CLCN2 (NM_001171087) Human Tagged ORF Clone

CAT#: RG230559

CLCN2 (GFP-tagged) - Human chloride channel 2 (CLCN2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CLCN2
• Synonyms: CIC-2; clC-2; CLC2; ECA2; ECA3; EGI3; EGI11; EGMA; EJM6; EJM8; HALD2; LKPAT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230559 representing NM_001171087
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCGGCGGCGGAGGAAGGGATGGAGCCACGGGCGCTGCAGTACGAGCAGACCCTGATGTATG
  GCCGGTACACTCAGGACCTTGGGGCCTTTGCCAAAGAGGAAGCTGCTCGGATTCGCCTGGGAGGGCCTGA
  ACCCTGGAAAGGTCCCCCTTCCTCTCGGGCTGCCCCAGAGCTCTTGGAATATGGACGGAGCCGTTGCGCC
  CGATGCCGCGTCTGTTCTGTCCGCTGCCACAAGTTCCTAGTATCCAGGGTTGGTGAAGATTGGATCTTCC
  TGGTCCTGCTGGGGCTTCTCATGGCATTGGTCAGCTGGGTCATGGACTATGCCATTGCTGCCTGTCTGCA
  AGCCCAGCAGTGGATGTCCCGGGGCTTGAACACCAGCATCTTGCTCCAGTACCTGGCCTGGGTCACCTAC
  CCTGTTGTCCTCATCACTTTCTCAGCCGGATTCACACAGATCCTGGCCCCTCAGGCTGTCGGCTCTGGCA
  TCCCTGAGATGAAGACCATCTTGCGGGGAGTGGTGCTGAAAGAATACCTCACACTCAAGACCTTTATAGC
  TAAGGTCATTGGGCTGACCTGCGCCCTAGGCAGCGGGATGCCGCTTGGCAAAGAGGGCCCTTTTGTGCAT
  ATCGCAAGCATGTGTGCTGCCCTTCTCAGCAAGTTCCTCTCCCTCTTTGGGGGTATCTATGAGAATGAAT
  CCCGGAACACAGAGATGCTGGCTGCCGCCTGTGCCGTGGGGGTGGGCTGCTGCTTCGCGGCACCTATTGG
  AGGCGTCCTCTTCAGCATCGAGGTCACCTCCACCTTCTTTGCAGTGCGGAACTACTGGCGGGGCTTCTTC
  GCTGCCACCTTCAGTGCCTTCATCTTCCGGGTCTTGGCAGTCTGGAACCGGGATGAAGAGACTATTACAG
  CCCTCTTCAAAACCCGATTCCGGCTCGACTTCCCCTTTGACCTGCAGGAGCTGCCAGCCTTTGCTGTCAT
  TGGTATTGCTAGTGGCTTCGGTGGAGCCCTCTTTGTCTACCTGAACCGGAAGATTGTCCAGGTGATGCGG
  AAGCAGAAAACCATCAATCGCTTCCTCATGAGGAAACGCCTGCTCTTCCCGGCTCTGGTGACCCTGCTCA
  TCTCCACGCTGACCTTCCCCCCTGGCTTTGGACAGTTCATGGCTGGACAGCTCTCACAGAAAGAGACGCT
  GGTCACCCTGTTTGACAATCGGACGTGGGTCCGCCAGGGCCTGGTGGAGGAGCTAGAACCACCCAGCACC
  TCACAGGCCTGGAACCCACCACGTGCCAACGTCTTCCTCACCCTGGTCATCTTCATTCTCATGAAGTTCT
  GGATGTCTGCACTGGCCACCACCATCCCAGTTCCCTGTGGGGCCTTCATGCCTGTCTTTGTCATTGATGG
  AATTCATACGGACAGCAGCACCTACCGGATTGTGCCTGGGGGCTACGCTGTGGTCGGGGCAGCTGCGCTG
  GCAGGAGCGGTGACACACACAGTGTCCACGGCTGTGATCGTGTTCGAGCTCACAGGCCAGATTGCCCACA
  TCCTGCCTGTCATGATCGCCGTCATCCTGGCCAACGCTGTCGCCCAGAGTCTGCAGCCCTCCCTCTATGA
  CAGCATCATCCGAATCAAGAAACTGCCCTACCTGCCTGAGCTCGGCTGGGGCCGCCACCAGCAGTACCGG
  GTGCGTGTGGAGGACATCATGGTGCGGGATGTTCCCCATGTGGCCCTCAGCTGCACCTTCCGGGACCTGC
  GTTTGGCACTGCACAGGACCAAGGGCCGAATGCTGGCCCTAGTGGAGTCCCCTGAGTCCATGATTCTGCT
  GGGCTCCATCGAGCGTTCACAGGTGGTGGCATTGTTGGGGGCCCAGCTGAGCCCAGCCCGCCGGCGGCAG
  CACATGCAGGAGCGCAGAGCCACCCAGACCTCTCCACTATCTGATCAGGAGGGTCCCCCTACCCCTGAGG
  CTTCTGTCTGCTTCCAGGTGAACACAGAAGACTCAGCCTTCCCAGCAGCCCGGGGGGAGACCCACAAGCC
  CCTAAAGCCTGCACTCAAGAGGGGGCCCAGTGTCACCAGGAACCTCGGAGAGAGTCCCACAGGGAGCGCA
  GAGTCGGCAGGCATCGCCCTCCGGAGCCTCTTCTGTGGCAGTCCACCCCCTGAGGCTGCTTCGGAGAAGT
  TGGAATCCTGTGAGAAGCGCAAGCTGAAGCGTGTCCGAATCTCCCTGGCAAGTGACGCGGACCTGGAAGG
  CGAGATGAGCCCTGAAGAGATTCTGGAGTGGGAGGAGCAGCAACTAGATGAACCTGTCAACTTCAGTGAC
  TGCAAAATTGATCCTGCTCCCTTCCAGCTGGTGGAGCGGACCTCTTTGCACAAGACTCACACTATCTTCT
  CACTGCTGGGAGTGGACCATGCTTATGTCACCAGTATTGGCAGACTCATTGGAATCGTTACTCTAAAGGA
  GCTCCGGAAGGCCATCGAGGGCTCTGTCACAGCACAGGGTGTGAAAGTCCGGCCGCCCCTCGCCAGCTTC
  CGAGACAGTGCCACCAGCAGCAGTGACACGGAGACCACTGAGGTGCATGCACTCTGGGGGCCCCACTCCC
  GTCATGGCCTCCCCCGGGAGGGCAGCCCTTCCGACAGCGACGACAAATGCCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230559 representing NM_001171087
  Red=Cloning site Green=Tags(s)
  
  MAAAAAEEGMEPRALQYEQTLMYGRYTQDLGAFAKEEAARIRLGGPEPWKGPPSSRAAPELLEYGRSRCA
  RCRVCSVRCHKFLVSRVGEDWIFLVLLGLLMALVSWVMDYAIAACLQAQQWMSRGLNTSILLQYLAWVTY
  PVVLITFSAGFTQILAPQAVGSGIPEMKTILRGVVLKEYLTLKTFIAKVIGLTCALGSGMPLGKEGPFVH
  IASMCAALLSKFLSLFGGIYENESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFF
  AATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMR
  KQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEELEPPST
  SQAWNPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIDGIHTDSSTYRIVPGGYAVVGAAAL
  AGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYR
  VRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQ
  HMQERRATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAARGETHKPLKPALKRGPSVTRNLGESPTGSA
  ESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLASDADLEGEMSPEEILEWEEQQLDEPVNFSD
  CKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASF
  RDSATSSSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171087
• ORF Size: 2643 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171087.2, NP_001164558.1
• RefSeq Size: 3248 bp
• RefSeq ORF: 2646 bp
• Locus ID: 1181
• Cytogenetics: 3q27.1
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: 'This gene encodes a voltage-gated chloride channel. The encoded protein is a transmembrane protein that maintains chloride ion homeostasis in various cells. Defects in this gene may be a cause of certain epilepsies. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]'