Glutathione Reductase (GSR) (NM_001195102) Human Tagged ORF Clone

CAT#: RG231274

GSR (GFP-tagged) - Human glutathione reductase (GSR), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GSR
• Synonyms: GR; HEL-75; HEL-S-122m
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231274 representing NM_001195102
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGCTGCCCCGAGCCCTGAGCGCCGGCGCGGGACCGAGCTGGCGGCGGGCGGCGCGCGCCTTCC
  GAGGCTTCCTGCTGCTTCTGCCCGAGCCCGCGGCCCTCACGCGCGCCCTCTCCCGTGCCATGGCCTGCAG
  GCAGGAGCCGCAGCCGCAGGGCCCGCCGCCCGCTGCTGGCGCCGTGGCCTCCTATGACTACCTGGTGATC
  GGGGGCGGCTCGGGCGGGCTGGCCAGCGCGCGCAGGGCGGCCGAGCTGGGTGCCAGGGCCGCCGTGGTGG
  AGAGCCACAAGCTGGGTGGCACTTGCGTGAATGTTGGATGTGTACCCAAAAAGGTAATGTGGAACACAGC
  TGTCCACTCTGAATTCATGCATGATCATGCTGATTATGGCTTTCCAAGTTGTGAGGGTAAATTCAATTGG
  CGTGTTATTAAGGAAAAGCGGGATGCCTATGTGAGCCGCCTGAATGCCATCTATCAAAACAATCTCACCA
  AGTCCCATATAGAAATCATCCGTGGCCATGCAGCCTTCACGAGTGATCCCAAGCCCACAATAGAGGTCAG
  TGGGAAAAAGTACACCGCCCCACACATCCTGATCGCCACAGGTGGTATGCCCTCCACCCCTCATGAGAGC
  CAGATCCCCGGTGCCAGCTTAGGAATAACCAGCGATGGATTTTTTCAGCTGGAAGAATTGCCCGGCCGCA
  GCGTCATTGTTGGTGCAGGTTACATTGCTGTGGAGATGGCAGGGATCCTGTCAGCCCTGGGTTCTAAGAC
  ATCACTGATGATACGGCATGATAAGGTCAAGGAGGTTAAAAAGACTTTGTCGGGCTTGGAAGTCAGCATG
  GTTACTGCAGTTCCCGGTAGGCTACCAGTCATGACCATGATTCCAGATGTTGACTGCCTGCTCTGGGCCA
  TTGGGCGGGTCCCGAATACCAAGGACCTGAGTTTAAACAAACTGGGGATTCAAACCGATGACAAGGGTCA
  TATCATCGTAGACGAATTCCAGAATACCAACGTCAAAGGCATCTATGCAGTTGGGGATGTATGTGGAAAA
  GCTCTTCTTACTCCAGTTGCAATAGCTGCTGGCCGAAAACTTGCCCATCGACTTTTTGAATATAAGGAAG
  ATTCCAAATTAGATTATAACAACATCCCAACTGTGGTCTTCAGCCACCCCCCTATTGGGACAGTGGGACT
  CACGGAAGATGAAGCCATTCATAAATATGGAATAGAAAATGTGAAGACCTATTCAACGAGCTTTACCCCG
  ATGTATCACGCAGTTACCAAAAGGAAAACAAAATGTGTGATGAAAATGGTCTGTGCTAACAAGGAAGAAA
  AGGTGGTTGGGATCCATATGCAGGGACTTGGGTGTGATGAAATGCTGCAGGGTTTTGCTGTTGCAGTGAA
  GATGGGAGCAACGAAGGCAGACTTTGACAACACAGTCGCCATTCACCCTACCTCTTCAGAAGAGCTGGTC
  ACACTTCGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231274 representing NM_001195102
  Red=Cloning site Green=Tags(s)
  
  MALLPRALSAGAGPSWRRAARAFRGFLLLLPEPAALTRALSRAMACRQEPQPQGPPPAAGAVASYDYLVI
  GGGSGGLASARRAAELGARAAVVESHKLGGTCVNVGCVPKKVMWNTAVHSEFMHDHADYGFPSCEGKFNW
  RVIKEKRDAYVSRLNAIYQNNLTKSHIEIIRGHAAFTSDPKPTIEVSGKKYTAPHILIATGGMPSTPHES
  QIPGASLGITSDGFFQLEELPGRSVIVGAGYIAVEMAGILSALGSKTSLMIRHDKVKEVKKTLSGLEVSM
  VTAVPGRLPVMTMIPDVDCLLWAIGRVPNTKDLSLNKLGIQTDDKGHIIVDEFQNTNVKGIYAVGDVCGK
  ALLTPVAIAAGRKLAHRLFEYKEDSKLDYNNIPTVVFSHPPIGTVGLTEDEAIHKYGIENVKTYSTSFTP
  MYHAVTKRKTKCVMKMVCANKEEKVVGIHMQGLGCDEMLQGFAVAVKMGATKADFDNTVAIHPTSSEELV
  TLR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195102
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195102.1, NP_001182031.1
• RefSeq Size: 3087 bp
• RefSeq ORF: 1482 bp
• Locus ID: 2936
• Cytogenetics: 8p12
• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism
• Gene Summary: 'This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]'