ATP12A (NM_001185085) Human Tagged ORF Clone

CAT#: RG230888

ATP12A (GFP-tagged) - Human ATPase, H+/K+ transporting, nongastric, alpha polypeptide (ATP12A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATP12A
• Synonyms: ATP1AL1; HK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230888 representing NM_001185085
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCAGAAAACCCCAGAAATTTACTCCGTGGAGCTCAGCGGAACTAAGGACATCGTGAAAACAGACA
  AGGGGGATGGCAAGGAGAAGTATAGGGGTCTGAAGAACAACTGCCTGGAACTCAAAAAGAAAAATCACAA
  AGAGGAGTTTCAGAAAGAACTCCATCTGGATGACCACAAACTCAGCAATAGGGAATTGGAAGAGAAATAT
  GGCACAGACATCATTATGGGTCTCTCCAGCACCAGAGCTGCCGAGCTCCTGGCCCGGGATGGGCCCAACT
  CCCTCACCCCTCCCAAGCAGACGCCTGAGATCGTCAAGTTCCTCAAGCAGATGGTGGGGGGGTTCTCTAT
  CCTCCTGTGGGTGGGCGCCTTTCTCTGTTGGATTGCATATGGGATTCAGTACTCCAGCGACAAGTCTGCA
  TCCCTGAACAACGTGTACTTGGGCTGTGTGCTTGGTCTGGTGGTCATTTTAACGGGGATCTTTGCTTATT
  ACCAAGAGGCAAAAAGCACCAACATCATGTCCAGCTTCAATAAGATGATCCCTCAGCAAGCTCTCGTCAT
  CCGAGATTCCGAGAAGAAGACCATCCCTTCAGAGCAGCTGGTGGTGGGGGACATTGTGGAGGTCAAAGGA
  GGAGACCAGATCCCTGCAGACATCAGGGTGCTGTCTTCTCAGGGGTGTCGGGTGGATAACTCATCTCTCA
  CGGGGGAGTCTGAGCCCCAGCCCCGCTCCTCTGAGTTTACCCATGAAAACCCCCTGGAAACAAAGAACAT
  CTGCTTCTATTCCACAACGTGTCTGGAAGCATCTACTTCCCCTGTAGGCACTGTCACCGGCATGGTTATC
  AACACGGGTGACCGCACCATCATTGGCCATATTGCCTCATTGGCCTCAGGAGTTGGAAATGAGAAGACGC
  CCATTGCCATTGAGATCGAGCACTTTGTTCACATTGTGGCAGGAGTGGCTGTCTCCATCGGCATCCTTTT
  CTTCATCATCGCTGTGTCCCTGAAGTATCAAGTCCTGGACTCCATCATCTTCCTCATTGGCATCATTGTG
  GCCAATGTGCCCGAGGGCCTCCTGGCCACTGTCACTGTGACCCTGTCGCTGACAGCAAAACGGATGGCCA
  AGAAGAACTGCCTGGTGAAGAACCTGGAGGCTGTGGAGACCCTCGGCTCCACCTCCATCATCTGCTCGGA
  CAAGACTGGGACACTGACCCAGAACAGGATGACAGTGGCCCATCTGTGGTTCGACAATCAGATCTTTGTG
  GCTGACACCAGTGAGGACCATTCAAACCAAGTCTTTGACCAAAGCTCTAGGACTTGGGCCTCCTTATCCA
  AGATAATAACATTGTGTAACCGAGCAGAGTTCAAGCCAGGACAGGAAAATGTCCCCATCATGAAGAAAGC
  TGTGATTGGAGATGCCTCAGAAACTGCTCTTTTAAAATTCTCAGAGGTCATTTTGGGTGATGTGATGGAA
  ATTAGAAAAAGAAACCGCAAAGTAGCTGAAATCCCTTTTAACTCTACTAATAAATTTCAGCTCTCCATCC
  ACGAGATGGATGACCCCCACGGCAAGCGCTTCCTCATGGTGATGAAGGGGGCCCCTGAGCGCATTCTAGA
  GAAATGCAGCACCATCATGATCAACGGCGAGGAGCACCCACTGGACAAGAGCACTGCCAAGACCTTCCAC
  ACAGCCTACATGGAGCTGGGCGGGTTGGGCGAGCGTGTGCTGGGTTTCTGTCATCTCTACCTGCCAGCAG
  ACGAGTTTCCAGAAACCTACTCATTTGACATAGACGCTATGAACTTTCCGACCTCCAACCTCTGTTTTGT
  GGGACTCTTGTCAATGATCGATCCCCCTCGGTCCACCGTGCCAGATGCAGTCACCAAATGCCGGAGTGCA
  GGGATCAAGGTTATTATGGTTACTGGTGATCATCCCATCACAGCCAAAGCTATTGCCAAGAGTGTGGGGA
  TCATTTCAGCCAACAGTGAAACAGTGGAAGACATTGCACATCGCCTCAACATTGCTGTGGAGCAAGTTAA
  CAAACGGGATGCCAAGGCCGCTGTGGTGACTGGCATGGAGCTGAAGGACATGAGCTCAGAACAGCTGGAT
  GAGATCTTAGCCAACTACCAGGAGATTGTCTTTGCCCGGACATCCCCCCAGCAGAAGCTGATCATTGTGG
  AGGGCTGTCAGAGGCAGGATGCTGTTGTTGCTGTGACCGGGGATGGAGTTAATGACTCTCCGGCTCTAAA
  GAAGGCAGACATTGGGATTGCCATGGGGATAGCAGGTTCTGATGCAGCCAAAAATGCAGCCGACATGGTC
  TTGCTGGACGACAACTTCGCATCCATCGTCACAGGGGTGGAGGAAGGTCGCCTGATCTTTGACAACCTCA
  AGAAGACTATTGCTTATTCCCTGACCAAGAACATTGCCGAGCTGTGCCCCTTTCTGATCTACATCATTGT
  CGGGCTCCCCCTGCCCATTGGCACCATCACCATTCTGTTCATTGACTTGGGGACAGACATTATCCCCTCC
  ATTGCCTTGGCGTACGAGAAAGCTGAAAGTGACATCATGAACAGGAAGCCTCGCCACAAGAATAAGGACA
  GGCTGGTGAACCAGCCGCTCGCTGTGTACTCATACCTGCACATTGGCCTCATGCAAGCCCTGGGAGCTTT
  CCTTGTGTATTTCACCGTCTATGCACAAGAGGGCTTTCTGCCCCGCACTCTCATTAACCTGCGGGTAGAA
  TGGGAGAAGGACTACGTGAATGACTTGAAAGACAGCTATGGGCAGGAATGGACAAGGTACCAGAGGGAAT
  ACCTAGAATGGACGGGCTACACGGCTTTCTTTGTTGGCATCCTAGTCCAGCAAATAGCAGATCTGATCAT
  CAGGAAAACCCGGAGGAATTCCATCTTCCAGCAGGGTCTCTTCAGAAATAAAGTCATCTGGGTGGGGATC
  ACCTCACAGATCATCATTGGTCTGATCCTCTCCTATGGCCTCGGAAGTGTCACAGCCTTGAGTTTCACCA
  TGCTTAGGGCTCAGTACTGGTTTGTGGCTGTGCCGCACGCCATCCTGATCTGGGTGTATGATGAGGTGCG
  GAAGCTCTTCATCAGGCTCTACCCTGGAAGCTGGTGGGATAAGAACATGTATTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230888 representing NM_001185085
  Red=Cloning site Green=Tags(s)
  
  MHQKTPEIYSVELSGTKDIVKTDKGDGKEKYRGLKNNCLELKKKNHKEEFQKELHLDDHKLSNRELEEKY
  GTDIIMGLSSTRAAELLARDGPNSLTPPKQTPEIVKFLKQMVGGFSILLWVGAFLCWIAYGIQYSSDKSA
  SLNNVYLGCVLGLVVILTGIFAYYQEAKSTNIMSSFNKMIPQQALVIRDSEKKTIPSEQLVVGDIVEVKG
  GDQIPADIRVLSSQGCRVDNSSLTGESEPQPRSSEFTHENPLETKNICFYSTTCLEASTSPVGTVTGMVI
  NTGDRTIIGHIASLASGVGNEKTPIAIEIEHFVHIVAGVAVSIGILFFIIAVSLKYQVLDSIIFLIGIIV
  ANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFV
  ADTSEDHSNQVFDQSSRTWASLSKIITLCNRAEFKPGQENVPIMKKAVIGDASETALLKFSEVILGDVME
  IRKRNRKVAEIPFNSTNKFQLSIHEMDDPHGKRFLMVMKGAPERILEKCSTIMINGEEHPLDKSTAKTFH
  TAYMELGGLGERVLGFCHLYLPADEFPETYSFDIDAMNFPTSNLCFVGLLSMIDPPRSTVPDAVTKCRSA
  GIKVIMVTGDHPITAKAIAKSVGIISANSETVEDIAHRLNIAVEQVNKRDAKAAVVTGMELKDMSSEQLD
  EILANYQEIVFARTSPQQKLIIVEGCQRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMV
  LLDDNFASIVTGVEEGRLIFDNLKKTIAYSLTKNIAELCPFLIYIIVGLPLPIGTITILFIDLGTDIIPS
  IALAYEKAESDIMNRKPRHKNKDRLVNQPLAVYSYLHIGLMQALGAFLVYFTVYAQEGFLPRTLINLRVE
  WEKDYVNDLKDSYGQEWTRYQREYLEWTGYTAFFVGILVQQIADLIIRKTRRNSIFQQGLFRNKVIWVGI
  TSQIIIGLILSYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEVRKLFIRLYPGSWWDKNMYY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001185085
• ORF Size: 3135 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001185085.1, NP_001172014.1
• RefSeq Size: 3750 bp
• RefSeq ORF: 3138 bp
• Locus ID: 479
• Cytogenetics: 13q12.1-q12.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Oxidative phosphorylation
• Gene Summary: 'The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'