Carboxylesterase 7 (CES5A) (NM_001190158) Human Tagged ORF Clone

CAT#: RG231334

CES5A (GFP-tagged) - Human carboxylesterase 5A (CES5A), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CES5A
• Synonyms: CAUXIN; CES4C1; CES5; CES7; HEL126
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231334 representing NM_001190158
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTTCTGGTTTGCCCTGCTTCATGCCATGGCCTAAAGGAGTTTAGGATAAGAAGGGGAATGTGGA
  GATTGTGCCTGGTTTACTATTTTTATCCAGCCTCCTCAACTTTGTATGTATTGAGGATTGATGTCTTAAA
  CTACACTTCCAAAGATGAGGGGCCTTCTGCTGAAGGGCCACAGAGGAACACCAGGCTGGGATGGATTCAG
  GGCAAGCAAGTCACTGTGCTGGGAAGCCCTGTGCCTGTGAACGTGTTCCTCGGAGTCCCCTTTGCTGCTC
  CCCCGCTGGGATCCCTGCGATTTACGAACCCGCAGCCTGCATCGCCCTGGGATAACTTGCGAGAAGCCAC
  CTCCTACCCTAATTTGTGCCTCCAGAACTCAGAGTGGCTGCTCTTAGATCAACATATGCTCAAGGTGCAT
  TACCCGAAATTCGGAGTGTCAGAAGACTGCCTCTACCTGAACATCTATGCGCCTGCCCACGCCGATACAG
  GCTCCAAGCTCCCCGTCTTGGTGTGGTTCCCAGGAGGTGCCTTCAAGACTGGCTCAGCCTCCATCTTTGA
  TGGGTCCGCCCTGGCTGCCTATGAGGACGTGCTGGTTGTGGTCGTCCAGTACCGGCTAGGAATATTTGGT
  TTCTTCACCACATGGGATCAGCATGCTCCGGGGAACTGGGCCTTCAAGGACCAGGTGGCTGCTCTGTCCT
  GGGTCCAGAAGAACATCGAGTTCTTCGGTGGGGACCCCAGCTCTGTGACCATCTTTGGCGAGTCCGCGGG
  AGCCATAAGTGTTTCTAGTCTTATACTGTCTCCCATGGCCAAAGGCTTATTCCACAAAGCCATCATGGAG
  AGTGGGGTGGCCATCATCCCTTACCTGGAGGCCCATGATTATGAGAAGAGTGAGGACCTGCAGGTGGTTG
  CACATTTCTGTGGTAACAATGCGTCAGACTCTGAGGCCCTGCTGAGGTGCCTGAGGACAAAACCCTCCAA
  GGAGCTGCTGACCCTCAGCCAGAAAACAAAGTCTTTCACTCGAGTGGTTGATGGTGCTTTCTTTCCTAAT
  GAGCCTCTAGATCTATTGTCTCAGAAAGCATTTAAAGCAATTCCTTCCATCATCGGAGTCAATAACCACG
  AGTGTGGCTTCCTGCTGCCTATGAAGGAGGCTCCTGAGATCCTCAGTGGCTCCAACAAGTCCCTTGCCCT
  CCATCTGATACAAAACATCCTGCACATCCCGCCTCAGTATTTGCACCTTGTGGCTAATGAATACTTCCAT
  GACAAGCACTCCCTGACTGAAATCCGAGACAGTCTTCTGGACTTGCTTGGAGATGTGTTCTTTGTGGTCC
  CTGCACTGATCACAGCTCGATATCACAGAGATGCTGGTGCACCTGTCTACTTCTATGAGTTTCGGCACCG
  GCCTCAGTGCTTTGAAGACACGAAGCCAGCTTTTGTCAAAGCCGACCACGCTGATGAAGTCCGCTTTGTG
  TTCGGTGGTGCCTTCCTGAAGGGGGACATTGTTATGTTCGAAGGAGCCACGGAGGAGGAGAAGTTACTGA
  GCCGGAAGATGATGAAATACTGGGCTACCTTTGCTCGAACCGGGAATCCTAATGGGAACGACCTGTCTCT
  GTGGCCAGCTTATAATCTGACTGAGCAGTACCTCCAGCTGGACTTGAACATGAGCCTCGGACAGAGACTC
  AAAGAACCGCGGGTGGATTTTTGGACCAGCACCATCCCCCTGATCCTGTCTGCCTCCGACATGCTCCACA
  GTCCTCTTTCTTCCTTAACTTTCCTCTCTCTCCTCCAGCCTTTCTTTTTCTTTTGTGCTCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231334 representing NM_001190158
  Red=Cloning site Green=Tags(s)
  
  MAVLVCPASCHGLKEFRIRRGMWRLCLVYYFYPASSTLYVLRIDVLNYTSKDEGPSAEGPQRNTRLGWIQ
  GKQVTVLGSPVPVNVFLGVPFAAPPLGSLRFTNPQPASPWDNLREATSYPNLCLQNSEWLLLDQHMLKVH
  YPKFGVSEDCLYLNIYAPAHADTGSKLPVLVWFPGGAFKTGSASIFDGSALAAYEDVLVVVVQYRLGIFG
  FFTTWDQHAPGNWAFKDQVAALSWVQKNIEFFGGDPSSVTIFGESAGAISVSSLILSPMAKGLFHKAIME
  SGVAIIPYLEAHDYEKSEDLQVVAHFCGNNASDSEALLRCLRTKPSKELLTLSQKTKSFTRVVDGAFFPN
  EPLDLLSQKAFKAIPSIIGVNNHECGFLLPMKEAPEILSGSNKSLALHLIQNILHIPPQYLHLVANEYFH
  DKHSLTEIRDSLLDLLGDVFFVVPALITARYHRDAGAPVYFYEFRHRPQCFEDTKPAFVKADHADEVRFV
  FGGAFLKGDIVMFEGATEEEKLLSRKMMKYWATFARTGNPNGNDLSLWPAYNLTEQYLQLDLNMSLGQRL
  KEPRVDFWTSTIPLILSASDMLHSPLSSLTFLSLLQPFFFFCAP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190158
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001190158.1, NP_001177087.1
• RefSeq Size: 2258
• RefSeq ORF: 1815
• Locus ID: 221223
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes
• Gene Summary: This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They also participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene, also called CES5, is predominantly expressed in peripheral tissues, including brain, kidney, lung and testis. It encodes a secreted enzyme. Because of high levels in the urine of male domestic cats, this enzyme is also called cauxin (carboxylesterase-like urinary excreted protein). The enzyme functions in regulating the production of a pheromone precursor and may contribute to lipid and cholesterol transfer processes within male reproductive fluids. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]