EXTL2 (NM_001261440) Human Tagged ORF Clone

CAT#: RG232494

EXTL2 (GFP-tagged) - Homo sapiens exostosin-like glycosyltransferase 2 (EXTL2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EXTL2
• Synonyms: EXTR2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232494 representing NM_001261440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGTTGCCACATCTGCAAACTTCCTGGGAGAGTAATGGGGATTCGAGTGCTTCGATTATCTTTGG
  TGGTCATCCTCGTATTATTACTGGTAGCTGGTGCTTTGACTGCCTTACTTCCCAGTGTTAAAGAAGACAA
  GATGCTCATGTTGCGTAGGGAAATAAAATCCCAGGGCAAGTCCACCATGGACTCCTTTACTCTCATAATG
  CAGACGTACAACAGAACAGATCTCTTATTGAAACTTTTAAATCATTATCAGGCTGTACCAAATCTGCACA
  AAGTGATTGTGGTATGGAACAATATTGGAGAGAAGGCACCAGATGAATTATGGAATTCTCTAGGGCCCCA
  CCCTATCCCTGTGATCTTCAAACAACAGACAGCAAACAGGATGAGAAATCGACTCCAGGTCTTTCCTGAA
  CTGGAAACCAATGTGTTGATGGTAGATGATGACACACTCATCAGCACCCCAGACCTTGTTTTTGCTTTCT
  CAGTTTGGCAGCAATTTCCTGATCAAATTGTAGGATTTGTTCCTAGAAAGCACGTCTCTACTTCATCAGG
  TATCTACAGTTATGGAAGTTTTGAAATGCAAGCACCAGGGTCTGGAAATGGTGACCAGTACTCTATGGTG
  CTGATTGGAGCCTCATTCTTCAATAGCAAATATCTTGAATTATTTCAGAGGCAACCTGCAGCTGTCCATG
  CTTTGATAGATGATACTCAAAACTGTGATGATATTGCCATGAATTTTATCATTGCCAAGCATATTGGCAA
  GACTTCAGGGATATTTGTGAAGCCTGTAAACATGGACAATTTGGAAAAAGAAACCAACAGTGGCTATTCT
  GGAATGTGGCATCGAGCTGAGCACGCTCTGCAGAGGTCTTATTGTATAAATAAGCTTGTTAATATCTATG
  ATAGCATGCCCTTAAGATACTCCAACATTATGATTTCCCAGTTTGGTTTTCCATATGCCAACTACAAAAG
  AAAAATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232494 representing NM_001261440
  Red=Cloning site Green=Tags(s)
  
  MRCCHICKLPGRVMGIRVLRLSLVVILVLLLVAGALTALLPSVKEDKMLMLRREIKSQGKSTMDSFTLIM
  QTYNRTDLLLKLLNHYQAVPNLHKVIVVWNNIGEKAPDELWNSLGPHPIPVIFKQQTANRMRNRLQVFPE
  LETNVLMVDDDTLISTPDLVFAFSVWQQFPDQIVGFVPRKHVSTSSGIYSYGSFEMQAPGSGNGDQYSMV
  LIGASFFNSKYLELFQRQPAAVHALIDDTQNCDDIAMNFIIAKHIGKTSGIFVKPVNMDNLEKETNSGYS
  GMWHRAEHALQRSYCINKLVNIYDSMPLRYSNIMISQFGFPYANYKRKI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001261440
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001261440.1, NP_001248369.1
• RefSeq Size: 3178 bp
• RefSeq ORF: 990 bp
• Locus ID: 2135
• Cytogenetics: 1p21.2
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis
• Gene Summary: ''