Myeloid leukemia factor 1 (MLF1) (NM_001195434) Human Tagged ORF Clone

CAT#: RG231106

MLF1 (GFP-tagged) - Human myeloid leukemia factor 1 (MLF1), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MLF1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231106 representing NM_001195434
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACAGATGATAAGAAGTTTTTCTGAACCCTTTGGAAGAGACTTGCTCAGTATCTCTGATGGTAGAG
  GGAGAGCTCATAATCGTAGAGGACATAATGATGGTGAAGATTCTTTGACTGCAACGAGTTGTTCTCTTGT
  GCCTTTTGGCGATTTTGGTGGTATGCATACAGATGTCAGCTCTTTCCAGACAATGGACCAAATGGTGTCA
  AATATGAGAAACTATATGCAGAAATTAGAAAGAAACTTCGGTCAACTTTCAGTGGATCCAAATGGACATT
  CATTTTGTTCTTCCTCAGTTATGACTTATTCCAAAATAGGAGATGAACCGCCAAAGGTTTTTCAGGCCTC
  AACTCAAACTCGTCGAGCTCCAGGAGGAATAAAGGAAACCAGGAAAGCAATGAGAGATTCTGACAGTGGA
  CTAGAAAAAATGGCTATTGGTCATCATATCCATGACCGAGCTCATGTCATTAAAAAGTCAAAGAACAAGA
  AGACTGGAGATGAAGAGGTCAACCAGGAGTTCATCAATATGAATGAAAGTGATGCTCATGCTTTTGATGA
  GGAGTGGCAAAGTGAGGTTTTGAAGTACAAACCAGGACGACACAATCTAGGAAACACTAGAATGAGAAGT
  GTTGGCCATGAGAATCCTGGCTCCCGAGAACTTAAAAGAAGGGAGAAACCTCAACAAAGTCCAGCCATTG
  AACATGGAAGGAGATCAAATGTTTTGGGGGACAAACTCCACATCAAAGGCTCATCTGTGAAAAGCAACAA
  AAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231106 representing NM_001195434
  Red=Cloning site Green=Tags(s)
  
  MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVS
  NMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSG
  LEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRS
  VGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195434
• ORF Size: 774 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195434.1, NP_001182363.1
• RefSeq Size: 2381 bp
• RefSeq ORF: 777 bp
• Locus ID: 4291
• Cytogenetics: 3q25.32
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]'