ARPC4 (NM_001198780) Human Tagged ORF Clone

CAT#: RG231035

ARPC4 (GFP-tagged) - Human actin related protein 2/3 complex, subunit 4, 20kDa (ARPC4), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARPC4
• Synonyms: ARC20; P20-ARC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231035 representing NM_001198780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGAGAGCCGGGCCCCCGGCCAGGGACCCCCGGCTGTTCGGCCTCAGGGCAGTGGACTGCCACTC
  TCCGCCCCTACCTGAGTGCCGTGCGGGCCACATTGCAGGCTGCCCTCTGCCTGGAGAACTTCTCCTCCCA
  GGTTGTGGAACGACACAACAAGCCGGAAGTGGAAGTCAGGAGTAGCAAAGAGCTCCTGTTACAACCTGTG
  ACCATCAGCAGGAATGAGAAGGAAAAGGTTCTGATTGAGGGCTCCATCAACTCTGTCCGGGTCAGCATTG
  CTGTGAAACAGGCTGATGAGATCGAGAAGATTTTGTGCCACAAGTTCATGCGCTTCATGATGATGCGAGC
  AGAGAACTTCTTTATCCTTCGAAGGAAGCCTGTGGAGGGGTATGATATCAGCTTTCTGATCACCAACTTC
  CACACAGAGCAGATGTACAAACACAAGTTGGTGGACTTTGTGATCCACTTCATGGAGGAGATTGACAAGG
  AGATCAGTGAGATGAAGCTGTCAGTCAATGCCCGTGCCCGCATTGTGGCTGAAGAGTTCCTTAAGAATTT
  T
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231035 representing NM_001198780
  Red=Cloning site Green=Tags(s)
  
  MVREPGPRPGTPGCSASGQWTATLRPYLSAVRATLQAALCLENFSSQVVERHNKPEVEVRSSKELLLQPV
  TISRNEKEKVLIEGSINSVRVSIAVKQADEIEKILCHKFMRFMMMRAENFFILRRKPVEGYDISFLITNF
  HTEQMYKHKLVDFVIHFMEEIDKEISEMKLSVNARARIVAEEFLKNF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198780
• ORF Size: 561 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001198780.1, NP_001185709.1
• RefSeq Size: 1476
• RefSeq ORF: 564
• Locus ID: 10093
• Protein Pathways: Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit, which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring read-through transcription exists between this gene and the downstream tubulin tyrosine ligase-like family, member 3 (TTLL3), which results in the production of a fusion protein. [provided by RefSeq, Nov 2010]