eIF4GII (EIF4G3) (NM_001198803) Human Tagged ORF Clone

CAT#: RG231286

EIF4G3 (GFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EIF4G3
• Synonyms: eIF-4G 3; eIF4G 3; eIF4GII
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231286 representing NM_001198803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTCACAACCTCAAACCCGTTCTCCGTTTGCAGCGGGGCCTCGACCTCCCCATCATCAGTTTTTCC
  AGAGGCCTCAAATACAGCCTCCTAGAGCTACCATCCCGAACAGCAGTCCTTCCATTCGTCCTGGTGCACA
  GACACCCACTGCAGTGTACCAGGCTAATCAGCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCA
  GTGCCCCAGGGGCCTCAGTACTGTATACCACAGTACCGTCATAGTGGCCCTCCTTATGTTGGGCCCCCCC
  AACAATATCCAGTTCAACCACCGGGGCCAGGTCCTTTTTATCCTGGACCAGGACCTGGGGACTTCCCCAA
  TGCTTATGGAACGCCTTTTTACCCAAGTCAGCCGGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAG
  CAACAGCCGCCTCCAGCCAAGAGAGAGAAAAAAACTATAAGAATTCGGGATCCAAACCAGGGAGGTAAAG
  ACATAACAGAGGAGATTATGTCTGGAGGTGGCAGCAGAAATCCTACTCCACCCATAGGAAGACCCACGTC
  CACACCTACTCCTCCTCAGCAGCTGCCCAGCCAGGTCCCCGAGCACAGCCCTGTGGTTTATGGGACTGTG
  GAGAGCGCTCATCTTGCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAGGAGAAGCCAA
  AACCAGATCCAGTGTTAAAGTCTCCTTCCCCAGTCCTTAGGCTAGTCCTCAGTGGAGAGAAGAAAGAACA
  AGAAGGCCAGACATCTGAAACTACTGCAATAGTATCCATAGCAGAGCTTCCTCTGCCTCCATCACCTACC
  ACTGTTTCTTCTGTTGCTCGAAGTACAATTGCAGCCCCCACCTCTTCTGCTCTTAGTAGCCAACCAATAT
  TCACCACTGCTATAGATGACAGATGTGAACTCTCATCCCCAAGAGAAGACACAATTCCTATACCCAGCCT
  CACATCTTGCACAGAAACATCAGACCCTTTACCAACAAATGAAAATGATGATGATATATGCAAGAAACCC
  TGTAGTGTAGCACCTAATGATATTCCACTGGTTTCTAGTACTAACCTAATTAATGAAATAAATGGAGTTA
  GCGAAAAATTATCAGCCACGGAGAGCATTGTGGAAATAGTAAAACAGGAAGTATTGCCATTGACTCTTGA
  ATTGGAGATTCTCGAAAATCCCCCAGAAGAAATGAAACTGGAGTGTATCCCAGCTCCCATCACCCCTTCC
  ACAGTTCCTTCCTTTCCTCCAACTCCTCCAACTCCTCCAGCTTCTCCTCCTCACACTCCAGTCATTGTTC
  CTGCTGCTGCCACTACTGTTAGTTCTCCGAGTGCTGCCATCACAGTCCAGAGAGTCCTAGAGGAGGACGA
  GAGCATAAGAACTTGCCTTAGTGAAGATGCAAAAGAGATTCAGAACAAAATAGAGGTAGAAGCAGATGGG
  CAAACAGAAGAGATTTTGGATTCTCAAAACTTAAATTCAAGAAGGAGCCCTGTCCCAGAAACATCGAATG
  AATGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231286 representing NM_001198803
  Red=Cloning site Green=Tags(s)
  
  MNSQPQTRSPFAAGPRPPHHQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYP
  VPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQ
  QQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTV
  ESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPT
  TVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDDDICKKP
  CSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPS
  TVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADG
  QTEEILDSQNLNSRRSPVPETSNEC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198803
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001198803.1, NP_001185732.1
• RefSeq Size: 2213
• RefSeq ORF: 1548
• Locus ID: 8672
• Protein Families: Transcription Factors
• Protein Pathways: Viral myocarditis
• Gene Summary: The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. [provided by RefSeq, May 2016]