NCKAP1L (NM_001184976) Human Tagged ORF Clone
CAT#: RG230643
- TrueORF®
NCKAP1L (GFP-tagged) - Human NCK-associated protein 1-like (NCKAP1L), transcript variant 2
"NM_001184976" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | NCKAP1L |
Synonyms | HEM1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG230643 representing NM_001184976
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCATCTCTCAAGTATATCAACAAGAAATTTCCCAACATAGATGTCCGAAACAGCACGCAACATT TAGGACCAGTACATCGTGAAAAAGCCGAGATAATTAGATTCCTCACCAACTACTACCAGTCATTTGTGGA TGTCATGGAATTTCGGGATCATGTATATGAACTTCTCAACACCATTGATGCCTGCCAGTGCCATTTTGAT ATCAATCTCAACTTTGATTTCACTCGGAGTTACCTGGACTTGATTGTAACTTACACCTCAGTCATTTTAC TTCTGTCACGGATTGAAGATCGGCGGATACTCATTGGCATGTACAATTGTGCCCATGAGATGCTGCATGG GCATGGTGACCCCAGTTTTGCCCGTCTGGGTCAGATGGTCTTGGAGTATGACCACCCTCTGAAGAAGCTG ACAGAAGAGTTTGGGCCTCACACAAAGGCTGTGAGTGGAGCCCTCCTCTCTTTGCATTTCCTCTTTGTCC GAAGAAACCAGGGGGCTGAGCAGTGGCGCAGTGCCCAACTTCTAAGCCTCATCAGCAACCCCCCAGCCAT GATTAACCCTGCTAATTCAGATACAATGGCCTGTGAGTATCTGTCTGTGGAAGTAATGGAGCGCTGGATT ATCATTGGGTTTCTTCTTTGTCATGGGTGCCTCAACTCCAATAGCCAGTGCCAGAAGCTGTGGAAGCTGT GTCTGCAGGGCTCCCTCTACATCACCCTTATCCGTGAGGATGTGCTGCAGGTGCACAAAGTCACCGAGGA CCTGTTTAGCAGTTTGAAAGGGTATGGCAAGAGAGTGGCAGACATAAAGGAGAGCAAGGAACATGTAATT GCAAACAGTGGCCAGTTTCATTGTCAACGGCGGCAATTTCTGCGGATGGCAGTGAAGGAGCTGGAGACTG TGTTGGCTGATGAACCGGGACTACTGGGTCCTAAGGCTCTTTTTGCTTTCATGGCCCTGTCCTTCATTCG TGATGAGGTCACCTGGCTGGTTCGCCACACAGAGAATGTCACCAAGACAAAGACACCTGAGGACTATGCT GACTCGAGCATTGCAGAGCTACTTTTCTTGTTGGAGGGGATTAGGTCTCTGGTCCGAAGACACATCAAAG TGATACAGCAATACCACCTTCAGTACTTGGCAAGATTTGATGCTCTTGTGCTCAGTGACATCATTCAGAA CTTGTCTGTGTGTCCAGAGGAGGAGTCCATCATCATGTCCTCATTCGTCAGTATCCTCTCCTCTCTGAAT CTCAAACAAGTTGATAATGGAGAAAAATTTGAATTCTCAGGATTGAGGCTGGACTGGTTCCGCCTACAGG CATACACTAGCGTGGCTAAGGCCCCTCTGCACCTGCATGAGAACCCTGACTTAGCCAAGGTGATGAACCT CATTGTCTTCCACTCCCGAATGCTGGACTCCGTAGAAAAATTGCTGGTGGAAACTTCTGATCTGTCTACT TTCTGCTTTCATCTTCGTATCTTTGAGAAGATGTTTGCCATGACCTTGGAGGAATCTGCCATGTTGCGTT ATGCCATTGCTTTCCCCCTGATTTGTGCTCACTTTGTCCACTGCACTCATGAGATGTGCCCAGAGGAGTA CCCCCACCTCAAGAACCATGGTCTTCACCACTGCAACTCCTTCCTGGAAGAGTTGGCCAAGCAGACCAGC AATTGCGTCCTGGAGATCTGTGCTGAGCAGCGAAACCTGAGCGAGCAGCTTCTACCTAAGCACTGTGCCA CTACAATCAGCAAAGCCAAGAACAAGAAAACCAGGAAGCAGAGGCAGACTCCCAGAAAAGGAGAGCCCGA GAGGGACAAGCCAGGAGCTGAGAGTCACCGGAAGAACCGCAGCATTGTCACCAACATGGACAAGCTACAC CTAAACTTGACAGAACTGGCACTGACAATGAATCATGTATACAGTTTCTCCGTGTTTGAACATACTATCT TCCCTTCTGAGTACCTCAGCAGCCACCTGGAGGCCAGACTCAACAGAGCCATTGTGTGGCTGGCTGGCTA CAATGCCACGACCCAGGAGATCGTACGGCCTTCTGAGCTGTTGGCAGGAGTCAAAGCATACATTGGTTTC ATACAGTCACTGGCCCAGTTTTTGGGTGCAGATGCTTCCAGAGTCATCCGCAACGCCCTCCTGCAGCAGA CACAACCACTGGATTCCTGTGGGGAACAGACAATCACCACACTCTACACAAACTGGTACCTGGAAAGTCT GCTTAGACAGGCAAGCAGTGGGACCATCATCCTCTCCCCAGCCATGCAGGCCTTCGTCAGCCTGCCCAGA GAAGGGGAGCAGAACTTCAGTGCAGAGGAGTTCTCTGACATCTCTGAGATGCGGGCCTTGGCAGAACTCC TGGGCCCCTATGGCATGAAGTTCCTGAGTGAAAACCTGATGTGGCATGTGACCTCTCAGATTGTGGAGCT GAAGAAGCTGGTGGTGGAAAACATGGACATACTTGTTCAGATCAGATCCAACTTTAGCAAGCCGGACTTG ATGGCTTCCCTGCTGCCCCAGCTGACAGGGGCTGAAAATGTGCTAAAGCGCATGACCATCATTGGGGTTA TCCTCAGTTTCAGGGCCATGGCCCAAGAGGGACTTCGGGAGGTTTTCTCCTCCCACTGCCCATTTCTTAT GGGTCCCATTGAGTGCTTGAAGGAGTTTGTCACTCCAGACACAGACATCAAGGTGACCTTGAGTATCTTT GAGCTGGCATCTGCTGCAGGTGTGGGCTGTGACATTGACCCAGCCTTGGTGGCTGCCATTGCTAATCTGA AAGCTGATACTTCATCTCCTGAGGAGGAATATAAGGTGGCCTGCCTGCTCTTGATCTTTCTGGCAGTTTC CCTCCCACTCCTTGCCACTGACCCTTCTTCCTTTTATAGCATTGAGAAGGATGGTTACAACAACAATATT CATTGCTTGACCAAAGCCATCATCCAGGTGTCTGCTGCCCTCTTCACGCTCTACAACAAGAACATTGAAA CTCACCTCAAGGAATTTCTGGTGGTGGCCTCTGTCAGCCTCTTGCAGCTGGGCCAGGAGACTGACAAGCT TAAAACCAGAAATCGAGAATCCATTTCTCTGCTCATGCGCTTGGTGGTGGAGGAGTCATCCTTCCTGACC CTGGACATGCTGGAGTCCTGTTTCCCTTATGTCCTGCTTCGAAATGCCTATCGGGAGGTGTCTCGGGCCT TCCACCTAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230643 representing NM_001184976
Red=Cloning site Green=Tags(s) MEPSLKYINKKFPNIDVRNSTQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFD INLNFDFTRSYLDLIVTYTSVILLLSRIEDRRILIGMYNCAHEMLHGHGDPSFARLGQMVLEYDHPLKKL TEEFGPHTKAVSGALLSLHFLFVRRNQGAEQWRSAQLLSLISNPPAMINPANSDTMACEYLSVEVMERWI IIGFLLCHGCLNSNSQCQKLWKLCLQGSLYITLIREDVLQVHKVTEDLFSSLKGYGKRVADIKESKEHVI ANSGQFHCQRRQFLRMAVKELETVLADEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYA DSSIAELLFLLEGIRSLVRRHIKVIQQYHLQYLARFDALVLSDIIQNLSVCPEEESIIMSSFVSILSSLN LKQVDNGEKFEFSGLRLDWFRLQAYTSVAKAPLHLHENPDLAKVMNLIVFHSRMLDSVEKLLVETSDLST FCFHLRIFEKMFAMTLEESAMLRYAIAFPLICAHFVHCTHEMCPEEYPHLKNHGLHHCNSFLEELAKQTS NCVLEICAEQRNLSEQLLPKHCATTISKAKNKKTRKQRQTPRKGEPERDKPGAESHRKNRSIVTNMDKLH LNLTELALTMNHVYSFSVFEHTIFPSEYLSSHLEARLNRAIVWLAGYNATTQEIVRPSELLAGVKAYIGF IQSLAQFLGADASRVIRNALLQQTQPLDSCGEQTITTLYTNWYLESLLRQASSGTIILSPAMQAFVSLPR EGEQNFSAEEFSDISEMRALAELLGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPDL MASLLPQLTGAENVLKRMTIIGVILSFRAMAQEGLREVFSSHCPFLMGPIECLKEFVTPDTDIKVTLSIF ELASAAGVGCDIDPALVAAIANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNI HCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESSFLT LDMLESCFPYVLLRNAYREVSRAFHLN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001184976 |
ORF Size | 3231 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001184976.1, NP_001171905.1 |
RefSeq Size | 3780 bp |
RefSeq ORF | 3234 bp |
Locus ID | 3071 |
Cytogenetics | 12q13.13-q13.2 |
Protein Pathways | Regulation of actin cytoskeleton |
Gene Summary | 'This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC329281 | NCKAP1L (untagged)-Human NCK-associated protein 1-like (NCKAP1L) transcript variant 2 |
USD 1,100.00 |
|
RC230643 | NCKAP1L (Myc-DDK-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 2 |
USD 1,130.00 |
|
RC230643L3 | Lenti-ORF clone of NCKAP1L (Myc-DDK-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 2 |
USD 1,330.00 |
|
RC230643L4 | Lenti-ORF clone of NCKAP1L (mGFP-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 2 |
USD 1,330.00 |
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