NT5C1B (NM_001199086) Human Tagged ORF Clone

CAT#: RG231329

NT5C1B (GFP-tagged) - Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NT5C1B
• Synonyms: AIRP; CN-IB; CN1B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231329 representing NM_001199086
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGTCCTCAAAAGAGAGTCTAGAAG
  CAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTCTGAGCAATCAGGGTCACTCGTCGTGTAGACG
  CTGCCTTTGTGCAGCTGAGGGAACAGCCCTTGGCCCCTGCCACACAATACGTATTTATATTCACATGTGC
  CTGTTGTGGGAGCAGGGCCAGCAGATCACCATGATGAGGGGATCACAAGAATCATCACTGCGGAAGACAG
  ACTCTCGAGGGTACCTTGTGCGCAGTCAATGGTCTAGAATATCCCGGAGCCCATCCACCAAGGCTCCATC
  CATAGATGAGCCTAGAAGCAGGAACACCAGTGCTAAGCTCCCCAGCAGCTCCACGAGCTCCCGGACTCCA
  TCCACCTCCCCAAGCCTGCATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCCCTCGCTCCAGCCACCCG
  CGTCGCCCCAGCTGCCCCGGTCGCTGGACTCGCGGCCTCCCACGCCCCCAGAGCCCGATCCTGGCTCCCG
  GCGCAGCACCAAAATGCAAGAGAATCCGGAGGCCTGGGCCCAAGGCATCGTGCGGGAAATCCGCCAGACC
  CGGGACTCGCAGCCGCTGGAATATTCGCGCACGTCCCCCACCGAGTGGAAGTCCTCCAGCCAGCGCAGGG
  GGATCTACCCCGCCTCCACCCAGCTGGACCGCAACTCTCTGTCCGAGCAGCAGCAGCAGCAGCGGGAGGA
  CGAGGACGACTACGAGGCTGCCTACTGGGCATCCATGAGGTCGTTCTACGAGAAGAACCCGAGCTGCTCG
  CGCCCCTGGCCGCCCAAACCCAAGAACGCCATCACCATTGCTCTCTCATCCTGCGCGCTCTTCAACATGG
  TGGACGGCAGGAAAATCTACGAGCAAGAGGGTCTGGAAAAGTACATGGAGTATCAGCTCACCAATGAGAA
  CGTCATCCTGACCCCGGGCCCGGCGTTCCGCTTCGTCAAGGCACTACAGTATGTCAATGCTAGACTCCGT
  GATCTATATCCTGATGAACAGGACTTATTTGATATTGTACTGATGACTAATAACCATGCCCAAGTGGGAG
  TGCGGCTTATAAACAGCGTCAATCACTACGGCTTACTGATTGACCGCTTCTGTCTGACCGGGGGAAAAGA
  CCCCATTGGCTATTTGAAGGCATATCTTACCAACTTGTATATTGCTGCAGATTCTGAAAAAGTGCAAGAG
  GCAATACAAGAAGGTATTGCCTCTGCGACAATGTTTGATGGAGCCAAAGACATGGCTTACTGTGACACTC
  AGCTCCGTGTAGCCTTTGATGGGGATGCTGTCCTCTTCTCTGATGAGTCTGAACATTTTACCAAGGAGCA
  TGGGCTCGACAAATTCTTCCAGTATGATACATTATGTGAAAGTAAGCCTCTTGCTCAGGGTCCCCTAAAA
  GGCTTTCTGGAAGATTTAGGCAGACTGCAAAAGAAGTTCTATGCCAAAAATGAACGGTTACTTTGTCCTA
  TCAGGACCTACCTGGTTACAGCTAGGAGTGCAGCCAGTTCAGGCGCCCGTGTGCTGAAGACCCTTCGACG
  CTGGGGTCTAGAGATAGACGAAGCTCTTTTCCTTGCTGGAGCCCCCAAAAGTCCCATCTTGGTGAAGATC
  CGGCCCCACATCTTCTTTGATGACCACATGTTCCACATTGAAGGGGCACAGAGGTTAGGTTCCATCGCAG
  CTTATGGCTTTAATAAAAAATTCAGTAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231329 representing NM_001199086
  Red=Cloning site Green=Tags(s)
  
  MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTGVRLSNQGHSSCRRCLCAAEGTALGPCHTIRIYIHMC
  LLWEQGQQITMMRGSQESSLRKTDSRGYLVRSQWSRISRSPSTKAPSIDEPRSRNTSAKLPSSSTSSRTP
  STSPSLHDSSPPPLSGQPSLQPPASPQLPRSLDSRPPTPPEPDPGSRRSTKMQENPEAWAQGIVREIRQT
  RDSQPLEYSRTSPTEWKSSSQRRGIYPASTQLDRNSLSEQQQQQREDEDDYEAAYWASMRSFYEKNPSCS
  RPWPPKPKNAITIALSSCALFNMVDGRKIYEQEGLEKYMEYQLTNENVILTPGPAFRFVKALQYVNARLR
  DLYPDEQDLFDIVLMTNNHAQVGVRLINSVNHYGLLIDRFCLTGGKDPIGYLKAYLTNLYIAADSEKVQE
  AIQEGIASATMFDGAKDMAYCDTQLRVAFDGDAVLFSDESEHFTKEHGLDKFFQYDTLCESKPLAQGPLK
  GFLEDLGRLQKKFYAKNERLLCPIRTYLVTARSAASSGARVLKTLRRWGLEIDEALFLAGAPKSPILVKI
  RPHIFFDDHMFHIEGAQRLGSIAAYGFNKKFSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199086
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001199086.1, NP_001186015.1
• RefSeq Size: 2847
• RefSeq ORF: 1782
• Locus ID: 93034
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• Gene Summary: Cytosolic 5-prime nucleotidases, such as NT5C1B, catalyze production of adenosine, which regulates diverse physiologic processes (Sala-Newby and Newby, 2001 [PubMed 11690631]). [supplied by OMIM, Mar 2008]