POLE2 (NM_001197330) Human Tagged ORF Clone

CAT#: RG231276

POLE2 (GFP-tagged) - Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POLE2
• Synonyms: DPE2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231276 representing NM_001197330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGAGCGGCTGCGGAGCCGGGCGCTCTCCGCCTTCAAGTTGCGGGGCTTGCTGCTCCGTGGTG
  AAGCTATTAAGTACCTCACAGAAGCTCTTCAGTCTATCAGTGAATTAGAGCTTGAAGATAAACTGGAAAA
  GATAATTAATGCAGTTGAGAAGCAACCCTTGTCATCAAACATGATTGAACGATCTGTGGTGGAAGCAGCA
  GTCCAGGAATGCAGTCAGTCTGTTGATGAAACTATTCTGTTAATGACCAACCACCCTGCACCAAATTTAT
  TTGGAACACCAAGAGATAAAGCAGAGATGTTTCGTGAGCGATATACCATTTTGCACCAGAGGACCCACAG
  GCATGAATTATTTACTCCTCCGGTGATAGGTTCTCACCCTGATGAAAGCGGAAGCAAATTCCAGCTTAAA
  ACAATAGAAACCTTATTGGGTAGTACAACCAAAATCGGAGATGCGATTGTTCTTGGAATGATAACGCAGT
  TAAAAGAGGGAAAATTTTTTCTGGAAGATCCTACTGGAACAGTCCAACTAGACCTTAGTAAAGCTCAGTT
  CCATAGTGGTTTATACACAGAGGCATGCTTTGTCTTAGCAGAAGGTTGGTTTGAAGATCAAGTGTTTCAT
  GTCAATGCCTTTGGATTTCCACCCACTGAGCCCTCTAGTACTACTAGGGCATACTATGGAAATATTAATT
  TTTTTGGAGGTCCTTCTAATACATCTGTGAAGACTTCTGCAAAACTAAAACAGCTAGAAGAGGAGAATAA
  AGATGCTATGTTTGTGTTTTTATCTGATGTTTGGTTGGACCAGGTGGAAGTATTGGAAAAACTTCGCATA
  ATGTTTGCTGGTTATTCACCAGCACCTCCAACCTGCTTTATTCTGTGTGGTAATTTTTCATCTGCACCAT
  ATGGAAAAAATCAAGTTCAAGCTTTGAAAGATTCCCTAAAAACTTTGGCAGATATAATATGTGAATACCC
  AGATATTCACCAAAGTAGTCGTTTTGTGTTTGTACCTGGTCCAGAGGATCCTGGATTTGGTTCCATCTTA
  CCAAGGCCACCACTTGCTGAAAGCATCACTAATGAATTCAGACAAAGGGTACCATTTTCAGTTTTTACTA
  CTAATCCTTGCAGAATTCAGTACTGTACACAGGAAATTACTGTCTTCCGTGAAGACTTAGTAAATAAAAT
  GTGCAGAAACTGCGTCCGTTTTCCTAGCAGCAATTTGGCTATTCCTAATCACTTTGTAAAGACTATCTTA
  TCCCAAGGACATCTGACTCCCCTACCTCTTTATGTCTGCCCAGTGTATTGGGCATATGACTATGCTTTGA
  GAGTGTATCCTGTGCCCGATCTACTTGTCATTGCAGACAAATATGATCCTTTCACTACGACAAATACCGA
  ATGCCTCTGCATAAACCCTGGCTCTTTTCCAAGAAGTGGATTTTCATTCAAAGTTTTTTATCCTTCTAAT
  AAGACAGTAGAAGATAGCAAACTTCAAGGCTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231276 representing NM_001197330
  Red=Cloning site Green=Tags(s)
  
  MAPERLRSRALSAFKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSNMIERSVVEAA
  VQECSQSVDETILLMTNHPAPNLFGTPRDKAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLK
  TIETLLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFH
  VNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFLSDVWLDQVEVLEKLRI
  MFAGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSIL
  PRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSSNLAIPNHFVKTIL
  SQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSN
  KTVEDSKLQGF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001197330
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001197330.1, NP_001184259.1
• RefSeq Size: 1783
• RefSeq ORF: 1506
• Locus ID: 5427
• Protein Pathways: Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
• Gene Summary: DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. [provided by RefSeq, Jan 2017]