGCTGCACC
  AGCGGATTCATACCGGCGAAAGACCTTACCAGTGCGCCATCTGTGGAAAGGCTTTTATACAGAAGAGCCA
  GCTCACTGTGCATCAGCGCATACACACCGTTGTCAAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230357 representing NM_001172831
  Red=Cloning site Green=Tags(s)
  
  MVPAETSSSGLLEEQKMMKSQGLVSFKDVAVDFTQEEWQQLDPSQRTLYRDVMLENYSHLVSMGYPVSKP
  DVISKLEQGEEPWIIKGDISNWIYPDEYQADGRQDRKSNLHNSQSCILGTVSFHHKILKGVTRDGSLCSI
  LKVCQGDGQLQRFLENQDKLFRQVTFVNSKTVTEASGHKYNPLGKIFQECIETDISIQRFHKYDAFKKNL
  KPNIDLPSCYKSNSRKKPDQSFGGGKSSSQSEPNSNLEKIHNGVIPFDDNQCGNVFRNTQSLIQYQNVET
  KEKSCVCVTCGKAFAKKSQLIVHQRIHTGKKPYDCGACGKAFSEKFHLVVHQRTHTGEKPYDCSECGKAF
  SQKSSLIIHQRVHTGEKPYECSECGKAFSQKSPLIIHQRIHTGEKPYECRECGKAFSQKSQLIIHHRAHT
  GEKPYECTECGKAFCEKSHLIIHKRIHTGEKPYKCAQCEEAFSRKTELITHQLVHTGEKPYECTECGKTF
  SRKSQLIIHQRTHTGEKPYKCSECGKAFCQKSHLIGHQRIHTGEKPYICTECGKAFSQKSHLPGHQRIHT
  GEKPYICAECGKAFSQKSDLVLHQRIHTGERPYQCAICGKAFIQKSQLTVHQRIHTVVKS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172831
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001172831.1, NP_001166302.1
• RefSeq Size: 3337
• RefSeq ORF: 1863
• Locus ID: 91975
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene is a C2H2-type zinc finger DNA binding protein and likely transcriptional regulator. The function of this protein is not yet known. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]