PC6 (PCSK5) (NM_001190482) Human Tagged ORF Clone

CAT#: RG231494

PCSK5 (GFP-tagged) - Human proprotein convertase subtilisin/kexin type 5 (PCSK5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PCSK5
• Synonyms: PC5; PC6; PC6A; SPC6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231494 representing NM_001190482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGGGGGAGCCGCTGCTGCTGCCCGGGACGTTTGGACCTGCTGTGCGTGCTGGCGCTGCTCGGGG
  GCTGCCTGCTCCCCGTGTGTCGGACGCGCGTCTACACCAACCACTGGGCAGTCAAAATCGCCGGGGGCTT
  CCCGGAGGCCAACCGTATCGCCAGCAAGTACGGATTCATCAACATAGGACAGATAGGGGCCCTGAAGGAC
  TACTACCACTTCTACCATAGCAGGACGATTAAAAGGTCAGTTATCTCGAGCAGAGGGACCCACAGTTTCA
  TTTCAATGGAACCAAAGGTGGAATGGATCCAACAGCAAGTGGTAAAAAAGCGGACAAAGAGGGATTATGA
  CTTCAGTCGTGCCCAGTCTACCTATTTCAATGATCCCAAGTGGCCCAGCATGTGGTATATGCACTGCAGT
  GACAATACACATCCCTGCCAGTCTGACATGAATATCGAAGGAGCCTGGAAGAGAGGCTACACGGGAAAGA
  ACATTGTGGTCACTATCCTGGATGACGGAATTGAGAGAACCCATCCAGATCTGATGCAAAACTACGATGC
  TCTGGCAAGTTGCGACGTGAATGGGAATGACTTGGACCCAATGCCTCGTTATGATGCAAGCAACGAGAAC
  AAGCATGGGACTCGCTGTGCTGGAGAAGTGGCAGCCGCTGCAAACAATTCGCACTGCACAGTCGGAATTG
  CTTTCAACGCCAAGATCGGAGGAGTGCGAATGCTGGACGGAGATGTCACGGACATGGTTGAAGCAAAATC
  AGTTAGCTTCAACCCCCAGCACGTGCACATTTACAGCGCCAGCTGGGGCCCGGATGATGATGGCAAGACT
  GTGGACGGACCAGCCCCCCTCACCCGGCAAGCCTTTGAAAACGGCGTTAGAATGGGGCGGAGAGGCCTCG
  GCTCTGTGTTTGTTTGGGCATCTGGAAATGGTGGAAGGAGCAAAGACCACTGCTCCTGTGATGGCTACAC
  CAACAGCATCTACACCATCTCCATCAGCAGCACTGCAGAAAGCGGAAAGAAACCTTGGTACCTGGAAGAG
  TGTTCATCCACGCTGGCCACAACCTACAGCAGCGGGGAGTCCTACGATAAGAAAATCATCACTACAGATC
  TGAGGCAGCGTTGCACGGACAACCACACTGGGACGTCAGCCTCAGCCCCCATGGCTGCAGGCATCATTGC
  GCTGGCCCTGGAAGCCAATCCGTTTCTGACCTGGAGAGACGTACAGCATGTTATTGTCAGGACTTCCCGT
  GCGGGACATTTGAACGCTAATGACTGGAAAACCAATGCTGCTGGTTTTAAGGTGAGCCATCTTTATGGAT
  TTGGACTGATGGACGCAGAAGCCATGGTGATGGAGGCAGAGAAGTGGACCACCGTTCCCCGGCAGCACGT
  GTGTGTGGAGAGCACAGACCGACAAATCAAGACAATCCGCCCTAACAGTGCAGTGCGCTCCATCTACAAA
  GCTTCAGGCTGCTCGGATAACCCCAACCGCCATGTCAACTACCTGGAGCACGTCGTTGTGCGCATCACCA
  TCACCCACCCCAGGAGAGGAGACCTGGCCATCTACCTGACCTCGCCCTCTGGAACTAGGTCTCAGCTTTT
  GGCCAACAGGCTATTTGATCACTCCATGGAAGGATTCAAAAACTGGGAGTTCATGACCATTCATTGCTGG
  GGAGAAAGAGCTGCTGGTGACTGGGTCCTTGAAGTTTATGATACTCCCTCTCAGCTAAGGAACTTTAAGA
  CTCCAGGTAAATTGAAAGAATGGTCTTTGGTCCTCTACGGCACCTCCGTGCAGCCATATTCACCAACCAA
  TGAATTTCCGAAAGTGGAACGGTTCCGCTATAGCCGAGTTGAAGACCCCACAGACGACTATGGCACAGAG
  GATTATGCAGGTCCCTGCGACCCTGAGTGCAGTGAGGTTGGCTGTGACGGGCCAGGACCAGACCACTGCA
  ATGACTGTTTGCACTACTACTACAAGCTGAAAAACAATACCAGGATCTGTGTCTCCAGCTGCCCCCCTGG
  CCACTACCACGCCGACAAGAAGCGCTGCAGGAAGTGTGCCCCCAACTGTGAGTCCTGCTTTGGGAGCCAT
  GGTGACCAATGCATGTCCTGCAAATATGGATACTTTCTGAATGAAGAAACCAACAGCTGTGTTACTCACT
  GCCCTGATGGGTCATATCAGGATACCAAGAAAAATCTTTGCCGGAAATGCAGTGAAAACTGCAAGACATG
  TACTGAATTCCATAACTGTACAGAATGTAGGGATGGGTTAAGCCTGCAGGGATCCCGGTGCTCTGTCTCC
  TGTGAAGATGGACGGTATTTCAACGGCCAGGACTGCCAGCCCTGCCACCGCTTCTGCGCCACTTGTGCTG
  GGGCAGGAGCTGATGGGTGCATTAACTGCACAGAGGGCTACTTCATGGAGGATGGGAGATGCGTGCAGAG
  CTGTAGTATCAGCTATTACTTTGACCACTCTTCAGAGAATGGATACAAATCCTGCAAAAAATGTGATATC
  AGTTGTTTGACGTGCAATGGCCCAGGATTCAAGAACTGTACAAGCTGCCCTAGTGGGTATCTCTTAGACT
  TAGGAATGTGTCAAATGGGAGCCATTTGCAAGGATGGAGAATATGTTGATGAGCATGGCCACTGCCAGAC
  CTGTGAGGCCTCATGTGCCAAGTGCCAGGGACCAACCCAGGAAGACTGCACTACCTGCCCCATGACAAGG
  ATTTTTGATGATGGCCGCTGTGTTTCGAACTGCCCCTCATGGAAATTTGAATTTGAGAACCAATGCCATC
  CATGCCACCACACCTGCCAGAGATGCCAAGGAAGTGGCCCTACCCACTGCACCTCCTGTGGAGCAGACAA
  CTATGGCCGAGAGCACTTCCTGTACCAGGGAGAGTGTGGAGATAGCTGCCCAGAGGGCCACTATGCCACT
  GAGGGGAACACCTGCCTGCCCTGCCCAGACAACTGTGAGCTTTGCCACAGCGTGCATGTCTGCACAAGAT
  GCATGAAGGGCTACTTCATAGCGCCCACCAACCACACATGCCAGAAGTTAGAGTGTGGACAAGGTGAAGT
  CCAAGACCCAGACTATGAAGAATGTGTCCCTTGTGAAGAAGGATGTCTGGGATGCAGCTTGGATGATCCA
  GGAACATGTACATCTTGCGCTATGGGGTATTACAGGTTTGATCACCATTGTTATAAAACCTGTCCTGAGA
  AGACCTACAGTGAGGAAGTGGAATGCAAGGCGTGTGATAGTAACTGTGGCAGCTGTGACCAGAATGGGTG
  TTACTGGTGTGAAGAGGGCTTCTTTCTCTTAGGTGGCAGTTGTGTGAGGAAATGTGGTCCTGGATTCTAT
  GGTGACCAAGAAATGGGAGAATGTGAGTCCTGCCACCGAGCATGCGAAACCTGCACAGGCCCTGGTCATG
  ACGAGTGCAGCAGCTGCCAGGAAGGACTGCAGCTGCTGCGTGGGATGTGCGTGCATGCCACCAAGACCCA
  GGAGGAGGGCAAATTCTGGAATGATATTTTGAGAAAACTCCAGCCTTGTCATTCTTCTTGTAAAACCTGC
  AATGGATCTGCAACTCTGTGCACTTCATGTCCCAAAGGTGCATATCTTCTGGCTCAGGCCTGTGTTTCCT
  CCTGTCCCCAAGGCACATGGCCTTCCGTAAGGAGTGGGAGCTGCGAGAACTGTACGGAGGCCTGTGCCAT
  CTGCTCTGGAGCCGATCTTTGCAAAAAATGCCAGATGCAGCCGGGCCACCCTCTCTTCCTCCATGAAGGC
  AGGTGCTACTCCAAGTGCCCGGAGGGCTCTTATGCAGAAGACGGCATATGTGAACGCTGTAGCTCTCCTT
  GCAGAACATGTGAAGGAAACGCCACCAACTGCCATTCTTGTGAAGGAGGCCACGTCCTGCACCACGGAGT
  GTGCCAGGAAAACTGCCCCGAGAGGCACGTGGCTGTGAAGGGGGTATGCAAGCATTGCCCAGAGATGTGT
  CAGGACTGCATCCATGAGAAAACATGCAAAGAGTGCACGCCTGAGTTCTTCCTGCACGATGATATGTGCC
  ACCAGTCCTGTCCCCGTGGCTTCTATGCAGACTCGCGCCACTGTGTCCCCTGCCATAAAGACTGTCTGGA
  GTGCAGTGGCCCCAAAGCCGACGACTGCGAGCTCTGTCTTGAGAGTTCCTGGGTCCTCTATGATGGACTG
  TGCTTGGAGGAGTGTCCAGCAGGAACCTATTATGAAAAGGAGACTAAGGAGTGCAGAGATTGCCACAAGT
  CCTGCTTGACCTGCTCATCATCTGGGACCTGCACCACCTGTCAGAAAGGCCTGATCATGAACCCTCGTGG
  GAGCTGCATGGCCAACGAGAAGTGCTCACCCTCCGAGTACTGGGATGAGGATGCTCCCGGGTGCAAGCCC
  TGCCATGTTAAGTGCTTCCACTGCATGGGGCCGGCGGAGGACCAGTGTCAAACATGCCCCATGAACAGCC
  TTCTTCTCAACACAACCTGTGTGAAGGACTGCCCAGAGGGCTATTATGCCGATGAGGACAGCAACCGGTG
  TGCCCACTGCCACAGCTCTTGCAGGACATGTGAAGGGAGACACAGCAGGCAGTGCCACTCCTGCCGACCG
  GGCTGGTTCCAGCTAGGAAAAGAGTGCCTGCTCCAGTGCAGGGAAGGATATTACGCAGACAACTCCACTG
  GCCGGTGTGAGAGGTGCAACAGGAGCTGCAAGGGGTGCCAGGGCCCACGGCCCACAGACTGCCTGTCTTG
  CGATAGATTTTTCTTTCTGCTCCGCTCCAAAGGAGAGTGTCATCGCTCCTGCCCAGACCATTACTATGTA
  GAGCAAAGCACACAGACCTGTGAGAGATGCCATCCGACTTGTGATCAATGCAAAGGAAAAGGAGCGTTGA
  ATTGTTTATCCTGTGTGTGGAGTTACCACCTCATGGGAGGGATCTGCACCTCGGACTGTCTTGTGGGGGA
  ATACAGAGTGGGAGAGGGAGAGAAGTTTAACTGTGAAAAATGCCACGAGAGCTGCATGGAATGCAAGGGA
  CCAGGGGCCAAGAACTGCACCTTGTGCCCTGCCAACCTGGTGCTGCACATGGACGACAGCCACTGCCTCC
  ACTGCTGCAACACCTCTGATCCCCCCAGTGCCCAGGAGTGCTGTGACTGCCAGGACACCACGGACGAATG
  CATCCTTCGAACAAGCAAGGTTAGGCCTGCAACTGAGCATTTCAAGACAGCTCTGTTCATCACCTCCTCC
  ATGATGCTGGTGCTTCTGCTCGGGGCAGCTGTGGTAGTGTGGAAGAAATCTCGTGGCCGAGTCCAGCCAG
  CAGCAAAGGCCGGCTATGAAAAACTGGCCGACCCCAACAAGTCTTACTCCTCCTATAAGAGCAGCTATAG
  AGAGAGCACCAGCTTTGAAGAGGATCAGGTGATTGAGTACAGGGATCGGGACTATGATGAGGATGATGAT
  GATGACATCGTCTACATGGGCCAGGATGGCACAGTCTACCGGAAATTTAAATATGGGCTGCTGGATGACG
  ATGACATAGATGAGCTGGAATATGATGACGAGAGTTACTCCTACTACCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231494 representing NM_001190482
  Red=Cloning site Green=Tags(s)
  
  MGWGSRCCCPGRLDLLCVLALLGGCLLPVCRTRVYTNHWAVKIAGGFPEANRIASKYGFINIGQIGALKD
  YYHFYHSRTIKRSVISSRGTHSFISMEPKVEWIQQQVVKKRTKRDYDFSRAQSTYFNDPKWPSMWYMHCS
  DNTHPCQSDMNIEGAWKRGYTGKNIVVTILDDGIERTHPDLMQNYDALASCDVNGNDLDPMPRYDASNEN
  KHGTRCAGEVAAAANNSHCTVGIAFNAKIGGVRMLDGDVTDMVEAKSVSFNPQHVHIYSASWGPDDDGKT
  VDGPAPLTRQAFENGVRMGRRGLGSVFVWASGNGGRSKDHCSCDGYTNSIYTISISSTAESGKKPWYLEE
  CSSTLATTYSSGESYDKKIITTDLRQRCTDNHTGTSASAPMAAGIIALALEANPFLTWRDVQHVIVRTSR
  AGHLNANDWKTNAAGFKVSHLYGFGLMDAEAMVMEAEKWTTVPRQHVCVESTDRQIKTIRPNSAVRSIYK
  ASGCSDNPNRHVNYLEHVVVRITITHPRRGDLAIYLTSPSGTRSQLLANRLFDHSMEGFKNWEFMTIHCW
  GERAAGDWVLEVYDTPSQLRNFKTPGKLKEWSLVLYGTSVQPYSPTNEFPKVERFRYSRVEDPTDDYGTE
  DYAGPCDPECSEVGCDGPGPDHCNDCLHYYYKLKNNTRICVSSCPPGHYHADKKRCRKCAPNCESCFGSH
  GDQCMSCKYGYFLNEETNSCVTHCPDGSYQDTKKNLCRKCSENCKTCTEFHNCTECRDGLSLQGSRCSVS
  CEDGRYFNGQDCQPCHRFCATCAGAGADGCINCTEGYFMEDGRCVQSCSISYYFDHSSENGYKSCKKCDI
  SCLTCNGPGFKNCTSCPSGYLLDLGMCQMGAICKDGEYVDEHGHCQTCEASCAKCQGPTQEDCTTCPMTR
  IFDDGRCVSNCPSWKFEFENQCHPCHHTCQRCQGSGPTHCTSCGADNYGREHFLYQGECGDSCPEGHYAT
  EGNTCLPCPDNCELCHSVHVCTRCMKGYFIAPTNHTCQKLECGQGEVQDPDYEECVPCEEGCLGCSLDDP
  GTCTSCAMGYYRFDHHCYKTCPEKTYSEEVECKACDSNCGSCDQNGCYWCEEGFFLLGGSCVRKCGPGFY
  GDQEMGECESCHRACETCTGPGHDECSSCQEGLQLLRGMCVHATKTQEEGKFWNDILRKLQPCHSSCKTC
  NGSATLCTSCPKGAYLLAQACVSSCPQGTWPSVRSGSCENCTEACAICSGADLCKKCQMQPGHPLFLHEG
  RCYSKCPEGSYAEDGICERCSSPCRTCEGNATNCHSCEGGHVLHHGVCQENCPERHVAVKGVCKHCPEMC
  QDCIHEKTCKECTPEFFLHDDMCHQSCPRGFYADSRHCVPCHKDCLECSGPKADDCELCLESSWVLYDGL
  CLEECPAGTYYEKETKECRDCHKSCLTCSSSGTCTTCQKGLIMNPRGSCMANEKCSPSEYWDEDAPGCKP
  CHVKCFHCMGPAEDQCQTCPMNSLLLNTTCVKDCPEGYYADEDSNRCAHCHSSCRTCEGRHSRQCHSCRP
  GWFQLGKECLLQCREGYYADNSTGRCERCNRSCKGCQGPRPTDCLSCDRFFFLLRSKGECHRSCPDHYYV
  EQSTQTCERCHPTCDQCKGKGALNCLSCVWSYHLMGGICTSDCLVGEYRVGEGEKFNCEKCHESCMECKG
  PGAKNCTLCPANLVLHMDDSHCLHCCNTSDPPSAQECCDCQDTTDECILRTSKVRPATEHFKTALFITSS
  MMLVLLLGAAVVVWKKSRGRVQPAAKAGYEKLADPNKSYSSYKSSYRESTSFEEDQVIEYRDRDYDEDDD
  DDIVYMGQDGTVYRKFKYGLLDDDDIDELEYDDESYSYYQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190482
• ORF Size: 5580 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190482.1, NP_001177411.1
• RefSeq Size: 9538 bp
• RefSeq ORF: 5583 bp
• Locus ID: 5125
• Cytogenetics: 9q21.13
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: 'This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER. It then sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. This encoded protein is widely expressed and one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It mediates posttranslational endoproteolytic processing for several integrin alpha subunits and is thought to process prorenin, pro-membrane type-1 matrix metalloproteinase and HIV-1 glycoprotein gp160. Alternative splicing results in multiple transcript variants, some of which encode distinct isoforms, including a protease packaged into dense core granules (PC5A) and a type 1 membrane bound protease (PC5B). [provided by RefSeq, May 2014]'