NrCAM (NM_001193584) Human Tagged ORF Clone

CAT#: RG230893

NRCAM (GFP-tagged) - Human neuronal cell adhesion molecule (NRCAM), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NRCAM
• Synonyms: KIAA0343; MGC138845; MGC138846
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230893 representing NM_001193584
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCTTAAAATAATGCCGAAAAAGAAGCGCTTATCTGCGGGCAGAGTGCCCCTGATTCTCTTCCTGT
  GCCAGATGATTAGTGCACTGGAAGTACCTCTTGATCCAAAACTTCTTGAAGACTTGGTACAGCCTCCAAC
  CATCACCCAACAGTCTCCAAAAGATTACATTATTGACCCTCGGGAGAATATTGTAATCCAGTGTGAAGCC
  AAAGGGAAACCGCCCCCAAGCTTTTCCTGGACCCGTAATGGGACTCATTTTGACATCGATAAAGACCCTC
  TGGTCACCATGAAGCCTGGCACAGGAACGCTCATAATTAACATCATGAGCGAAGGGAAAGCTGAGACCTA
  TGAAGGAGTCTATCAGTGTACAGCAAGGAACGAACGCGGAGCTGCAGTTTCTAATAACATTGTTGTCCGC
  CCATCCAGATCACCATTGTGGACCAAAGAAAAACTTGAACCAATCACACTTCAAAGTGGTCAGTCTTTAG
  TACTTCCCTGCAGACCCCCAATTGGATTACCACCACCTATAATATTTTGGATGGATAATTCCTTTCAAAG
  ACTTCCACAAAGTGAGAGAGTTTCTCAAGGTTTGAATGGGGACCTTTATTTTTCCAATGTCCTCCCAGAG
  GACACCCGCGAAGACTATATCTGTTATGCTAGATTTAATCATACTCAAACCATACAGCAGAAGCAACCTA
  TTTCTGTGAAGGTGATTTCAGCTAAATCAAGTAGAGAGAGGCCACCAACATTTTTAACTCCAGAAGGCAA
  TGCAAGTAACAAAGAGGAATTAAGAGGAAATGTGCTTTCACTGGAGTGCATTGCAGAAGGACTGCCTACC
  CCAATTATTTACTGGGCAAAGGAAGATGGAATGCTACCCAAAAACAGGACAGTTTATAAGAACTTTGAGA
  AAACCTTGCAGATCATTCATGTTTCAGAAGCAGACTCTGGAAATTACCAATGTATAGCAAAAAATGCATT
  AGGAGCCATCCACCATACCATTTCTGTTAGAGTTAAAGCGGCTCCATACTGGATCACAGCCCCTCAAAAT
  CTTGTGCTGTCCCCAGGAGAGGATGGGACCTTGATCTGCAGAGCTAATGGCAACCCCAAACCCAGAATTA
  GCTGGTTAACAAATGGAGTCCCAATAGAAATTGCCCCTGATGACCCCAGCAGAAAAATAGATGGCGATAC
  CATTATTTTTTCAAATGTTCAAGAAAGATCAAGTGCAGTATATCAGTGCAATGCCTCTAATGAATATGGA
  TATTTACTGGCAAACGCATTTGTAAATGTGCTGGCTGAGCCACCACGAATCCTCACACCTGCAAACACAC
  TCTACCAGGTCATTGCAAACAGGCCTGCTTTACTAGACTGTGCCTTCTTTGGGTCTCCTCTCCCAACCAT
  CGAGTGGTTTAAAGGAGCTAAAGGAAGTGCTCTTCATGAAGATATTTATGTTTTACATGAAAATGGAACT
  TTGGAAATTCCTGTGGCCCAAAAGGACAGTACAGGAACTTATACGTGTGTTGCAAGGAATAAATTAGGGA
  TGGCAAAGAATGAAGTTCACTTAGAAATCAAAGATGCTACATGGATCGTTAAACAGCCCGAATATGCAGT
  TGTGCAAAGAGGGAGCATGGTGTCCTTTGAATGCAAAGTGAAACATGATCACACCTTATCCCTCACTGTC
  CTGTGGCTGAAGGACAACAGGGAACTGCCCAGTGATGAAAGGTTCACTGTTGACAAGGATCATCTAGTGG
  TAGCTGATGTCAGTGACGATGACAGCGGGACCTACACGTGTGTGGCCAACACCACTCTGGACAGCGTCTC
  CGCCAGCGCTGTGCTTAGCGTTGTTGCTCCTACTCCAACTCCAGCTCCCGTTTACGATGTCCCAAATCCT
  CCCTTTGACTTAGAACTGACAGATCAACTTGACAAAAGTGTTCAGCTGTCATGGACCCCAGGCGATGACA
  ACAATAGCCCCATTACAAAATTCATCATCGAATATGAAGATGCAATGCACAAGCCAGGGCTGTGGCACCA
  CCAAACTGAAGTTTCTGGAACACAGACCACAGCCCAGCTGAAGCTGTCTCCTTACGTGAACTACTCCTTC
  CGCGTGATGGCAGTGAACAGCATTGGGAAGAGCTTGCCCAGCGAGGCCTCTGAGCAGTATTTGACGAAAG
  CCTCAGAACCAGATAAAAACCCCACAGCTGTGGAAGGACTGGGATCAGAGCCTGATAATTTGGTGATTAC
  GTGGAAGCCCTTGAATGGTTTCGAATCTAATGGGCCAGGCCTTCAGTACAAAGTTAGCTGGCGCCAGAAA
  GATGGTGATGATGAATGGACATCTGTGGTTGTGGCAAATGTATCCAAATATATTGTCTCAGGCACGCCAA
  CCTTTGTTCCATACCTGATCAAAGTTCAGGCCCTGAATGACATGGGGTTTGCCCCCGAGCCAGCTGTAGT
  CATGGGACATTCTGGAGAAGACCTCCCAATGGTGGCTCCTGGGAACGTGCGTGTGAATGTGGTGAACAGT
  ACCTTAGCCGAGGTGCACTGGGACCCAGTACCTCTGAAAAGCATCCGAGGACACCTACAAGGCTATCGGA
  TTTACTATTGGAAGACCCAGAGTTCATCTAAAAGAAACAGACGTCACATTGAGAAAAAGATCCTCACCTT
  CCAAGGCAGCAAGACTCATGGCATGTTGCCGGGGCTAGAGCCCTTTAGCCACTACACACTGAATGTCCGA
  GTGGTCAATGGGAAAGGGGAGGGCCCAGCCAGCCCTGACAGAGTCTTTAATACTCCAGAAGGAGTCCCCA
  GTGCTCCCTCGTCTTTGAAGATTGTGAATCCAACACTGGACTCTCTCACTTTGGAATGGGATCCACCGAG
  CCACCCGAATGGCATTTTGACAGAGTACACCTTAAAGTATCAGCCAATTAACAGCACACATGAATTAGGC
  CCTCTGGTAGATTTGAAAATTCCTGCCAACAAGACACGGTGGACTTTAAAAAATTTAAATTTCAGCACTC
  GATATAAGTTTTATTTCTATGCACAAACATCAGCAGGATCAGGAAGTCAAATTACAGAGGAAGCAGTAAC
  AACTGTGGATGAAGCGATGGCAAGCCGGCAGGTGGATATTGCAACTCAGGGCTGGTTCATTGGTCTGATG
  TGTGCTGTTGCTCTCCTTATCTTAATTTTGCTGATTGTTTGCTTCATCAGAAGAAACAAGGGTGGTAAAT
  ATCCAGTTAAAGAAAAGGAAGATGCCCATGCTGACCCTGAAATCCAGCCTATGAAGGAAGATGATGGGAC
  ATTTGGAGAATACAGTGATGCAGAAGACCACAAGCCTTTGAAAAAAGGAAGTCGAACTCCTTCAGACAGG
  ACTGTGAAAAAAGAAGATAGTGACGACAGCCTAGTTGACTATGGAGAAGGGGTTAATGGCCAGTTCAATG
  AGGATGGCTCCTTTATTGGACAATACAGTGGTAAGAAAGAGAAAGAGCCGGCTGAAGGAAACGAAAGCTC
  AGAGGCACCTTCTCCTGTCAACGCCATGAATTCCTTTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230893 representing NM_001193584
  Red=Cloning site Green=Tags(s)
  
  MQLKIMPKKKRLSAGRVPLILFLCQMISALEVPLDPKLLEDLVQPPTITQQSPKDYIIDPRENIVIQCEA
  KGKPPPSFSWTRNGTHFDIDKDPLVTMKPGTGTLIINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVR
  PSRSPLWTKEKLEPITLQSGQSLVLPCRPPIGLPPPIIFWMDNSFQRLPQSERVSQGLNGDLYFSNVLPE
  DTREDYICYARFNHTQTIQQKQPISVKVISAKSSRERPPTFLTPEGNASNKEELRGNVLSLECIAEGLPT
  PIIYWAKEDGMLPKNRTVYKNFEKTLQIIHVSEADSGNYQCIAKNALGAIHHTISVRVKAAPYWITAPQN
  LVLSPGEDGTLICRANGNPKPRISWLTNGVPIEIAPDDPSRKIDGDTIIFSNVQERSSAVYQCNASNEYG
  YLLANAFVNVLAEPPRILTPANTLYQVIANRPALLDCAFFGSPLPTIEWFKGAKGSALHEDIYVLHENGT
  LEIPVAQKDSTGTYTCVARNKLGMAKNEVHLEIKDATWIVKQPEYAVVQRGSMVSFECKVKHDHTLSLTV
  LWLKDNRELPSDERFTVDKDHLVVADVSDDDSGTYTCVANTTLDSVSASAVLSVVAPTPTPAPVYDVPNP
  PFDLELTDQLDKSVQLSWTPGDDNNSPITKFIIEYEDAMHKPGLWHHQTEVSGTQTTAQLKLSPYVNYSF
  RVMAVNSIGKSLPSEASEQYLTKASEPDKNPTAVEGLGSEPDNLVITWKPLNGFESNGPGLQYKVSWRQK
  DGDDEWTSVVVANVSKYIVSGTPTFVPYLIKVQALNDMGFAPEPAVVMGHSGEDLPMVAPGNVRVNVVNS
  TLAEVHWDPVPLKSIRGHLQGYRIYYWKTQSSSKRNRRHIEKKILTFQGSKTHGMLPGLEPFSHYTLNVR
  VVNGKGEGPASPDRVFNTPEGVPSAPSSLKIVNPTLDSLTLEWDPPSHPNGILTEYTLKYQPINSTHELG
  PLVDLKIPANKTRWTLKNLNFSTRYKFYFYAQTSAGSGSQITEEAVTTVDEAMASRQVDIATQGWFIGLM
  CAVALLILILLIVCFIRRNKGGKYPVKEKEDAHADPEIQPMKEDDGTFGEYSDAEDHKPLKKGSRTPSDR
  TVKKEDSDDSLVDYGEGVNGQFNEDGSFIGQYSGKKEKEPAEGNESSEAPSPVNAMNSFV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193584
• ORF Size: 3540 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001193584.1, NP_001180513.1
• RefSeq Size: 6313 bp
• RefSeq ORF: 3543 bp
• Locus ID: 4897
• Cytogenetics: 7q31.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: 'Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'