RGNEF (ARHGEF28) (NM_001177693) Human Tagged ORF Clone

CAT#: RG230731

ARHGEF28 (GFP-tagged) - Human 190 kDa guanine nucleotide exchange factor (RGNEF), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARHGEF28
• Synonyms: p190RHOGEF; RGNEF; RIP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230731 representing NM_001177693
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGAGCTGCAGCGAAGCACCTCTTTACGGGCAGATGATGATCTATGCGAAGTTTGACAAAAATG
  TGTATCTTCCTGAAGATGCTGAGTTTTACTTTACTTATGACGGATCTCATCAGCGACATGTCATGATTGC
  AGAGCGCATCGAGGATAACGTTCTCCAGTCCAGCGTCCCAGGCCATGGGCTTCAGGAGACGGTGACGGTA
  TCTGTGTGCCTCTGCTCGGAAGGTTACTCTCCGGTGACCATGGGCTCTGGCTCAGTGACCTACGTGGACA
  ACATGGCTTGCAGGCTGGCTCGTCTGCTGGTGACGCAGGCCAATCGCCTCACAGCCTGCAGCCACCAGAC
  CCTGCTGACCCCATTTGCCTTGACGGCAGGAGCACTGCCTGCCTTGGATGAGGAGCTCGTGCTGGCTCTG
  ACCCATCTGGAATTGCCTCTAGAGTGGACTGTGTTGGGAAGTTCTTCACTTGAAGTATCTTCTCACAGAG
  AATCTCTTCTACACCTGGCTATGAGATGGGGCCTGGCTAAACTTTCCCAGTTCTTCTTGTGTCTCCCGGG
  GGGAGTCCAGGCCTTGGCTTTACCCAACGAAGAGGGTGCCACACCATTAGACTTAGCTTTACGTGAAGGA
  CACTCCAAGCTGGTGGAAGACGTCACAAATTTTCAGGGCAGATGGTCCCCAAGCTTCTCCCGAGTGCAGC
  TCAGTGAAGAAGCCTCCTTGCATTACATTCACTCATCGGAAACGCTGACCCTGACCCTGAACCACACAGC
  CGAGCATTTGTTGGAGGCAGATATTAAACTCTTCCGGAAATACTTTTGGGATAGAGCCTTTCTTGTCAAG
  GCCTTTGAGCCAGAAGCCAGGCCAGAGGAAAGAACAGCTATGCCCTCCAGCGGTGCAGAAACTGAAGAAG
  AGATTAAGAATTCAGTGTCCAGCAGATCAGCAGCTGAAAAGGAAGATATAAAGCGTGTCAAAAGCCTGGT
  GGTTCAACACAATGAACATGAAGACCAGCACAGCCTAGATTTGGATCGCTCCTTCGATATCCTAAAAAAA
  TCCAAGCCGCCCTCGACATTGCTTGCTGCAGGCCGGCTTTCAGACATGCTGAATGGAGGTGATGAAGTCT
  ACGCTAACTGTATGGTGATTGATCAGGTTGGTGATTTGGATATCAGCTATATTAATATAGAGGGAATCAC
  TGCCACTACCAGCCCTGAATCCAGAGGTTGCACTCTGTGGCCTCAGAGCAGCAAACACACCCTTCCTACA
  GAAACCAGTCCCAGTGTGTACCCACTTAGTGAAAATGTCGAAGGGACAGCACACACTGAAGCCCAGCAGT
  CCTTCATGTCACCATCAAGTTCGTGTGCTTCCAACTTGAATCTTTCTTTTGGTTGGCATGGATTTGAAAA
  GGAACAAAGTCATCTAAAGAAAAGAAGTTCTAGCCTTGATGCCTTGGACGCCGACAGTGAAGGGGAAGGG
  CATTCTGAGCCATCCCACATCTGTTACACTCCAGGGTCTCAGAGCTCCTCAAGAACTGGGATTCCTAGTG
  GGGATGAATTGGACTCTTTTGAGACTAACACTGAACCGGATTTTAATATCTCCAGGGCTGAATCCCTTCC
  TCTATCAAGTAATCTACAGTCGAAGGAATCACTGCTTTCTGGAGTTCGCTCACGTTCTTATTCTTGCTCA
  TCACCCAAAATTTCTTTAGGAAAAACTCGTTTGGTGCGTGAATTAACAGTATGCAGTTCAAGTGAAGAGC
  AAAGAGCTTACAGCTTATCGGAGCCACCAAGAGAAAACAGAATTCAGGAAGAAGAATGGGATAAATACAT
  CATACCTGCCAAATCAGAGTCTGAAAAATATAAAGTGAGTCGAACTTTCAGTTTCCTCATGAATAGGATG
  ACTAGCCCTCGGAATAAATCAAAGACAAAAAGCAAGGATGCCAAAGATAAAGAGAAGCTGAATCGACATC
  AGTTTGCCCCAGGAACATTCTCTGGGGTTCTGCAGTGTTTGGTTTGTGATAAAACACTCCTGGGGAAAGA
  GTCACTGCAGTGTTCTAACTGTAATGCAAATGTGCACAAAGGTTGTAAAGATGCTGCGCCTGCATGCACC
  AAGAAATTCCAAGAGAAATATAACAAGAACAAACCACAGACCATCCTTGGAAATTCTTCATTTAGAGACA
  TCCCACAGCCTGGTCTCTCCTTGCACCCTTCTTCCTCCGTGCCTGTTGGATTGCCGACTGGAAGGAGGGA
  GACTGTGGGACAGGTCCATCCATTGTCCAGAAGTGTTCCAGGCACCACCTTGGAAAGCTTCAGGAGGTCA
  GCCACATCCTTGGAGTCTGAGAGTGACCATAACAGCTGCAGAAGCAGGTCTCATTCTGATGAGCTGCTAC
  AGTCCATGGGCTCTTCTCCCTCTACAGAGTCTTTCATAATGGAAGATGTTGTGGATTCTTCTCTGTGGAG
  TGACCTCAGCAGTGATGCCCAGGAGTTTGAAGCAGAATCTTGGAGTCTTGTGGTGGATCCCTCATTTTGT
  AATAGGCAGGAGAAGGATGTCATCAAAAGACAGGATGTCATTTTTGAGCTAATGCAAACAGAGATGCATC
  ACATCCAGACCCTGTTCATCATGTCTGAGATCTTCAGGAAAGGCATGAAAGAGGAGCTGCAGCTGGACCA
  CAGCACCGTGGATAAAATTTTCCCCTGTTTAGATGAGTTGCTTGAAATCCACAGGCATTTCTTCTACAGT
  ATGAAGGAACGAAGGCAGGAATCCTGTGCTGGCAGCGACAGGAATTTTGTGATCGACCGAATTGGAGATA
  TTTTGGTACAACAGTTTTCAGAAGAAAATGCAAGTAAAATGAAGAAAATATATGGAGAATTCTGTTGCCA
  TCATAAAGAAGCTGTTAACCTCTTTAAAGAACTCCAGCAGAATAAAAAGTTTCAGAATTTTATTAAGCTC
  CGAAATAGTAATCTTTTGGCTCGACGCCGAGGAATTCCAGAATGCATTCTGTTGGTCACTCAGCGTATTA
  CAAAATACCCTGTCTTGGTGGAAAGGATATTGCAGTACACAAAGGAAAGAACTGAGGAACATAAAGACTT
  ACGCAAAGCGCTTTGCTTAATTAAAGACATGATTGCAACAGTGGATTTAAAAGTCAATGAATATGAGAAA
  AACCAAAAATGGCTTGAGATCCTAAATAAGATTGAAAACAAAACATACACGAAGCTCAAAAATGGACATG
  TGTTTAGGAAGCAGGCACTGATGAGTGAAGAAAGGACTCTGTTATATGATGGCCTTGTTTACTGGAAAAC
  TGCTACAGGTCGTTTCAAAGATATCCTAGCTCTACTTCTAACTGATGTGCTGCTCTTTTTACAAGAAAAA
  GACCAGAAATACATCTTTGCAGCCGTTGATCAGAAGCCATCAGTTATTTCCCTTCAAAAGCTTATTGCTA
  GAGAAGTTGCTAATGAGGAGAGAGGAATGTTTCTGATCAGTGCTTCATCTGCTGGTCCTGAGATGTATGA
  AATTCACACCAATTCCAAGGAGGAACGCAATAACTGGATGAGACGGATCCAGCAGGCTGTAGAAAGTTGT
  CCTGAAGAAAAAGGGGGAAGGACAAGTGAATCTGATGAAGACAAGAGGAAAGCTGAAGCCAGAGTGGCCA
  AAATTCAGCAATGTCAAGAAATACTCACTAACCAAGACCAACAAATTTGTGCGTATTTGGAGGAGAAGCT
  GCATATCTATGCTGAACTTGGAGAACTGAGCGGATTTGAGGACGTCCATCTAGAGCCCCACCTCCTTATT
  AAACCTGACCCAGGCGAGCCTCCCCAGGCAGCCTCATTACTGGCAGCAGCACTGAAAGAAGCTGAGAGCC
  TACAAGTTGCAGTGAAGGCCTCACAGATGGGCGCCGTGAGTCAATCATGTGAGGACAGTTGTGGAGACTC
  TGTCTTGGCGGACACACTCAGTTCTCATGATGTACCAGGATCACCGACTGCCTCATTAGTCACAGGAGGG
  AGAGAAGGAAGAGGCTGTTCGGATGTGGATCCCGGGATCCAGGGTGTGGTAACCGACTTGGCCGTCTCTG
  ATGCAGGGGAGAAGGTGGAATGTAGAAATTTTCCAGGTTCTTCACAATCAGAGATTATACAAGCCATACA
  GAATTTAACCCGTCTCTTATACAGCCTTCAGGCCGCCTTGACCATTCAGGACAGCCACATTGAGATCCAC
  AGGCTGGTTCTCCAGCAGCAGGAGGGCCTGTCTCTCGGCCACTCTATCCTCCGAGGCGGCCCCTTGCAGG
  ACCAGAAGTCTCGCGACGCGGACAGGCAGCATGAGGAGCTGGCCAATGTGCACCAGCTTCAGCACCAGCT
  CCAGCAGGAGCAGCGGCGCTGGCTGCGCAGGTGTGAGCAGCAGCAGCGGGCGCAGGCGACCAGGGAGAGC
  TGGCTGCAGGAGCGGGAGCGGGAGTGCCAGTCGCAGGAGGAGCTGCTGCTGCGGAGCCGGGGCGAGCTGG
  ACCTCCAGCTCCAGGAGTACCAGCACAGCCTGGAGCGGCTGAGGGAGGGCCAGCGCCTGGTGGAGAGGGA
  GCAGGCGAGGATGCGGGCCCAGCAGAGCCTGCTGGGCCACTGGAAGCACGGCCGGCAGAGGAGCCTGCCC
  GCGGTGCTCCTTCCGGGTGGCCCCGAGGTAATGGAACTTAATCGATCTGAGAGTTTATGTCATGAAAACT
  CATTCTTCATCAATGAAGCTTTAGTACAAATGTCATTTAACACTTTCAACAAACTGAATCCATCAGTTAT
  CCATCAGGATGCCACTTACCCTACAACTCAATCTCATTCTGACTTGGTGAGGACTAGTGAACATCAAGTA
  GACCTCAAGGTGGACCCTTCTCAGCCTTCGAATGTCAGTCACAAACTGTGGACAGCCGCTGGTTCCGGCC
  ATCAGATACTTCCTTTCCATGAAAGCAGCAAGGATTCTTGTAAAAATGATTTGGACACCTCCCACACTGA
  GTCCCCAACCCCCCATGACTCAAATTCACACCGCCCTCAACTGCAGGCGTTTATAACAGAAGCAAAGCTA
  AATCTACCGACAAGGACAATGACCAGACAAGATGGGGAAACTGGAGATGGAGCCAAAGAAAATATTGTTT
  ACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230731 representing NM_001177693
  Red=Cloning site Green=Tags(s)
  
  MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVPGHGLQETVTV
  SVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQANRLTACSHQTLLTPFALTAGALPALDEELVLAL
  THLELPLEWTVLGSSSLEVSSHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREG
  HSKLVEDVTNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVK
  AFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKK
  SKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPT
  ETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEG
  HSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCS
  SPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRM
  TSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACT
  KKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRS
  ATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFC
  NRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYS
  MKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKL
  RNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEK
  NQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEK
  DQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESC
  PEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLI
  KPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGG
  REGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIH
  RLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRES
  WLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLP
  AVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQV
  DLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNDLDTSHTESPTPHDSNSHRPQLQAFITEAKL
  NLPTRTMTRQDGETGDGAKENIVYL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177693
• ORF Size: 5115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001177693.1, NP_001171164.1
• RefSeq Size: 6291
• RefSeq ORF: 5118
• Locus ID: 64283
• Gene Summary: This gene encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]