SMPD4 (NM_001171083) Human Tagged ORF Clone

CAT#: RG230482

SMPD4 (GFP-tagged) - Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SMPD4
• Synonyms: NET13; NSMASE-3; NSMASE3; SKNY
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230482 representing NM_001171083
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACTTTCGGCGCCGTGGCGGAATGGCGGCTTCCATCTCTGAGGCGAGCGACGCTATGGATCCCAC
  AGTGGTTTGCTAAGAAGGCCATTTTCAACTCTCCACTGGAGGCTGCTATGGCGTTCCCTCACCTGCAGCA
  GCCCAGCTTTCTACTGGCTAGCCTGAAAGCTGACTCTATAAATAAGCCCTTTGCACAGCAGTGCCAAGAC
  TTGGTTAAAGTCATTGAGGACTTTCCAGCAAAGGGTCCTGTGAAGGCGTCCATCCAGGAGTGCATCCTCC
  CTGACAGTCCTCTGTACCACAACAAGGTCCAGTTCACCCCTACTGGGGGCCTTGGTCTGAACTTGGCCCT
  GAATCCGTTCGAGTATTACATATTCTTCTTTGCCTTGAGCCTCATCACTCAGAAGCCACTTCCTGTGTCC
  CTCCACGTCCGTACTTCAGACTGTGCCTATTTCATCCTGGTGGACAGGTACCTGTCATGGTTCCTGCCCA
  CCGAAGGCAGTGTGCCCCCACCACTCTCCTCCAGCCCAGGGGGGACCAGCCCCTCACCACCTCCCAGGAC
  ACCAGCCATACCCTTTGCTTCCTATGGCCTCCACCACACTAGCCTCCTAAAGCGACACATCTCTCATCAG
  ACGTCTGTGAATGCAGACCCCGCCTCCCACGAGATCTGGAGGTCAGAAACTCTGCTCCAGGTTTTTGTTG
  AAATGTGGCTTCATCACTATTCCTTGGAGATGTATCAAAAAATGCAGTCCCCTCATGCCAAGGAGTCGTT
  CACGCCTACTGAGGAGCATGTGTTGGTGGTGCGCCTGCTGCTGAAGCACCTGCACGCCTTTGCCAACAGC
  CTGAAGCCAGAGCAGGCCTCACCCTCCGCCCACTCCCACGCCACCAGCCCCCTGGAGGAGTTCAAACGGG
  CTGCTGTCCCGAGGTTCGTCCAGCAGAAACTCTACCTCTTCTTGCAGCATTGCTTTGGCCACTGGCCCCT
  GGACGCATCGTTCAGAGCTGTCCTGGAGATGTGGCTGAGCTACCTGCAGCCGTGGCGGTACGCGCCTGAC
  AAGCAGGCTCCGGGCAGCGACTCCCAGCCCCGGTGTGTGTCGGAGAAATGGGCACCCTTTGTCCAGGAGA
  ACCTGCTGATGTACACCAAGTTGTTTGTGGGCTTTCTGAACCGCGCGCTCCGCACAGACCTGGTCAGCCC
  CAAGCACGCGCTCATGGTGTTCCGAGTGGCCAAAGTCTTTGCCCAGCCCAACCTGGCTGAGATGATTCAG
  AAAGGTGAGCAGCTATTCCTGGAGCCAGAGCTGGTCATCCCCCACCGCCAGCACCGACTCTTCACGGCCC
  CCACATTCACTGGGAGCTTCCTGTCACCCTGGCCACCAGCGGTCACTGATGCCTCCTTCAAGGTGAAGAG
  CCACGTCTACAGCCTGGAGGGCCAGGACTGCAAGTACACCCCGATGTTTGGGCCCGAGGCCCGCACCCTG
  GTCCTGCGCCTCGCTCAGCTCATCACACAGGCCAAACACACAGCCAAGTCCATCTCCGACCAGTGTGCGG
  AGAGCCCGGCTGGCCACTCCTTCCTCTCATGGCTGGGCTTTAGCTCCATGGACACCAATGGCTCCTACAC
  AGCCAACGACCTGGACGAGATGGGGCAAGACAGTGTCCGGAAGACAGATGAATACCTGGAGAAGGCCCTG
  GAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCGCAGCTCAGGCAGTTCACACTCGCCTTGGGCACCA
  CCCAGGATGAGAATGGAAAAAAGCAACTCCCCGACTGCATCGTGGGTGAGGACGGACTCATCCTTACGCC
  CCTGGGGCGGTACCAGATCATCAATGGGCTGCGAAGGTTTGAAATTGAGTACCAGGGGGACCCGGAGCTG
  CAGCCCATCCGGAGCTATGAGATCGCCAGCTTGGTCCGCACACTCTTTAGGCTGTCGTCTGCCATCAACC
  ACAGATTTGCAGGACAGATGGCGGCTCTGTGTTCCCGGGATGACTTCCTCGGCAGCTTCTGTCGCTACCA
  CCTCACAGAACCTGGGCTGGCCAGCAGGCACCTGCTGAGCCCTGTGGGGCGGAGGCAGGTGGCCGGCCAC
  ACCCGCGGCCCCAGGCTCAGCCTGCGCTTCCTGGGCAGTTACCGGACGCTGGTCTCGCTGCTGCTGGCCT
  TCTTCGTGGCCTCTCTGTTCTGCGTCGGGCCCCTCCCATGCACGCTGCTGCTCACCCTGGGCTATGTCCT
  CTACGCCTCTGCCATGACACTGCTGACCGAGCGGGGGAAGCTGCACCAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230482 representing NM_001171083
  Red=Cloning site Green=Tags(s)
  
  MTTFGAVAEWRLPSLRRATLWIPQWFAKKAIFNSPLEAAMAFPHLQQPSFLLASLKADSINKPFAQQCQD
  LVKVIEDFPAKGPVKASIQECILPDSPLYHNKVQFTPTGGLGLNLALNPFEYYIFFFALSLITQKPLPVS
  LHVRTSDCAYFILVDRYLSWFLPTEGSVPPPLSSSPGGTSPSPPPRTPAIPFASYGLHHTSLLKRHISHQ
  TSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQKMQSPHAKESFTPTEEHVLVVRLLLKHLHAFANS
  LKPEQASPSAHSHATSPLEEFKRAAVPRFVQQKLYLFLQHCFGHWPLDASFRAVLEMWLSYLQPWRYAPD
  KQAPGSDSQPRCVSEKWAPFVQENLLMYTKLFVGFLNRALRTDLVSPKHALMVFRVAKVFAQPNLAEMIQ
  KGEQLFLEPELVIPHRQHRLFTAPTFTGSFLSPWPPAVTDASFKVKSHVYSLEGQDCKYTPMFGPEARTL
  VLRLAQLITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKAL
  EYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRRFEIEYQGDPEL
  QPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFLGSFCRYHLTEPGLASRHLLSPVGRRQVAGH
  TRGPRLSLRFLGSYRTLVSLLLAFFVASLFCVGPLPCTLLLTLGYVLYASAMTLLTERGKLHQP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171083
• ORF Size: 2292 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001171083.2, NP_001164554.1
• RefSeq Size: 3614
• RefSeq ORF: 2295
• Locus ID: 55627
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Gene Summary: The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. [provided by RefSeq, Mar 2017]