Cytochrome P450 2C8 (CYP2C8) (NM_001198854) Human Tagged ORF Clone

CAT#: RG231201

CYP2C8 (GFP-tagged) - Human cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CYP2C8
• Synonyms: CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231201 representing NM_001198854
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTCCAGCCAATTGCAAAGGGAATCATTTCCAGCAATGGAAAGAGATGGAAGGAGATCCGGCGTT
  TCTCCCTCACAACCTTGCGGAATTTTGGGATGGGGAAGAGGAGCATTGAGGACCGTGTTCAAGAGGAAGC
  TCACTGCCTTGTGGAGGAGTTGAGAAAAACCAAGGCTTCACCCTGTGATCCCACTTTCATCCTGGGCTGT
  GCTCCCTGCAATGTGATCTGCTCCGTTGTTTTCCAGAAACGATTTGATTATAAAGATCAGAATTTTCTCA
  CCCTGATGAAAAGATTCAATGAAAACTTCAGGATTCTGAACTCCCCATGGATCCAGGTCTGCAATAATTT
  CCCTCTACTCATTGATTGTTTCCCAGGAACTCACAACAAAGTGCTTAAAAATGTTGCTCTTACACGAAGT
  TACATTAGGGAGAAAGTAAAAGAACACCAAGCATCACTGGATGTTAACAATCCTCGGGACTTTATCGATT
  GCTTCCTGATCAAAATGGAGCAGGAAAAGGACAACCAAAAGTCAGAATTCAATATTGAAAACTTGGTTGG
  CACTGTAGCTGATCTATTTGTTGCTGGAACAGAGACAACAAGCACCACTCTGAGATATGGACTCCTGCTC
  CTGCTGAAGCACCCAGAGGTCACAGCTAAAGTCCAGGAAGAGATTGATCATGTAATTGGCAGACACAGGA
  GCCCCTGCATGCAGGATAGGAGCCACATGCCTTACACTGATGCTGTAGTGCACGAGATCCAGAGATACAG
  TGACCTTGTCCCCACCGGTGTGCCCCATGCAGTGACCACTGATACTAAGTTCAGAAACTACCTCATCCCC
  AAGGGCACAACCATAATGGCATTACTGACTTCCGTGCTACATGATGACAAAGAATTTCCTAATCCAAATA
  TCTTTGACCCTGGCCACTTTCTAGATAAGAATGGCAACTTTAAGAAAAGTGACTACTTCATGCCTTTCTC
  AGCAGGAAAACGAATTTGTGCAGGAGAAGGACTTGCCCGCATGGAGCTATTTTTATTTCTAACCACAATT
  TTACAGAACTTTAACCTGAAATCTGTTGATGATTTAAAGAACCTCAATACTACTGCAGTTACCAAAGGGA
  TTGTTTCTCTGCCACCCTCATACCAGATCTGCTTCATCCCTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231201 representing NM_001198854
  Red=Cloning site Green=Tags(s)
  
  MFLQPIAKGIISSNGKRWKEIRRFSLTTLRNFGMGKRSIEDRVQEEAHCLVEELRKTKASPCDPTFILGC
  APCNVICSVVFQKRFDYKDQNFLTLMKRFNENFRILNSPWIQVCNNFPLLIDCFPGTHNKVLKNVALTRS
  YIREKVKEHQASLDVNNPRDFIDCFLIKMEQEKDNQKSEFNIENLVGTVADLFVAGTETTSTTLRYGLLL
  LLKHPEVTAKVQEEIDHVIGRHRSPCMQDRSHMPYTDAVVHEIQRYSDLVPTGVPHAVTTDTKFRNYLIP
  KGTTIMALLTSVLHDDKEFPNPNIFDPGHFLDKNGNFKKSDYFMPFSAGKRICAGEGLARMELFLFLTTI
  LQNFNLKSVDDLKNLNTTAVTKGIVSLPPSYQICFIPV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198854
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198854.1, NP_001185783.1
• RefSeq Size: 1799 bp
• RefSeq ORF: 1167 bp
• Locus ID: 1558
• Cytogenetics: 10q23.33
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]'