SLC4A10 (NM_001178015) Human Tagged ORF Clone

CAT#: RG230654

SLC4A10 (GFP-tagged) - Human solute carrier family 4, sodium bicarbonate transporter, member 10 (SLC4A10), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC4A10
• Synonyms: NBCn2; NCBE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230654 representing NM_001178015
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATTAAAGACCAGGGAGCCCAAATGGAGCCGCTGCTGCCTACGAGAAATGATGAAGAAGCAGTTG
  TGGATAGAGGTGGAACTCGTTCTATTCTCAAAACACACTTTGAGAAAGAAGATTTAGAAGGTCATCGAAC
  ACTATTTATTGGAGTACATGTGCCCTTGGGAGGAAGAAAAAGCCATCGACGTCACAGGCATCGTGGTCAT
  AAACACAGAAAGAGAGACAGAGAAAGAGATTCAGGATTAGAGGATGGAAGGGAGTCACCTTCTTTTGACA
  CCCCATCACAGAGGGTACAGTTTATTCTTGGAACCGAGGATGATGACGAGGAACACATTCCTCATGACCT
  TTTCACAGAACTGGATGAGATTTGTTGGCGTGAAGGTGAGGACGCTGAGTGGCGAGAAACAGCCAGGTGG
  TTGAAGTTTGAAGAAGATGTGGAAGATGGAGGAGAAAGGTGGAGCAAGCCTTATGTGGCTACTCTTTCAT
  TGCACAGCTTGTTTGAATTGAGAAGTTGTATTCTGAATGGAACTGTGTTGCTGGACATGCATGCCAACAC
  TTTAGAAGAAATTGCAGATATGGTTCTTGACCAACAAGTGAGCTCAGGTCAGCTGAATGAAGATGTACGC
  CATAGGGTCCATGAGGCATTGATGAAACAGCATCATCATCAGAATCAGAAAAAACTCACCAACAGGATTC
  CCATTGTTCGTTCCTTTGCTGATATTGGCAAGAAACAGTCAGAACCAAATTCCATGGACAAAAATGCAGG
  TCAGGTTGTTTCTCCTCAGTCTGCTCCAGCCTGTGTTGAAAATAAAAATGATGTTAGCAGAGAAAACAGC
  ACTGTTGACTTTAGCAAGGGACTGGGAGGCCAACAAAAGGGGCATACTAGTCCATGTGGGATGAAACAAA
  GGCATGAAAAAGGACCTCCACACCAGCAAGAGAGAGAGGTTGATCTGCATTTTATGAAAAAGATTCCTCC
  AGGTGCTGAAGCATCGAACATCTTAGTGGGAGAACTGGAGTTCTTGGATCGAACAGTAGTTGCGTTTGTC
  AGGTTGTCTCCAGCTGTATTGCTTCAAGGACTGGCTGAAGTCCCAATCCCAACCAGATTTTTGTTCATTC
  TTCTGGGACCCCTGGGAAAGGGTCAACAGTACCATGAGATTGGCAGATCAATTGCAACCCTAATGACAGA
  TGAGGTATTTCATGATGTTGCCTATAAAGCTAAAGATCGTAATGACTTGGTATCAGGAATTGATGAGTTT
  CTGGATCAGGTTACTGTTCTCCCTCCTGGAGAATGGGATCCAAGCATTCGAATAGAGCCTCCCAAAAATG
  TTCCTTCCCAGGAGAAGAGGAAGATTCCTGCTGTACCAAATGGAACAGCAGCTCATGGGGAAGCAGAGCC
  CCACGGAGGACATAGTGGACCTGAACTCCAGCGAACTGGAAGGATTTTTGGGGGACTTATTTTAGATATC
  AAAAGAAAAGCTCCATACTTCTGGAGTGACTTCAGAGATGCTTTCAGCCTGCAGTGCTTAGCATCTTTTC
  TATTTCTCTACTGCGCGTGTATGTCTCCTGTCATCACGTTTGGAGGACTGCTGGGAGAAGCAACTGAAGG
  GCGTATAAGTGCAATTGAATCTCTCTTTGGAGCATCCATGACCGGGATAGCCTATTCTCTCTTTGGTGGA
  CAGCCTCTTACCATATTAGGCAGTACAGGACCAGTTTTGGTGTTTGAAAAGATTTTGTTTAAATTTTGCA
  AAGAATATGGGCTGTCATACCTATCTTTAAGAGCTAGCATTGGACTTTGGACTGCAACTCTATGTATCAT
  ACTTGTGGCCACAGATGCTAGTTCCCTTGTCTGCTACATCACTCGGTTTACTGAAGAAGCTTTTGCTTCC
  CTGATTTGCATCATTTTCATTTATGAGGCCCTGGAGAAGTTGTTTGAACTCAGTGAAGCATATCCAATCA
  ACATGCATAATGATCTGGAACTGCTGACACAATACTCGTGTAACTGTGTGGAACCGCATAATCCCAGCAA
  TGGCACATTGAAGGAATGGAGGGAATCCAATATTTCTGCCTCTGACATAATTTGGGAGAACCTAACTGTG
  TCAGAATGCAAATCATTGCATGGAGAGTATGTTGGACGGGCCTGTGGCCATGATCACCCATATGTTCCAG
  ATGTTCTATTTTGGTCTGTGATCCTGTTCTTTTCCACAGTTACTCTGTCAGCCACCCTGAAGCAGTTCAA
  GACTAGCAGATATTTTCCAACCAAGGTTCGATCCATAGTGAGTGACTTTGCTGTCTTTCTTACAATTCTG
  TGTATGGTTTTAATTGACTATGCCATTGGGATCCCATCTCCAAAACTACAAGTACCAAGTGTTTTCAAGC
  CCACTAGAGATGATCGTGGCTGGTTTGTTACGCCTTTAGGTCCAAACCCATGGTGGACAGTAATAGCTGC
  TATAATTCCAGCTCTGCTTTGTACTATTCTAATTTTTATGGACCAACAGATTACAGCTGTCATCATCAAC
  AGGAAAGAGCATAAGCTAAAGAAAGGTTGTGGGTACCATCTGGACCTATTAATGGTGGCTGTCATGCTCG
  GTGTATGCTCCATCATGGGCCTGCCATGGTTTGTGGCTGCCACAGTCCTCTCCATCACTCATGTCAATAG
  CCTAAAACTGGAATCAGAATGCTCAGCTCCAGGAGAACAACCCAAATTTCTCGGCATTCGGGAGCAAAGG
  GTTACTGGGCTTATGATTTTTATTCTTATGGGTTCATCAGTCTTTATGACCAGTATTCTGAAGTTTATTC
  CCATGCCAGTGCTATATGGAGTGTTTCTTTATATGGGTGCTTCATCTCTAAAGGGAATTCAGTTCTTTGA
  TAGGATAAAGCTCTTCTGGATGCCGGCAAAACATCAACCAGATTTTATATACCTAAGGCACGTACCGCTT
  CGAAAAGTGCATCTCTTCACAATTATTCAGATGAGTTGCCTTGGCCTTTTGTGGATAATAAAAGTTTCAA
  GAGCTGCTATTGTCTTTCCCATGATGGTGTTAGCCCTGGTATTTGTAAGAAAGTTGATGGACTTGTTGTT
  CACGAAGCGGGAACTCAGCTGGTTGGATGATTTGATGCCCGAGAGTAAGAAAAAGAAACTGGAAGATGCT
  GAAAAAGAAGAAGAACAAAGTATGCTAGCTATGGAAGATGAGGGCACAGTACAACTCCCATTGGAAGGGC
  ACTATAGAGATGATCCATCTGTGATCAATATATCTGATGAAATGTCAAAGACTGCCTTGTGGAGGAACCT
  TCTGATTACTGCCGATAACTCAAAAGATAAGGAGTCAAGCTTTCCTTCCAAAAGCTCCCCTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230654 representing NM_001178015
  Red=Cloning site Green=Tags(s)
  
  MEIKDQGAQMEPLLPTRNDEEAVVDRGGTRSILKTHFEKEDLEGHRTLFIGVHVPLGGRKSHRRHRHRGH
  KHRKRDRERDSGLEDGRESPSFDTPSQRVQFILGTEDDDEEHIPHDLFTELDEICWREGEDAEWRETARW
  LKFEEDVEDGGERWSKPYVATLSLHSLFELRSCILNGTVLLDMHANTLEEIADMVLDQQVSSGQLNEDVR
  HRVHEALMKQHHHQNQKKLTNRIPIVRSFADIGKKQSEPNSMDKNAGQVVSPQSAPACVENKNDVSRENS
  TVDFSKGLGGQQKGHTSPCGMKQRHEKGPPHQQEREVDLHFMKKIPPGAEASNILVGELEFLDRTVVAFV
  RLSPAVLLQGLAEVPIPTRFLFILLGPLGKGQQYHEIGRSIATLMTDEVFHDVAYKAKDRNDLVSGIDEF
  LDQVTVLPPGEWDPSIRIEPPKNVPSQEKRKIPAVPNGTAAHGEAEPHGGHSGPELQRTGRIFGGLILDI
  KRKAPYFWSDFRDAFSLQCLASFLFLYCACMSPVITFGGLLGEATEGRISAIESLFGASMTGIAYSLFGG
  QPLTILGSTGPVLVFEKILFKFCKEYGLSYLSLRASIGLWTATLCIILVATDASSLVCYITRFTEEAFAS
  LICIIFIYEALEKLFELSEAYPINMHNDLELLTQYSCNCVEPHNPSNGTLKEWRESNISASDIIWENLTV
  SECKSLHGEYVGRACGHDHPYVPDVLFWSVILFFSTVTLSATLKQFKTSRYFPTKVRSIVSDFAVFLTIL
  CMVLIDYAIGIPSPKLQVPSVFKPTRDDRGWFVTPLGPNPWWTVIAAIIPALLCTILIFMDQQITAVIIN
  RKEHKLKKGCGYHLDLLMVAVMLGVCSIMGLPWFVAATVLSITHVNSLKLESECSAPGEQPKFLGIREQR
  VTGLMIFILMGSSVFMTSILKFIPMPVLYGVFLYMGASSLKGIQFFDRIKLFWMPAKHQPDFIYLRHVPL
  RKVHLFTIIQMSCLGLLWIIKVSRAAIVFPMMVLALVFVRKLMDLLFTKRELSWLDDLMPESKKKKLEDA
  EKEEEQSMLAMEDEGTVQLPLEGHYRDDPSVINISDEMSKTALWRNLLITADNSKDKESSFPSKSSPS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178015
• ORF Size: 3354 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001178015.1, NP_001171486.1
• RefSeq Size: 5671
• RefSeq ORF: 3357
• Locus ID: 57282
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene belongs to a small family of sodium-coupled bicarbonate transporters (NCBTs) that regulate the intracellular pH of neurons, the secretion of bicarbonate ions across the choroid plexus, and the pH of the brain extracellular fluid. The protein encoded by this gene was initially identified as a sodium-driven chloride bicarbonate exchanger (NCBE) though there is now evidence that its sodium/bicarbonate cotransport activity is independent of any chloride ion countertransport under physiological conditions. This gene is now classified as a member A10 of the SLC4 family of transmembrane solute carriers. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2010]