NALP2 (NLRP2) (NM_001174082) Human Tagged ORF Clone

CAT#: RG230628

NLRP2 (GFP-tagged) - Human NLR family, pyrin domain containing 2 (NLRP2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NLRP2
• Synonyms: CLR19.9; NALP2; NBS1; PAN1; PYPAF2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230628 representing NM_001174082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCTTCGGCGCAGATGGGCTTCAACCTGCAGGCTCTCCTGGAGCAGCTCAGCCAGGATGAGTTGA
  GCAAGTTCAAGTATCTGATCACGACCTTCTCCCTGGCACACGAGCTCCAGAAGATCCCCCACAAGGAGGT
  AGACAAGGCTGATGGGAAGCAACTGGTAGAAATCCTCACCACCCATTGTGACAGCTACTGGGTGGAGATG
  GCGAGCCTCCAGGTCTTTGAAAAGATGCACCGAATGGATCTGTCTGAGAGAGCAAAGGATGAAGTCAGAG
  AAGCAGCTTTGAAATCCTTTAATAAAAGGAAGCCTCTATCATTAGGGATAACACGGAAAGAACGACCACC
  TCTAGACGTGGACGAAATGCTGGAGCGCTTCAAAACAGAAGCACAAGACAAAGACAATAGGTGCAGGTAT
  ATATTGAAGACGAAGTTCCGGGAGATGTGGAAGAGCTGGCCTGGAGATAGCAAAGAGGTCCAGGTTATGG
  CTGAGAGATACAAGATGCTGATCCCATTCAGCAACCCCAGGGTGCTTCCCGGGCCCTTCTCATACACGGT
  GGTGCTGTATGGTCCTGCAGGCCTTGGGAAAACCACGCTGGCCCAGAAACTAATGCTAGACTGGGCAGAG
  GACAACCTCATCCACAAATTCAAATATGCGTTCTACCTCAGCTGCAGGGAGCTCAGCCGCCTGGGCCCGT
  GCAGTTTTGCAGAGCTGGTCTTCAGGGACTGGCCTGAATTGCAGGATGACATTCCACACATCCTAGCCCA
  AGCACGGAAAATCTTGTTCGTGATTGACGGCTTTGATGAGCTGGGAGCCGCACCTGGGGCGCTGATCGAG
  GACATCTGCGGGGACTGGGAGAAGAAGAAGCCGGTGCCCGTCCTCCTGGGGAGTTTGCTGAACAGGGTGA
  TGTTACCCAAGGCCGCCCTGCTGGTCACCACGCGGCCCAGGGCCCTGAGGGACCTCCGGATCCTGGCGGA
  GGAGCCGATCTACATAAGGGTGGAGGGCTTCCTGGAGGAGGACAGGAGGGCCTATTTCCTGAGACACTTT
  GGAGACGAGGACCAAGCCATGCGTGCCTTTGAGCTAATGAGGAGCAACGCGGCCCTGTTCCAGCTGGGCT
  CGGCCCCCGCGGTGTGCTGGATCGTGTGCACGACTCTGAAGCTGCAGATGGAGAAGGGGGAGGACCCGGT
  CCCCACCTGCCTCACCCGCACGGGGCTGTTCCTGCGTTTCCTCTGCAGCCGGTTCCCGCAGGGCGCACAG
  CTGCGGGGCGCGCTGCGGACGCTGAGCCTCCTGGCCGCGCAGGGCCTGTGGGCGCAGACGTCCGTGCTTC
  ACCGAGAGGATCTGGAAAGGCTCGGGGTGCAGGAGTCCGACCTCCGTCTGTTCCTGGACGGAGACATCCT
  CCGCCAGGACAGAGTCTCCAAAGGCTGCTACTCCTTCATCCACCTCAGCTTCCAGCAGTTTCTCACTGCC
  CTGTTCTACACCCTGGAGAAGGAGGAGGAAGAGGATAGGGACGGCCACACCTGGGACATTGGGGACGTAC
  AGAAGCTGCTTTCCGGAGTAGAAAGACTCAGGAACCCCGACCTGATCCAAGCAGGCTACTACTCCTTTGG
  CCTCGCTAACGAGAAGAGAGCCAAGGAGTTGGAGGCCACTTTTGGCTGCCGGATGTCACCGGACATCAAA
  CAGGAATTGCTGCGATGCGACATAAGTTGTAAGGGTGGACATTCAACGGTGACAGACCTGCAGGAGCTCC
  TCGGCTGTCTGTACGAGTCTCAGGAGGAGGAGCTGGTGAAGGAGGTGATGGCTCAGTTCAAAGAAATATC
  CCTGCACTTAAATGCAGTAGACGTTGTGCCATCTTCATTCTGCGTCAAGCACTGTCGAAACCTGCAGAAA
  ATGTCACTGCAGGTAATAAAGGAGAATCTCCCGGAGAATGTCACTGCGTCTGAATCAGACGCCGAGGTTG
  AGAGATCCCAGGATGATCAGCACATGCTTCCTTTCTGGACGGACCTTTGTTCCATATTTGGATCAAATAA
  GGATCTGATGGGTCTAGCAATCAATGATAGCTTTCTCAGTGCCTCCCTAGTAAGGATCCTGTGTGAACAA
  ATAGCCTCTGACACCTGTCATCTCCAGAGAGTGGTGTTCAAAAACATTTCCCCAGCTGATGCTCATCGGA
  ACCTCTGCCTAGCTCTTCGAGGTCACAAGACTGTAACGTATCTGACCCTTCAAGGCAATGACCAGGATGA
  TATGTTTCCCGCATTGTGTGAGGTCTTGAGACATCCAGAATGTAACCTGCGATATCTCGGGTTGGTGTCT
  TGTTCCGCTACCACTCAGCAGTGGGCTGATCTCTCCTTGGCCCTTGAAGTCAACCAGTCCCTGACGTGCG
  TAAACCTCTCCGACAATGAGCTTCTGGATGAGGGTGCTAAGTTGCTGTACACAACTTTGAGACACCCCAA
  GTGCTTTCTGCAGAGGTTGTCGTTGGAAAACTGTCACCTTACAGAAGCCAATTGCAAGGACCTTGCTGCT
  GTGTTGGTTGTCAGCCGGGAGCTGACACACCTGTGCTTGGCCAAGAACCCCATTGGGAATACAGGGGTGA
  AGTTTCTGTGTGAGGGCTTGAGGTACCCCGAGTGTAAACTGCAGACCTTGGTGCTTTGGAACTGCGACAT
  AACTAGCGATGGCTGCTGCGATCTCACAAAGCTTCTCCAAGAAAAATCAAGCCTGTTGTGTTTGGATCTG
  GGGCTGAATCACATAGGAGTTAAGGGAATGAAGTTCCTGTGTGAGGCTTTGAGGAAACCACTGTGCAACT
  TGAGATGTCTGTGGTTGTGGGGATGTTCCATCCCTCCGTTCAGTTGTGAAGACCTCTGCTCTGCCCTCAG
  CTGCAACCAGAGCCTCGTCACTCTGGACCTGGGTCAGAATCCCTTGGGGTCTAGTGGAGTGAAGATGCTG
  TTTGAAACCTTGACATGTTCCAGTGGCACCCTCCGGACACTCAGGTTGAAAATCGATGACTTTAATGATG
  AACTCAATAAGCTGCTGGAAGAAATAGAAGAAAAAAACCCACAACTGATTATTGATACTGAGAAACATCA
  TCCCTGGGCAGAAAGGCCTTCTTCTCATGACTTCATGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230628 representing NM_001174082
  Red=Cloning site Green=Tags(s)
  
  MVSSAQMGFNLQALLEQLSQDELSKFKYLITTFSLAHELQKIPHKEVDKADGKQLVEILTTHCDSYWVEM
  ASLQVFEKMHRMDLSERAKDEVREAALKSFNKRKPLSLGITRKERPPLDVDEMLERFKTEAQDKDNRCRY
  ILKTKFREMWKSWPGDSKEVQVMAERYKMLIPFSNPRVLPGPFSYTVVLYGPAGLGKTTLAQKLMLDWAE
  DNLIHKFKYAFYLSCRELSRLGPCSFAELVFRDWPELQDDIPHILAQARKILFVIDGFDELGAAPGALIE
  DICGDWEKKKPVPVLLGSLLNRVMLPKAALLVTTRPRALRDLRILAEEPIYIRVEGFLEEDRRAYFLRHF
  GDEDQAMRAFELMRSNAALFQLGSAPAVCWIVCTTLKLQMEKGEDPVPTCLTRTGLFLRFLCSRFPQGAQ
  LRGALRTLSLLAAQGLWAQTSVLHREDLERLGVQESDLRLFLDGDILRQDRVSKGCYSFIHLSFQQFLTA
  LFYTLEKEEEEDRDGHTWDIGDVQKLLSGVERLRNPDLIQAGYYSFGLANEKRAKELEATFGCRMSPDIK
  QELLRCDISCKGGHSTVTDLQELLGCLYESQEEELVKEVMAQFKEISLHLNAVDVVPSSFCVKHCRNLQK
  MSLQVIKENLPENVTASESDAEVERSQDDQHMLPFWTDLCSIFGSNKDLMGLAINDSFLSASLVRILCEQ
  IASDTCHLQRVVFKNISPADAHRNLCLALRGHKTVTYLTLQGNDQDDMFPALCEVLRHPECNLRYLGLVS
  CSATTQQWADLSLALEVNQSLTCVNLSDNELLDEGAKLLYTTLRHPKCFLQRLSLENCHLTEANCKDLAA
  VLVVSRELTHLCLAKNPIGNTGVKFLCEGLRYPECKLQTLVLWNCDITSDGCCDLTKLLQEKSSLLCLDL
  GLNHIGVKGMKFLCEALRKPLCNLRCLWLWGCSIPPFSCEDLCSALSCNQSLVTLDLGQNPLGSSGVKML
  FETLTCSSGTLRTLRLKIDDFNDELNKLLEEIEEKNPQLIIDTEKHHPWAERPSSHDFMI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174082
• ORF Size: 3120 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001174082.1, NP_001167553.1
• RefSeq Size: 3524
• RefSeq ORF: 3123
• Locus ID: 55655
• Gene Summary: This gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). Members of this gene family are thought to be important regulators of immune responses. This gene product interacts with components of the IkB kinase (IKK) complex, and can regulate both caspase-1 and NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity. The pyrin domain is necessary and sufficient for suppression of NF-kB activity. An allelic variant (rs147585490) has been found that is incapable of blocking the transcriptional activity of NF-kB. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]