CNOT4 (NM_001190849) Human Tagged ORF Clone

CAT#: RG231387

CNOT4 (GFP-tagged) - Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CNOT4
• Synonyms: CLONE243; NOT4; NOT4H
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231387 representing NM_001190849
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGCAGTCCTGATGCGAAGGAAGACCCTGTGGAGTGCCCTCTTTGCATGGAGCCCTTGGAGATAG
  ATGATATCAACTTTTTCCCTTGCACCTGTGGCTACCAGATTTGCCGATTTTGTTGGCATCGAATTCGCAC
  TGATGAAAATGGGCTTTGTCCTGCATGTAGAAAGCCATATCCAGAAGACCCAGCAGTTTATAAACCACTC
  TCCCAGGAAGAGCTGCAAAGGATAAAGAATGAGAAAAAACAGAAACAAAATGAGAGAAAACAGAAAATAT
  CAGAAAATCGCAAACATTTGGCTAGTGTACGTGTCGTACAAAAAAACCTCGTCTTTGTTGTAGGTTTATC
  TCAGCGCCTAGCAGACCCAGAGGTTTTAAAACGACCAGAATATTTTGGGAAGTTTGGTAAAATACATAAA
  GTTGTCATCAATAATAGCACATCATATGCAGGCTCACAGGGTCCAAGTGCCAGTGCTTATGTAACCTATA
  TCCGGTCAGAAGACGCTCTCAGAGCCATACAGTGTGTCAACAATGTGGTAGTAGATGGCAGAACACTTAA
  GGCATCTCTAGGTACAACAAAATACTGCAGTTACTTCTTAAAGAATATGCAGTGTCCAAAACCTGACTGC
  ATGTATCTTCATGAATTGGGGGATGAGGCGGCCAGCTTCACAAAAGAGGAAATGCAGGCGGGTAAACACC
  AAGAATATGAACAGAAGCTACTTCAAGAATTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGG
  TTCAGTTGATAAAAATAAGAACAAAGTGACACCACTGCAGAGCCCCATTGACAAACCTTCAGATTCTCTC
  AGTATAGGGAACGGTGATAATTCCCAGCAGATATCTAACAGTGATACGCCTTCACCACCACCTGGTTTGT
  CAAAATCCAATCCAGTCATCCCCATCAGTTCATCCAATCACAGTGCACGGTCCCCTTTTGAAGGGGCAGT
  AACAGAGTCACAGTCGTTATTCTCAGACAATTTTCGCCATCCCAACCCTATCCCAAGTGGGCTTCCTCCT
  TTCCCCAGCTCCCCACAGACATCCAGTGACTGGCCTACAGCACCAGAACCACAGAGCCTCTTCACATCAG
  AAACAATCCCAGTATCATCCTCTACAGACTGGCAAGCAGCTTTTGGCTTTGGTTCTTCTAAACAACCAGA
  GGATGACTTGGGTTTTGATCCCTTCGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAGAAGGAACTG
  TCCGTTCAAGACCAACCTTCCCTTTCGCCCACATCTCTTCAGAACTCCTCTTCACACACTACAACCGCCA
  AAGGTCCAGGCTCTGGATTCCTGCATCCTGCTGCAGCTACAAATGCCAATTCTCTCAATAGTACCTTTTC
  AGTCTTGCCCCAGAGGTTCCCTCAATTTCAGCAGCACCGAGCGGTTTATAATTCATTCAGTTTTCCAGGC
  CAGGCAGCCCGCTATCCTTGGATGGCCTTTCCACGCAATAGCATCATGCACTTGAACCACACAGCAAACC
  CCACCTCAAATAGTAATTTCTTGGACTTGAATCTCCCGCCACAGCACAACACAGGTCTGGGAGGGATCCC
  TGTAGCAGACAACAGCAGTTCTGTAGAGAGTTTAAATATGAAGGAATGGCAGGACGGGCTAAGGGCACTT
  CTACCCAACATTAACATCAACTTTGGTGGACTGCCCAATTCTTCTTCCCCCTCCAACGCCAACCACAGTG
  CACCAACGTCCAACACTGCCACCACCGACAGCCTGAGTTGGGACAGCCCTGGCAGCTGGACAGACCCAGC
  CATCATCACAGGTATTCCAGCGTCTTCAGGAAACAGTTTAGACTCTCTTCAAGATGACAATCCTCCACAC
  TGGCTAAAATCCCTTCAGGCCCTCACAGAGATGGACGGCCCCAGCGCTGCTCCATCACAGACCCACCACA
  GCGCCCCCTTCAGCACACAGATCCCGCTGCACAGAGCCAGTTGGAATCCCTACCCTCCTCCTTCAAACCC
  TTCCAGCTTCCACTCCCCACCCCCAGGCTTTCAGACAGCCTTCAGACCCCCCAGCAAAACCCCCACAGAT
  TTACTACAGAGTTCAACACTGGACCGCCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231387 representing NM_001190849
  Red=Cloning site Green=Tags(s)
  
  MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPEDPAVYKPL
  SQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRLADPEVLKRPEYFGKFGKIHK
  VVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKNMQCPKPDC
  MYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQSPIDKPSDSL
  SIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPP
  FPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKEL
  SVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPG
  QAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVADNSSSVESLNMKEWQDGLRAL
  LPNININFGGLPNSSSPSNANHSAPTSNTATTDSLSWDSPGSWTDPAIITGIPASSGNSLDSLQDDNPPH
  WLKSLQALTEMDGPSAAPSQTHHSAPFSTQIPLHRASWNPYPPPSNPSSFHSPPPGFQTAFRPPSKTPTD
  LLQSSTLDRH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190849
• ORF Size: 2130 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190849.1, NP_001177778.1
• RefSeq Size: 3554 bp
• RefSeq ORF: 2133 bp
• Locus ID: 4850
• Cytogenetics: 7q33
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: 'The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]'