TRPM6 (NM_001177310) Human Tagged ORF Clone

CAT#: RG230739

TRPM6 (GFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 6 (TRPM6), transcript variant b

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRPM6
• Synonyms: CHAK2; HMGX; HOMG; HOMG1; HSH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230739 representing NM_001177310
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTATCCTATCTAAGTCCCAGAAATCCTGGATTAAAGGAGTATTTGACAAGAGAGAATGTAGCACAA
  TCATACCCAGCTCAAAAAATCCTCACAGATGTACTCCAGTATGCCAAGTCTGCCAGAATTTAATCAGGTG
  TTACTGTGGCCGACTGATTGGAGACCATGCTGGGATAGATTATTCCTGGACCATCTCAGCTGCCAAGGGT
  AAAGAAAGTGAACAATGGTCTGTTGAAAAGCACACAACGAAAAGCCCAACAGATACTTTTGGCACGATTA
  ATTTCCAAGATGGAGAGCACACCCATCATGCCAAGTATATTAGAACTTCTTATGATACAAAACTGGATCA
  TCTGTTACATTTAATGTTGAAAGAGTGGAAAATGGAACTGCCCAAGCTTGTGATCTCAGTCCATGGGGGC
  ATCCAGAACTTTACTATGCCCTCTAAATTTAAAGAGATTTTCAGCCAAGGTTTGGTTAAAGCTGCAGAGA
  CAACAGGAGCGTGGATAATAACTGAAGGCATCAATACAGGAGTGTCCAAGCATGTTGGGGATGCCTTGAA
  ATCCCATTCCTCTCATTCCTTGAGAAAAATCTGGACAGTTGGAATCCCTCCTTGGGGTGTCATTGAGAAC
  CAGAGAGACCTTATTGGAAAAGATGTGGTGTGCCTGTACCAGACTCTGGATAACCCCCTCAGCAAGCTCA
  CAACACTCAACAGCATGCACTCGCACTTCATCCTGTCTGATGATGGGACCGTGGGCAAGTATGGAAATGA
  AATGAAGCTCAGAAGGAACCTGGAGAAGTACCTCTCTCTGCAGAAAATACACTGCCGCTCAAGACAAGGC
  GTGCCGGTCGTGGGGCTGGTGGTGGAAGGCGGTCCCAACGTCATCCTGTCAGTGTGGGAGACTGTCAAGG
  ACAAGGACCCAGTGGTGGTGTGTGAGGGCACAGGTAGGGCGGCTGACCTCCTGGCCTTCACACACAAACA
  CCTGGCAGATGAAGGGATGCTGCGACCTCAGGTGAAAGAGGAGATCATCTGCATGATTCAGAACACTTTC
  AACTTTAGTCTTAAACAGTCCAAGCACCTTTTCCAAATTCTAATGGAGTGTATGGTTCACAGGGATTGTA
  TTACCATATTTGATGCTGACTCTGAAGAGCAGCAAGACCTGGACTTAGCAATCCTAACAGCTTTGCTGAA
  GGGCACAAATTTATCAGCGTCAGAGCAATTAAATCTGGCAATGGCTTGGGACAGGGTGGACATTGCCAAG
  AAACATATCCTAATTTATGAACAACACTGGAAGCCTGATGCCCTGGAACAAGCAATGTCAGATGCTTTAG
  TGATGGATCGGGTGGATTTTGTGAAGCTCTTAATAGAATATGGAGTGAACCTCCATCGCTTTCTTACCAT
  CCCTCGACTGGAAGAGCTCTACAATACAAAACAAGGACCTACTAATACACTCTTGCATCATCTCGTCCAA
  GATGTGAAACAGCATACCCTTCTTTCAGGCTACCGAATAACCTTGATTGACATTGGATTAGTAGTAGAAT
  ACCTCATTGGTAGAGCATATCGCAGCAACTACACTAGAAAACATTTCAGAGCCCTCTACAACAACCTCTA
  CAGAAAATACAAGCACCAGAGACACTCCTCAGGAAATAGAAATGAGTCTGCAGAAAGTACGCTGCACTCC
  CAGTTCATTAGAACTGCACAGCCATACAAATTCAAGGAAAAGTCTATAGTCCTTCATAAATCAAGGAAGA
  AGTCAAAAGAACAAAATGTATCAGATGACCCTGAGTCTACTGGCTTTCTTTACCCTTACAATGACCTGCT
  GGTTTGGGCTGTGCTGATGAAAAGGCAGAAGATGGCTATGTTCTTCTGGCAGCATGGAGAGGAGGCCACG
  GTTAAAGCCGTGATTGCGTGTATCCTCTACCGGGCAATGGCCCATGAAGCTAAGGAGAGTCACATGGTGG
  ATGATGCCTCAGAAGAGTTGAAGAATTACTCAAAACAGTTTGGCCAGCTGGCTCTGGACTTGTTGGAGAA
  GGCATTCAAGCAGAATGAGCGCATGGCCATGACGCTGTTGACGTATGAACTCAGGAACTGGAGCAATTCG
  ACCTGCCTTAAACTGGCCGTGTCGGGAGGATTACGACCCTTTGTTTCACATACTTGTACCCAGATGCTAC
  TGACAGACATGTGGATGGGGAGGCTGAAAATGAGGAAAAACTCTTGGTTAAAGATTATTATAAGCATTAT
  TTTACCACCCACCATTTTGACACTGGAATTTAAAAGCAAAGCTGAGATGTCACATGTTCCCCAGTCCCAG
  GACTTCCAATTTATGTGGTATTACAGTGACCAGAACGCCAGCAGTTCCAAAGAAAGTGCTTCTGTGAAAG
  AGTATGATTTGGAAAGGGGCCATGATGAGAAACTGGATGAAAATCAGCATTTTGGTTTGGAAAGTGGGCA
  CCAACACCTTCCGTGGACCAGGAAAGTCTATGAGTTCTACAGTGCTCCAATTGTCAAGTTTTGGTTTTAT
  ACGATGGCGTATTTGGCATTCCTCATGCTGTTCACTTACACCGTGTTGGTGGAGATGCAGCCCCAGCCCA
  GCGTGCAGGAGTGGCTTGTTAGCATTTACATCTTCACCAATGCTATTGAGGTGGTCAGGGAGATCTGTAT
  TTCAGAACCTGGGAAGTTTACCCAAAAGGTGAAGGTATGGATTAGTGAGTACTGGAACTTAACAGAAACT
  GTGGCCATTGGCCTGTTTTCAGCTGGCTTCGTCCTTCGATGGGGTGACCCTCCTTTTCACACAGCGGGAA
  GACTGATCTACTGCATAGACATCATATTCTGGTTCTCACGGCTCCTGGACTTCTTTGCTGTGAATCAACA
  TGCAGGTCCATATGTGACCATGATTGCAAAAATGACAGCAAACATGTTCTATATTGTGATCATCATGGCC
  ATAGTCCTGCTGAGCTTTGGAGTGGCACGCAAGGCCATCCTTTCGCCAAAAGAGCCACCATCTTGGAGTC
  TAGCTCGAGATATTGTATTTGAGCCATACTGGATGATATACGGAGAAGTCTATGCTGGAGAAATAGATGT
  TTGTTCAAGCCAGCCATCCTGCCCTCCTGGTTCTTTTCTTACTCCATTCTTGCAAGCTGTCTACCTCTTC
  GTGCAATATATCATCATGGTGAACCTGTTGATTGCTTTCTTCAACAACGTTTACTTAGATATGGAATCCA
  TTTCAAATAACCTGTGGAAATACAACCGCTATCGCTACATCATGACCTACCACGAGAAGCCCTGGCTGCC
  CCCACCTCTCATCCTGCTGAGCCACGTGGGCCTTCTCCTCCGCCGCCTGTGCTGTCATCGAGCTCCTCAC
  GACCAAGAAGAGGGTGACGTTGGATTAAAACTCTACCTCAGTAAGGAGGATCTGAAAAAACTTCATGATT
  TTGAGGAGCAGTGCGTGGAAAAATACTTCCATGAGAAGATGGAAGATGTGAATTGTAGTTGTGAGGAACG
  AATCCGAGTGACATCAGAAAGGGTTACAGAGATGTACTTCCAGCTGAAAGAAATGAATGAAAAGGTGTCT
  TTTATAAAGGACTCCTTACTGTCTTTGGACAGCCAGGTGGGACACCTGCAGGATCTCTCTGCCCTGACTG
  TGGATACCCTGAAAGTCCTTTCTGCTGTTGACACTTTGCAAGAGGATGAGGCTCTCCTGGCCAAGAGAAA
  GCATTCTACTTGCAAAAAACTTCCCCACAGCTGGAGCAATGTCATCTGTGCAGAGGTTCTAGGCAGCATG
  GAGATCGCTGGAGAGAAGAAATACCAGTATTATAGCATGCCCTCTTCTTTGCTGAGGAGCCTGGCTGGAG
  GCCGGCATCCCCCAAGAGTGCAGAGGGGGGCACTTCTTGAGATTACAAACAGTAAAAGAGAGGCTACAAA
  TGTAAGAAATGACCAGGAAAGGCAAGAAACACAAAGTAGTATAGTGGTTTCTGGGGTGTCTCCTAACAGG
  CAAGCACACTCAAAGTATGGCCAGTTTCTTCTGGTCCCCTCTAATCTAAAGCGAGTTCCTTTTTCAGCAG
  AAACTGTCTTGCCTCTGTCCAGACCCTCTGTGCCAGATGTGCTGGCAACTGAACAGGACATCCAGACTGA
  GGTTCTTGTTCATCTGACTGGGCAGACCCCAGTTGTCTCTGACTGGGCATCAGTGGATGAACCCAAGGAA
  AAGCACGAGCCTATTGCTCACTTACTGGATGGACAAGACAAGGCAGAGCAAGTGCTACCCACTTTGAGTT
  GCACACCTGAACCCATGACAATGAGCTCCCCTCTTTCCCAAGCCAAGATCATGCAAACTGGAGGTGGATA
  TGTAAACTGGGCATTTTCAGAAGGTGATGAAACTGGTGTGTTTAGCATCAAGAAAAAGTGGCAAACCTGC
  TTGCCCTCCACTTGTGACAGTGATTCCTCTCGGAGTGAACAGCACCAGAAGCAGGCCCAGGACAGCTCCC
  TATCTGATAACTCAACAAGATCGGCCCAGAGTAGTGAATGCTCAGAGGTGGGACCATGGCTTCAGCCAAA
  CACATCCTTTTGGATCAATCCTCTCCGCAGATACAGGCCCTTCGCTAGGAGTCATAGTTTTAGATTCCAT
  AAGGAGGAGAAATTGATGAAGATCTGTAAGATTAAAAATCTTTCAGGCTCTTCAGAAATAGGGCAGGGAG
  CATGGGTCAAAGCGAAAATGCTAACCAAAGACAGGAGACTGTCAAAGAAAAAGAAGAATACTCAAGGACT
  CCAGGTGCCAATCATAACAGTCAATGCCTGCTCTCAGAGTGACCAGTTGAATCCAGAGCCAGGAGAAAAC
  AGCATCTCTGAAGAGGAGTACAGCAAGAACTGGTTCACAGTGTCCAAATTTAGTCACACAGGTGTAGAAC
  CTTACATACATCAGAAAATGAAAACTAAAGAAATTGGACAATGTGCTATACAAATCAGTGATTACCTAAA
  GCAGTCTCAAGAGGATCTCAGCAAAAACTCTTTGTGGAATTCCAGGAGCACCAACCTCAATAGGAACTCC
  CTGCTGAAAAGTTCAATTGGAGTTGACAAGATCTCAGCCTCCTTAAAAAGCCCTCAAGAGCCTCACCATC
  ATTATTCAGCCATTGAAAGGAATAATTTAATGAGGCTTTCTCAGACCATACCATTTACACCAGTCCAACT
  GTTTGCAGGAGAAGAAATAACTGTCTACAGGTTGGAGGAGAGTTCCCCTTTAAACCTTGATAAAAGCATG
  TCCTCTTGGTCTCAGCGTGGGAGAGCGGCAATGATCCAGGTATTGTCCCGAGAGGAGATGGATGGGGGCC
  TCCGTAAAGCTATGAGAGTCGTCAGCACTTGGTCTGAGGATGACATTCTCAAGCCGGGACAAGTTTTCAT
  TGTCAAGTCCTTTCTTCCTGAGGTTGTGCGGACATGGCATAAAATCTTCCAGGAGAGCACTGTGCTTCAT
  CTTTGCCTCAGGGAAATTCAACAACAAAGAGCTGCTCAAAAATTGATCTATACCTTCAACCAAGTGAAAC
  CACAAACCATACCCTACACACCAAGGTTCCTGGAAGTTTTCTTAATCTACTGCCATTCAGCCAACCAGTG
  GTTGACCATTGAGAAGTATATGACAGGGGAGTTCCGGAAGTATAACAACAACAATGGTGATGAAATCACC
  CCCACCAACACCCTGGAGGAGCTGATGTTGGCTTTCTCTCACTGGACCTATGAGTACACTCGGGGAGAGC
  TGCTGGTTTTAGATTTGCAAGGTGTTGGAGAAAATTTGACAGATCCATCTGTTATAAAACCTGAAGTCAA
  ACAATCAAGAGGAATGGTGTTTGGACCGGCCAATTTGGGGGAAGATGCAATTAGAAACTTCATTGCAAAA
  CATCATTGTAACTCCTGCTGCCGGAAGCTCAAACTCCCGGATTTAAAAAGAAATGACTATTCCCCTGAAA
  GGATAAATTCCACCTTTGGACTTGAGATAAAAATAGAATCAGCTGAGGAGCCTCCAGCAAGGGAGACGGG
  TAGAAATTCCCCAGAAGATGATATGCAACTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230739 representing NM_001177310
  Red=Cloning site Green=Tags(s)
  
  MIILSKSQKSWIKGVFDKRECSTIIPSSKNPHRCTPVCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKG
  KESEQWSVEKHTTKSPTDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVISVHGG
  IQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDALKSHSSHSLRKIWTVGIPPWGVIEN
  QRDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQG
  VPVVGLVVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTF
  NFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAMAWDRVDIAK
  KHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLHHLVQ
  DVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHS
  QFIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEAT
  VKAVIACILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNS
  TCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQ
  DFQFMWYYSDQNASSSKESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVKFWFY
  TMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREICISEPGKFTQKVKVWISEYWNLTET
  VAIGLFSAGFVLRWGDPPFHTAGRLIYCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMA
  IVLLSFGVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPPGSFLTPFLQAVYLF
  VQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRYIMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPH
  DQEEGDVGLKLYLSKEDLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMNEKVS
  FIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKRKHSTCKKLPHSWSNVICAEVLGSM
  EIAGEKKYQYYSMPSSLLRSLAGGRHPPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNR
  QAHSKYGQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQTPVVSDWASVDEPKE
  KHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLSQAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTC
  LPSTCDSDSSRSEQHQKQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHSFRFH
  KEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQGLQVPIITVNACSQSDQLNPEPGEN
  SISEEEYSKNWFTVSKFSHTGVEPYIHQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNS
  LLKSSIGVDKISASLKSPQEPHHHYSAIERNNLMRLSQTIPFTPVQLFAGEEITVYRLEESSPLNLDKSM
  SSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSEDDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLH
  LCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNGDEIT
  PTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEVKQSRGMVFGPANLGEDAIRNFIAK
  HHCNSCCRKLKLPDLKRNDYSPERINSTFGLEIKIESAEEPPARETGRNSPEDDMQL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177310
• ORF Size: 6051 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001177310.1, NP_001170781.1
• RefSeq Size: 8250
• RefSeq ORF: 6054
• Locus ID: 140803
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Protein Kinase, Transmembrane
• Gene Summary: This gene is predominantly expressed in the kidney and colon, and encodes a protein containing an ion channel domain and a protein kinase domain. It is crucial for magnesium homeostasis, and plays an essential role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. Mutations in this gene are associated with hypomagnesemia with secondary hypocalcemia. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Apr 2010]