NT5C1B (NM_001199103) Human Tagged ORF Clone

CAT#: RG231355

NT5C1B (GFP-tagged) - Human NT5C1B-RDH14 readthrough (NT5C1B-RDH14), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NT5C1B
• Synonyms: AIRP; cN-IB; cN1B; NT5C1B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231355 representing NM_001199103
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGTCCTCAAAAGAGAGTCTAGAAG
  CAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTCTGAGCAATCAGGGATCACAAGAATCATCACT
  GCGGAAGACAGACTCTCGAGGGTACCTTGTGCGCAGTCAATGGTCTAGAATATCCCGGAGCCCATCCACC
  AAGGCTCCATCCATAGATGAGCCTAGAAGCAGGAACACCAGTGCTAAGGTAGAGCTCCCCAGCAGCTCCA
  CGAGCTCCCGGACTCCATCCACCTCCCCAAGCCTGCATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCC
  CTCGCTCCAGCCACCCGCGTCGCCCCAGCTGCCCCGGTCGCTGGACTCGCGGCCTCCCACGCCCCCAGAG
  CCCGATCCTGGCTCCCGGCGCAGCACCAAAATGCAAGAGAATCCGGAGGCCTGGGCCCAAGGCATCGTGC
  GGGAAATCCGCCAGACCCGGGACTCGCAGCCGCTGGAATATTCGCGCACGTCCCCCACCGAGTGGAAGTC
  CTCCAGCCAGCGCAGGGGGATCTACCCCGCCTCCACCCAGCTGGACCGCAACTCTCTGTCCGAGCAGCAG
  CAGCAGCAGCGGGAGGACGAGGACGACTACGAGGCTGCCTACTGGGCATCCATGAGGTCGTTCTACGAGA
  AGAACCCGAGCTGCTCGCGCCCCTGGCCGCCCAAACCCAAGAACGCCATCACCATTGCTCTCTCATCCTG
  CGCGCTCTTCAACATGGTGGACGGCAGGAAAATCTACGAGCAAGAGGGTCTGGAAAAGTACATGGAGTAT
  CAGCTCACCAATGAGAACGTCATCCTGACCCCGGGCCCGGCGTTCCGCTTCGTCAAGGCACTACAGTATG
  TCAATGCTAGACTCCGTGATCTATATCCTGATGAACAGGACTTATTTGATATTGTACTGATGACTAATAA
  CCATGCCCAAGTGGGAGTGCGGCTTATAAACAGCGTCAATCACTACGGCTTACTGATTGACCGCTTCTGT
  CTGACCGGGGGAAAAGACCCCATTGGCTATTTGAAGGCATATCTTACCAACTTGTATATTGCTGCAGATT
  CTGAAAAAGTGCAAGAGGCAATACAAGAAGGTATTGCCTCTGCGACAATGTTTGATGGAGCCAAAGACAT
  GGCTTACTGTGACACTCAGCTCCGTGTAGCCTTTGATGGGGATGCTGTCCTCTTCTCTGATGAGTCTGAA
  CATTTTACCAAGGAGCATGGGCTCGACAAATTCTTCCAGTATGATACATTATGTGAAAGTAAGCCTCTTG
  CTCAGGAAGAGCCTAGGCTGGATGTCTTGATCAATAACGCAGGGATCTTCCAGTGCCCTTACATGAAGAC
  TGAAGATGGGTTTGAGATGCAGTTCGGAGTGAACCATCTGGGGCACTTTCTACTCACCAATCTTCTCCTT
  GGACTCCTCAAAAGTTCAGCTCCCAGCAGGATTGTGGTAGTTTCTTCCAAACTTTATAAATACGGAGACA
  TCAATTTTGATGACTTGAACAGTGAACAAAGCTATAATAAAAGCTTTTGTTATAGCCGGAGCAAACTGGC
  TAACATTCTTTTTACCAGGGAACTAGCCCGCCGCTTAGAAGGCACAAATGTCACCGTCAATGTGTTGCAT
  CCTGGTATTGTACGGACAAATCTGGGGAGGCACATACACATTCCACTGTTGGTCAAACCACTCTTCAATT
  TGGTGTCATGGGCTTTTTTCAAAACTCCAGTAGAAGGTGCCCAGACTTCCATTTATTTGGCCTCTTCACC
  TGAGGTAGAAGGAGTGTCAGGAAGATACTTTGGGGATTGTAAAGAGGAAGAACTGTTGCCCAAAGCTATG
  GATGAATCTGTTGCAAGAAAACTCTGGGATATCAGTGAAGTGATGGTTGGCCTGCTAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231355 representing NM_001199103
  Red=Cloning site Green=Tags(s)
  
  MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTGVRLSNQGSQESSLRKTDSRGYLVRSQWSRISRSPST
  KAPSIDEPRSRNTSAKVELPSSSTSSRTPSTSPSLHDSSPPPLSGQPSLQPPASPQLPRSLDSRPPTPPE
  PDPGSRRSTKMQENPEAWAQGIVREIRQTRDSQPLEYSRTSPTEWKSSSQRRGIYPASTQLDRNSLSEQQ
  QQQREDEDDYEAAYWASMRSFYEKNPSCSRPWPPKPKNAITIALSSCALFNMVDGRKIYEQEGLEKYMEY
  QLTNENVILTPGPAFRFVKALQYVNARLRDLYPDEQDLFDIVLMTNNHAQVGVRLINSVNHYGLLIDRFC
  LTGGKDPIGYLKAYLTNLYIAADSEKVQEAIQEGIASATMFDGAKDMAYCDTQLRVAFDGDAVLFSDESE
  HFTKEHGLDKFFQYDTLCESKPLAQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVNHLGHFLLTNLLL
  GLLKSSAPSRIVVVSSKLYKYGDINFDDLNSEQSYNKSFCYSRSKLANILFTRELARRLEGTNVTVNVLH
  PGIVRTNLGRHIHIPLLVKPLFNLVSWAFFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLPKAM
  DESVARKLWDISEVMVGLLK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199103
• ORF Size: 1950 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001199103.1, NP_001186032.1
• RefSeq Size: 2533
• RefSeq ORF: 1953
• Locus ID: 100526794
• Gene Summary: This locus represents naturally occurring read-through transcription between the neighboring NT5C1B (5'-nucleotidase, cytosolic IB) and RDH14 (retinol dehydrogenase 14) genes on chromosome 2. Alternative splicing results in multiple transcript variants, one of which encodes a fusion protein that shares sequence identity with the products of each individual gene. [provided by RefSeq, Nov 2010]